Role of lncRNA NR2F1-AS1 and lncRNA H19 Genes in Hepatocellular Carcinoma and Their Effects on Biological Function of Huh-7

Abstract

Objective This research was designed to probe into the expression and related mechanism of lncRNA NR2F1-AS1 and H19 in hepatocellular carcinoma (HCC). Methods Forty-two HCC patients who came to our hospital from February 2018 to August 2019 were included into a research group (RG). Meanwhile, 46 healthy controls were regarded as a control group (CG). BEL-7402, Huh-7 human hepatoma cells and HL-7702 human normal liver cells were purchased, and the NR2F1-AS1 and H19 levels in serum and tissues of HCC patients were detected. PcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19 and si-H19 were transfected into BEL-7402 and Huh-7 cells. The NR2F1-AS1 and H19 levels in samples were detected via qRT-PCR, and the expression of apoptosis-related proteins in cells was tested through WB. Cell proliferation, invasion, or apoptosis was detected by CCK8, Transwell or flow cytometry, respectively. Results The NR2F1-AS1 and H19 levels were high in human hepatoma cells, and AUCs of lncRNA NR2F1-AS1 and lncRNA H19 were both >0.8. The lncRNA NR2F1-AS1 and lncRNA H19 were associated with HCC staging. After transfection of pcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19, si-H19 BEL-7402 and Huh-7 cells, silencing NR2F1-AS1 and H19 expression can promote apoptosis and inhibit cell growth, while silencing their over-expression can inhibit the EMT process of Huh-7 cells. Conclusion lncRNA NR2F1-AS1 and lncRNA H19 genes are abnormally expressed in HCC. Furthermore, the two can suppress the EMT process of Huh-7 cells and promote apoptosis effectively.

RETRACTED: NR2F1-AS1/miR-190a/PHLDB2 Induces the Epithelial–Mesenchymal Transformation Process in Gastric Cancer by Promoting Phosphorylation of AKT3

“…NR2F1-AS1 is a type of lncRNA. NR2F1-AS1 has been shown to facilitate malignant tumor development in non-small cell lung cancer (Zhang C. et al, 2020), breast cancer (Zhang Q. et al, 2020), bone cancer (Li et al, 2019), thyroid cancer (Guo et al, 2019), and endometrial cancer (Wang et al, 2019), as well as tumor cell EMT in liver cancer (Ji et al, 2021) and esophageal squamous cell cancer (Wang et al, 2019). However, the function and mechanism of NR2F1-AS1 in GC are unclear.…”

Long noncoding RNA NR2F1-AS1 plays a carcinogenic role in gastric cancer by recruiting transcriptional factor SPI1 to upregulate ST8SIA1 expression

“…A large number of studies have shown that lncRNAs are extensively involved in the biological processes of cancer cells and other cells [ 16 , 20 , 21 ]. A previous study found that NR2F1-AS1 expression was significantly upregulated in oxaliplatin-resistant hepatocellular carcinoma tissues [ 22 ]. Another study has shown that NR2F1-AS1 was highly expressed in hepatocellular carcinoma and its expression was found highly correlated with staging [ 22 ].…”

“…A previous study found that NR2F1-AS1 expression was significantly upregulated in oxaliplatin-resistant hepatocellular carcinoma tissues [ 22 ]. Another study has shown that NR2F1-AS1 was highly expressed in hepatocellular carcinoma and its expression was found highly correlated with staging [ 22 ]. Moreover, Li et al found that the NR2F1-AS1/miR-140/ hexokinase 2 axis regulated hypoxia-induced glycolysis and migration in hepatocellular carcinoma [ 23 ].…”

The Effect and Mechanism of lncRNA NR2F1-As1/miR-493-5p/MAP3K2 Axis in the Progression of Gastric Cancer

“…NR2F1-AS1 is verified to be aberrantly expressed in several human tumors and plays an important role in the development of tumors. NR2F1-AS1 was upregulated in hepatocellular carcinoma (HCC), and downregulated NR2F1-AS1 was found to suppress cell growth and EMT progression and promote cell apoptosis of HCC cells [23]. Moreover, high NR2F1-AS1 expression was associated with poor OS, and silencing of NR2F1-AS1 blocked HCC cell hypoxiainduced glycolysis [24].…”