Role of luminal lecithin in intestinal fat absorption

O’Doherty, P. J. A.; Kakis, G.; Kuksis, A.

doi:10.1007/bf02531899

Cited by 112 publications

(65 citation statements)

References 25 publications

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“…The unsuitability can be attributed to excessive fatty acids in the artificial feed, to excessive feeding facilitated by the consistency of the feed, which is abundantly provided, or to a deficiency in certain elements that are indispensable for the proper assimilation of lipids. For instance, it is well known that lecithins are important in the solubilization, absorption, and transport of fats in the mammalian intestine (O'Doherty et al 1973, Rodgers 1975, Tso et al 1977. One of lecithin components, choline, plays an essential role in lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Intestinal steatosis in the farm-reared sea bass Dicentrarchus labrax

Deplano¹,

Connes²,

Diaz³

et al. 1989

Dis. Aquat. Org.

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Light microscopy, scanning and transmission electron microscopy were used to study the anterior intestine of the sea bass Dicentrarchus labrax L., involved in lipid absorption. In the first part of the study, intestinal morphology of control feral fishes was described and lipid absorption through the intestinal mucosa was defined by comparing starved and fed individuals. In the second part, similar investigations were made on specimens reared on artificial food. These show injuries of the mucosa, ranging from simple impairment of enterocytic striated border to cellular necrosis and abrasion. The severity of injuries was related to the extent of lipid overloading within the mucosa. This overloadng was mainly represented by abundant lipid droplets in epithelial cells, and by the accumulation of numerous and large lipid granules in interenterocytic spaces and in the lamina propria. Possible influences of artificial feed on this digestive pathology are discussed from quantitative and qualitative points of view.

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Section: Discussionmentioning

confidence: 99%

Intestinal steatosis in the farm-reared sea bass Dicentrarchus labrax

Deplano¹,

Connes²,

Diaz³

et al. 1989

Dis. Aquat. Org.

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“…It is difficult to see how this was possible with tolerable volume of test meal (not described in their paper) or without an alkaline pH (Hofmann, 1968), well buffered against gastric acidity. However, the output of absorbed lipid as chylomicrons in lymph was not measured in the present study nor in that of O'Doherty et al (1973). Experiments are underway using a steady state lymphatic absorption method (Shepherd and Simmonds, 1959) and a more stable test meal.…”

Section: Mucosal Recoveriesmentioning

confidence: 71%

“…Recently, O'Doherty, Kakis and Kuksis (1973) suggested that dietary or biliary lecithin play an important role in the normal transport of absorbed fat out of the intestinal mucosa. They found that mucosal content of absorbed radioactive fatty acid was decreased, whereas that of liver increased when egg lecithin was added to a gastric test meal of lipid and bile salts fed to bile fistula rats.…”

Section: Introductionmentioning

confidence: 99%

Importance of Luminal Lecithin in Intestinal Absorption and Transport of Lipid in the Rat

Tso¹,

Simmonds²

1977

Aust J Exp Biol Med

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“…The major product of PC digestion, lysoPC, is water soluble and readily absorbed by enterocytes. It is a major source of enterocyte phospholipids for the generation of lipoproteins, and chylomicron formation as shown in the multidrug resistant Pglycoprotein knockout mice (23), for transport of triglyceride from the cell to lymph and for replenishment of membrane phospholipids used in exocytosis (12,13,24,25). Lack of cosecretion of PLA 2 could substantially impair these processes.…”

Section: Discussionmentioning

confidence: 99%

Parallel Secretion of Pancreatic Phospholipase A2, Phospholipase A1, Lipase, and Colipase in Children with Exocrine Pancreatic Dysfunction

Nouri-Sorkhabi¹,

Chapman

Kuchel

et al. 2000

Pediatr Res

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The cosecretion of pancreatic lipase and colipase are important in normal fat digestion. As adsorption of phosphatidylcholine to the lipid substrate interferes with lipase activity, hydrolysis to lysophosphatidylcholine with subsequent desorption is also essential for fat digestion. There are some data regarding the secretion of pancreatic phospholipases in normal adults but none in children or patients with pancreatic disease. In the present study, we aimed a) to develop an accurate fast assay method to measure phospholipase A 2 and b) to determine the secretion rate of pancreatic phospholipase A 2 and whether it is cosecreted with lipase and colipase in children with exocrine pancreatic dysfunction. Nine male patients aged 0.5 to 16 y (seven with cystic fibrosis, two with malabsorption) underwent pancreatic stimulation tests. Their colipase and lipase secretion rates were measured by titrimetric methods and phospholipase A 2 and A 1 by phosphorus magnetic resonance spectroscopy ( 31 P NMR). It was found that the phospholipases, colipase, and lipase were absent in the two patients with pancreatic insufficiency. In patients with normal absorption, there were marked inter-and intrasubject variations of lipase, colipase, and phospholipase secretion rates that were consistent with the degree of exocrine pancreatic dysfunction. However, in the three 20-min stimulation periods of the pancreatic function test, pancreatic phospholipase is cosecreted with lipase and colipase, and average colipase and phospholipase A 2 secretion rates follow a similar or parallel pattern. These findings are consistent with the important role of pancreatic phospholipases in intestinal phospholipid hydrolysis leading to the desorption of phospholipids from the lipid substrate and enhancing lipid hydrolysis and phospholipid absorption. Abbreviations CEH, carboxyl ester hydrolase CF, cystic fibrosis GPC, glycerophosphocholine lysoPC, lysophosphatidylcholine MCC, mucocutaneous candidiasis and polyendocrinopathy NMR, nuclear magnetic resonance PC, phosphatidylcholine PLA 1 , phospholipase A 1 PLA 2 , phospholipase A 2 PI, pancreatic insufficient PS, pancreatic sufficient Exocrine pancreatic function has been well characterized in children with pancreatic disease with the use of quantitative pancreatic function tests (1-3). To determine the relationship between residual pancreatic function and fat absorption, sensitive titrimetric assays were developed for lipase and the lipase cofactor colipase (4) and their secretion rates compared with fecal fat excretions in children with CF, Shwachman syndrome, or controls (3,5). These studies demonstrated that patients with normal fat absorption had a wide range of enzyme/coenzyme secretion from less than 5% up to within the normal control range; and the threshold for lipolytic failure was near 1% of average control colipase values, i.e. patients with values below this level had fat malabsorption and above this level normal absorption. These observations led to the currently accepted definition that patie...

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Role of luminal lecithin in intestinal fat absorption

Cited by 112 publications

References 25 publications

Intestinal steatosis in the farm-reared sea bass Dicentrarchus labrax

Intestinal steatosis in the farm-reared sea bass Dicentrarchus labrax

Importance of Luminal Lecithin in Intestinal Absorption and Transport of Lipid in the Rat

Parallel Secretion of Pancreatic Phospholipase A2, Phospholipase A1, Lipase, and Colipase in Children with Exocrine Pancreatic Dysfunction

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