Role of lipoxygenase and allene oxide synthase in wound-inducible defense response of pea

Yang, Hao-Ru; Liu, H. T.; Tang, Ke; Huang, Weidong

doi:10.1134/s1021443711020233

Cited by 7 publications

(8 citation statements)

References 26 publications

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“…; Yang et al . ), however, the involvement of PAL in lignin synthesis and lodging resistance has not so far been explored in any detail. Thus, it might not be accidental that PAL becomes a causal link between wounding and lignin synthesis.…”

Section: Discussionmentioning

confidence: 99%

Double benefits of mechanical wounding in enhancing chilling tolerance and lodging resistance in wheat plants

Wang

Huang

et al. 2019

Plant Biol J

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Chilling and lodging are major threats to wheat production. However, strategies that can be used to effectively mitigate the adverse effects of these threats are still far from clear. Mechanical wounding is a traditional agronomic measure, whereas information about the role it plays in wheat chilling and lodging is scant.• The aim of the present study was to investigate mechanisms underlying the protective roles of mechanical wounding in alleviating damage from chilling at jointing stage and enhancing lodging resistance after anthesis of winter wheat (Triticum aestivum L.).• Our data show that net photosynthesis rate, maximum photochemical efficiency of photosystem II, activity of the antioxidant enzymes and osmolytes were significantly increased in the latest fully expanded leaves of wounded plants under chilling. Wounding also reduced hydrogen peroxide accumulation, electrolyte leakage and water loss in wounded plants. Moreover, mechanical wounding significantly reduced the length but increased the diameter and wall thickness of the basal second internode of the main stem. Quantitative and histochemical analysis further indicated that wounding increased lignin accumulation and activity of enzymes involved in lignin synthesis, which resulted in increased mechanical strength and the lodging resistance index in the main stem.• We conclude from our data that mechanical wounding confers both cold tolerance by alleviating the damage caused by chilling at jointing stage and lodging resistance after anthesis of wheat plants.

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Section: Discussionmentioning

confidence: 99%

Double benefits of mechanical wounding in enhancing chilling tolerance and lodging resistance in wheat plants

Wang

Huang

et al. 2019

Plant Biol J

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“…Exogenous JA stimulated transcription of the AOS gene in rice during the development of resistance to Magnaporthe grisea (Mei et al, 2006). JA content in potato plants correlated with transcriptional activity of the AOS gene, and enzymatic activity of AOS (Yang et al, 2011). Potato plants have an OPR gene encoding the peroxisomal polypeptide 43.9 kDa and pI c. 7.72, which showed maximal levels at 2 h post-wounding (D ıaz et al, 2012), whereas the maximal transcript levels of the AOS gene was observed at 4 h hours post-wounding (Mei et al, 2006).…”

Section: Discussionmentioning

confidence: 93%

“…Exogenous JA stimulated transcription of the AOS gene in rice during the development of resistance to Magnaporthe grisea (Mei et al ., ). JA content in potato plants correlated with transcriptional activity of the AOS gene, and enzymatic activity of AOS (Yang et al ., ). Potato plants have an OPR gene encoding the peroxisomal polypeptide 43.9 kDa and pI c .…”

Section: Discussionmentioning

confidence: 97%

“…H 2 O 2 restored asAPO plant resistance to WT plant levels. This could be explained by the increased level of JA-dependent gene (AOS, OPR and PR-6) transcripts, which have been shown to be very important for plant resistance to a broad spectrum of pathogens (Yang et al, 2011;D ıaz et al, 2012). Exogenous JA stimulated transcription of the AOS gene in rice during the development of resistance to Magnaporthe grisea (Mei et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Anionic peroxidase‐mediated oxidative burst requirement for jasmonic acid‐dependentSolanum tuberosumdefence againstPhytophthora infestans

