Role of long non-coding RNA NEAT1 in the prognosis of prostate cancer patients

Abstract

Prostate cancer is the second leading cause of cancer-related deaths among male population worldwide, its incidence and lethality steadily increase. Nuclear enriched abundant transcript 1 (NEAT1) is a long non-coding RNA (ncRNA), located on chromatin 11. It has been found to function as an oncogene in different kinds of cancer. However, until now, the clinical significance of NEAT1 has not been investigated in prostate cancer. Paired tissue specimens of prostate cancer and matched normal prostate tissues were obtained from 130 patients with prostate cancer between 2014 and 2019 at The Fourth Affiliated Hospital Zhejiang University, School of Medicine. Group means were compared using the Student t test. Chi-Squared test was used for analyzing the correlation of the expression of NEAT1 with clinicopathologic features of prostate cancer patients. Survival data was analyzed using the Kaplan–Meier estimate and log-rank P was calculated. Cox regression model was used for univariate and multivariate analysis for factors related to overall survival. The expression of NEAT1 was increased significantly in prostate cancer tissues, compared with adjacent normal prostate tissues (P < .001). NEAT1 expression was significantly associated with TNM stage (P = .005), lymph nodes metastasis (P = .005), distant metastasis(P = .003), and Gleason score (P = .001). Overall survival rate was significantly lower for prostate cancer patients with a high expression level of NEAT1 than those with a low NEAT1a expression level (P = .048). In multivariate analysis, the results showed that the expression of NEAT1 was an independent prognostic factor for overall patient survival (HR: 2.111, CI: 1.735–10.295, P = .039). In the present study, NEAT1 is identified as an important lncRNA that may predict the prognosis of patients with prostate cancer.

Circular RNAs and Their Linear Transcripts as Diagnostic and Prognostic Tissue Biomarkers in Prostate Cancer after Prostatectomy in Combination with Clinicopathological Factors

“…Furthermore, NEAT1 promotes the proliferation of PCa cells in connection with the steroid receptor co-activator (SCRC3) through the insulin-like growth factor 1 receptor/AKT serine/threonine kinase 1 (IGF1R/AKT) signaling pathway [105]. As shown in the present study using BCR as the clinical endpoint, Bai et al [95] reported increased expression of NEAT1 mRNA as being an independent prognostic factor for overall patient survival.…”

“…Moreover, the normalized expression of linNEAT1 was nearly 17,000-fold higher than the expression level of circNEAT1 (Figure 4 and Table S10), while the ratio between tumor and normal tissue was equal for both RNAs (circNEAT1: +1.70 vs. linNEAT1: +1.75). A possible explanation for the high linRNA expression could be the similarly abundant expression of the lncRNA NEAT1 (lncNEAT1) in PCa, which may be an indicator of the independent expression of circRNAs and linRNAs/lncRNAs [94,95]. However, because of the limited performance data from the circNEAT1 RT-qPCR analyses and the incomplete detection of this analyte in all samples, we did not include this circRNAs in the multivariable analyses.…”

“…Expression data that could be used for the differentiation of tissue samples as well as BCR markers are missing. Reference to possible biological functions has only been made in few cases, and the validity as a biomarker has only been considered for linNEAT1 [95]. Since our intention in this study was primarily to investigate the clinical validity of the selected RNAs as possible biomarkers, we deliberately refrained from undertaking functional investigations.…”

Roles of Noncoding RNAs in the Initiation and Progression of Myocardial Ischemia-Reperfusion Injury

“…The lncRNA NEAT1, retained in the nucleus, is transcribed from the MEN locus [102]. Over the past decades, research on lncRNA NEAT1 has mainly focused on cancers, such as colorectal cancer [103], prostate cancer [104], lung cancer [105] and gastric cancer [106]. Recently, Ma et al revealed that NEAT1 was expressed at high levels in the myocardial tissues of I/R-treated diabetic rats, and that upregulated NEAT1 expression was associated with deterioration of MIRI via the activation of apoptosis and autophagy, while the downregulation of NEAT1 alleviated MIRI by depressing autophagic flux and apoptosis [54].…”

Long noncoding RNA NEAT1 promotes tumorigenesis in H. pylori gastric cancer by sponging miR‐30a to regulate COX‐2/BCL9 pathway

“…15 Additionally, NEAT1 is aberrantly expressed in different types of cancer. High levels of NEAT1 may act as a biomarker of prostate cancer patients, 16 and NEAT1 promotes cervical cancer cell invasion and the progression of sarcoma metastasis. 17 NEAT1 is downregulated in acute promyelocytic leukemia.…”