1981
DOI: 10.1104/pp.67.5.875
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Role of Malate Synthase in Citric Acid Synthesis by Maturing Cotton Embryos: A Proposal

Abstract: Cotton embryos from 34 to 54 days after anthesis were analyzed for organic acids, and enzymes associated with organic acid metabolism. During this developmental period, embryos accumulated citrate. Malate synthase activity appeared at 46 days after anthesis and increased rapidly to 54 days. Of other enzymes examined, only citrate synthase activity increased during this period. As isocitrate lyase activity was absent from cotton embryos during maturation, an 3 To whom reprint requests should be addressed.vers… Show more

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Cited by 32 publications
(16 citation statements)
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“…Maximum velocities decreased nearly 1 OO-fold as acyl-chain length was increased from C4-C16. With any of the substrates examined, in vitro activity of enoyl hydratase was in excess of activity required for storage lipid mobilization [2], suggesting that the enoyl hydratase hydration is not the limiting step in fatty acid P-oxidation. …”
Section: Methodsmentioning
confidence: 99%
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“…Maximum velocities decreased nearly 1 OO-fold as acyl-chain length was increased from C4-C16. With any of the substrates examined, in vitro activity of enoyl hydratase was in excess of activity required for storage lipid mobilization [2], suggesting that the enoyl hydratase hydration is not the limiting step in fatty acid P-oxidation. …”
Section: Methodsmentioning
confidence: 99%
“…Bacteria also have long-and short-chain enoyl-CoA hydratases [ 15 ,I 61 which apparently operate separately in either the cytosol or plasma membrane [15]. In contrast, P-oxidation in cotton cotyledons is located exclusively in peroxisomes (glyoxysomes) [2], possibly reflecting the ability of cotton peroxisomal enzyme(s) to utilize a wide range of acyl chain lengths. The results in table 1 clearly illustrate the need to measure P-oxidation enzyme activity with variable chain length substrates (rather than with C4 only as is often done) before comparing enzyme activities among species, or relating in vitro activities to in vivo rates of fatty acid catabolism.…”
Section: Discussionmentioning
confidence: 99%
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