Role of lncRNA‑ATB in ovarian cancer and its mechanisms of action

Yuan, Donglan; Zhang, Xiaofang; Zhao, Yongmei; Hua, Qian; Wang, Hezhu; He, Cuiqin; Liu, Xia; Guo, Ting; Lin, Mei; Yu, Hong; Ye, Jun

doi:10.3892/etm.2019.8282

Cited by 11 publications

(7 citation statements)

References 43 publications

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“…Long non-coding RNAs (lncRNAs) are endogenous and long non-coding RNA molecules (~200nt) that regulate gene expression by targeting miRNA or mRNA posttranscriptionally. [4][5][6][7] Increasing studies have revealed lncRNAs played crucial roles in tumorigenesis, metastasis and MDR. For example, lncRNA DANCR promotes tumor progression and cancer stemness features in osteosarcoma by upregulating AXL via miR-33a-5p inhibition.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA SLC25A21-AS1 Promotes Multidrug Resistance in Nasopharyngeal Carcinoma by Regulating miR-324-3p/IL-6 Axis</p>

Wang¹,

Wang²,

Xu³

et al. 2020

CMAR

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Background: Nasopharyngeal carcinoma (NPC), one of the most common types of head and neck tumor, occurred in the epithelial lining of the nasopharynx and is mainly prevalent in Southeast Asia and Southern China. However, the molecular mechanisms of NPC multidrug resistance still remained largely unclear. Methods: The qRT-PCR assay was performed to examine SLC25A21-AS1, miR-324-3p and IL-6 expression in NPC tissues and cell. The CCK8 assay and colony formation assay were used to detect cell growth. In addition, CCK8 assay was performed to detect IC 50 values of different drugs in NPC cell. Results: In this study, we found that SLC25A21-AS1 expression was increased in NPC tissues and cell line, and knockdown of SLC25A21-AS1 inhibited cell growth and MDR in NPC cell. Moreover, SLC25A21-AS1 acted as a ceRNA for miR-324-3p and facilitates NPC cell growth and MDR by regulating the miR-324-3p/IL-6 axis. Conclusion: Our findings demonstrated the role of SLC25A21-AS1/miR-324-3p/IL-6 axis in cell growth and MDR in NPC, which might be a potential prognostic and diagnostic marker in NPC patients and provide new insight into the molecular mechanism of MDR in NPC chemotherapy.

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Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA SLC25A21-AS1 Promotes Multidrug Resistance in Nasopharyngeal Carcinoma by Regulating miR-324-3p/IL-6 Axis</p>

Wang¹,

Wang²,

Xu³

et al. 2020

CMAR

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“…Long non‐coding RNAs (lncRNAs) could transcriptionally or post‐transcriptionally regulate expression and epigenetic levels of target genes, whose dysregulation are widely known to be involved in the development and progression of many cancers 9 . Large numbers of lncRNAs regulate tumor progression in ovarian cancer 10 . For example, lncRNA SNHG20 promotes tumor progression, 11 while AOC4P suppresses metastasis of ovarian cancer 12 .…”

Section: Introductionmentioning

confidence: 99%

SP1‐induced lncRNA DANCR contributes to proliferation and invasion of ovarian cancer

Cui

et al. 2020

The Kaohsiung J of Med Scie

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Transcription factor SP1 could manipulate pathways involved in ovarian cancer progression. LncRNAs are involved in SP1‐mediated tumorigenesis. LncRNA DANCR could promote metastasis of ovarian cancer. However, the regulatory function and involvement of SP1‐induced lncRNA DANCR in ovarian cancer remain elusive. Data from this study showed that SP1 was up‐regulated in ovarian cancer tissues and cells (CAOV3, SKOV3, A2780), and SP1 could bind to the promoter region of DANCR through chromatin immunoprecipitation and leuciferase activity assays. Therefore, DANCR was transcriptionally induced by SP1 in ovarian cancer tissues and cells (CAOV3, SKOV3, A2780). Functionally, reduced expression of DANCR suppressed cell viability, migration and invasion of CAOV3, while enhanced DANCR expression contributed to SKOV3 growth. Over‐expression of SP1 reversed the suppressive effects of DANCR interference on ovarian cancer progression. In conclusion, SP1‐induced DANCR contributed to oncogenic potential of ovarian cancer, suggesting a promising therapeutic target for ovarian cancer.

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“…Increasing evidence indicates that early detection and prevention of ovarian cancer is an investigation priority as diagnosis at a local stage is accompanied with a 5-year relative survival rate of upper than 90% (Torre et al, 2018). There is considerable evidence showing that lncRNAs play critical roles in tumorigenesis process, invasion, and metastasis of ovarian cancer (Liu et al, 2020;Pa et al, 2020;Yuan et al, 2020). HOTAIRM1 has been proved to be aberrantly up/downregulated in some solid tumors (Zhang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Investigating function of long noncoding RNA of HOTAIRM1 in progression of SKOV3 ovarian cancer cells

Xia

Xiang

et al. 2021

Drug Development Research

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Ovarian cancer is one of the most heterogeneous malignancies in the field of gynecologic oncology. Deregulation of long noncoding RNAs (lncRNAs) is implicated in carcinogenesis. Therefore, the present study was conducted to investigate the possible role of lncRNA of HOXA transcript antisense intergenic RNA myeloid-specific 1 (HOTAIRM1) in progression of SKOV3 cells in ovarian cancer and also its underlying molecular mechanisms. HOTAIRM1 expression level will be measured by real-time polymerase chain reaction (PCR) in SKOV3 cells. For determining the effect of HOTAIRM1 silencing on progression of SKOV3 cells, siHOTAIRM1 will be designed and transfected into cells using a liposomal approach. MTT and trypan blue assays will be used to determine the effect of HOTAIRM1 silencing on cell proliferation.Apoptosis of the cells will be detected by flow cytometry. Furthermore, expressions of apoptosis-related genes and Wnt pathway-related proteins and genes will be analyzed by Western blot and real-time PCR. HOTAIRM1 was overexpressed in SKOV3 cells. Silencing of HOTAIRM1 alleviated cell proliferation, and increased cell apoptosis of SKOV3 cells. Moreover, siHOTAIRM1 significantly increased expression of pro-apoptotic agents, such as Bad and Bax, while it decreased expressions of Bid and Bcl-2 (anti-apoptotic agents). Also, silencing of HOTAIRM1 resulted in a suppressed expression of Wnt pathway-related proteins and also expression of its downstream target gene, matrix metalloproteinase 9(MMP9). Our findings provided new insights into function of lncRNA of HOTAIRM1 in progression of ovarian cancer by modulating Wnt pathway and its downstream target gene, MMP9.

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Role of lncRNA‑ATB in ovarian cancer and its mechanisms of action

Cited by 11 publications

References 43 publications

<p>Long Non-Coding RNA SLC25A21-AS1 Promotes Multidrug Resistance in Nasopharyngeal Carcinoma by Regulating miR-324-3p/IL-6 Axis</p>

<p>Long Non-Coding RNA SLC25A21-AS1 Promotes Multidrug Resistance in Nasopharyngeal Carcinoma by Regulating miR-324-3p/IL-6 Axis</p>

SP1‐induced lncRNA DANCR contributes to proliferation and invasion of ovarian cancer

Investigating function of long noncoding RNA of HOTAIRM1 in progression of SKOV3 ovarian cancer cells

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