2017

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Plants are subject to pervasive attack by a diversity of pathogens and herbivores. It is considered that salicylic (SA) and jasmonic (JA) acids induce systemic resistance to these antagonists. Different lines of evidence indicate that the interplay of SA and JA with reactive oxygen species (ROS), generated by NADPH oxidases or apoplastic peroxidases, has an important role in the plant defence response. It was previously shown that diminished expression of the Solanum tuberosum anionic peroxidase gene M21334, as a consequence of antisense expression (asAPO plants), results in high susceptibility to Phytophthora infestans and deficient lignin deposition in cell walls. Further research reported in this paper showed that asAPO antisense plants have a low level of H2O2 and impaired JA‐associated responses, such as reduced transcription of JA‐responsive and JA‐biosynthesis genes. The asAPO plants did not exhibit any apparent defect in their susceptibility to SA. Exogenous H2O2 partially restored JA‐dependent reactions in asAPO plants, together with an increase in lignin deposition around pathogen‐penetrated sites, resulting in increased resistance of asAPO to P. infestans. These data demonstrate that the anionic peroxidase‐dependent oxidative burst plays an important role in the elicitation of JA‐associated immunity.

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“…This signaling molecule led to an increase in LOX activity as well as endogenous JA content (Figure 1). Time course experiments (Figure 2) showed that the LOX activity was clearly detectable in the wheat callus after 6 h exposed to MeJA and increased continuously for the next 18 h. Furthermore, SHAM, which is an efficient inhibitor of JA biosynthesis, could suppress LOX activity and gene expression (Kn fel et al, ӧ 1984;Gao et al, 2003;Yang et al, 2011). It was reported that SHAM inhibit LOX activity by 90% and prevent the synthesis of the vegetative storage protein mRNA (Macrì et al, 1994;Staswick et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

Ma¹,

Wang²,

Sun³

et al. 2015

JAS

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Drought stress is a major challenge in agriculture, causing severe loss of crops throughout the world. Methyl jasmonate (MeJA) regulates a variety of plant developmental process and responds to many biotic and abiotic stresses. To explore the physiological mechanisms of drought stress mitigated by exogenous MeJA, we subjected callus of wheat (Triticum aestivum L.) to drought induced medium after pretreatment with 10 ml sterile solution that contained the following singly or in combinations: MeJA (0, 0.25, and 2.5µM) and the lipoxygenase (LOX) inhibitor salicylhydroxamic acid (SHAM; 0 and 0.5 mM) for 24 hours. The endogenous jasmonic acid (JA) content, activity of LOX, hydrogen peroxide (H 2 O 2 ) content, antioxidant enzymes activities, levels of reactive oxygen species (ROS), content of malondialdehyde (MDA), and cell viability were measured. The results showed that exogenous MeJA induced the activity of LOX and resulted in rapid increase in endogenous JA levels in a concentration-dependent manner; JA declined gradually after reaching its maximum. The level of H 2 O 2 increased with increases in LOX activity and endogenous JA level, which are involved in the octadecanoid signaling pathway. However, SHAM inhibited the LOX activity, the endogenous JA level, and the accumulation of H 2 O 2 in wheat callus. In addition, the activities of several antioxidant enzymes increased after MeJA-pretreatment, but this effect was also inhibited by SHAM. The cumulative results suggest that H 2 O 2 was generated through the octadecanoid signaling pathway, and some antioxidant defense genes were provoked by the accumulation of H 2 O 2 . Therefore, we inferred that H 2 O 2 might act as a signaling molecule for the MeJA-induced antioxidant defense. Drought stress can be improved by MeJA-pretreatment, leading to decrease in endogenous MDA and ROS contents, which are normally induced by drought conditions. Both drought stress and exogenous MeJA-pretreatment increased in LOX activity, endogenous JA level, and antioxidant enzymes. Combinatorial treatments showed an apparent synergistic effect on the activities of these antioxidant enzymes and resulted in improved cell viability. These results confirmed the hypothesis that H 2 O 2 acts as a signaling molecule for the MeJA-induced antioxidant defense and could alleviate the negative effects of drought stress on wheat callus.

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Role of lipoxygenase and allene oxide synthase in wound-inducible defense response of pea

Cited by 7 publications

References 26 publications

Double benefits of mechanical wounding in enhancing chilling tolerance and lodging resistance in wheat plants

Double benefits of mechanical wounding in enhancing chilling tolerance and lodging resistance in wheat plants

Anionic peroxidase‐mediated oxidative burst requirement for jasmonic acid‐dependentSolanum tuberosumdefence againstPhytophthora infestans

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

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