Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review

Pabbathi, Ninian Prem Prashanth; Velidandi, Aditya; Tavarna, Tanvi; Gupta, Shreyash; Raj, Ram Sarvesh; Gandam, Pradeep Kumar; Baadhe, Rama Raju

doi:10.1007/s13399-020-01186-y

Cited by 27 publications

(11 citation statements)

References 209 publications

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“…One approach that has been widely used is to look for specific genomic content in environmental samples through metagenomics [ 378 , 379 ]. To isolate new genes and pathways encoding enzymes or biosynthesis of biomolecules, functional metagenomics has been widely successful in isolating and identifying new families of proteins, especially lignocellulolytic enzymes [ 380 ]. Using metagenomics, therefore, allows the prospection of potential lignocellulolytic microorganisms very quickly, in addition to allowing the identification of both cultured and non-cultured microorganisms [ 381 ].…”

Section: Recent Advancesmentioning

confidence: 99%

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Benatti

Polizeli

2023

Microorganisms

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Human population growth, industrialization, and globalization have caused several pressures on the planet’s natural resources, culminating in the severe climate and environmental crisis which we are facing. Aiming to remedy and mitigate the impact of human activities on the environment, the use of lignocellulolytic enzymes for biofuel production, food, bioremediation, and other various industries, is presented as a more sustainable alternative. These enzymes are characterized as a group of enzymes capable of breaking down lignocellulosic biomass into its different monomer units, making it accessible for bioconversion into various products and applications in the most diverse industries. Among all the organisms that produce lignocellulolytic enzymes, microorganisms are seen as the primary sources for obtaining them. Therefore, this review proposes to discuss the fundamental aspects of the enzymes forming lignocellulolytic systems and the main microorganisms used to obtain them. In addition, different possible industrial applications for these enzymes will be discussed, as well as information about their production modes and considerations about recent advances and future perspectives in research in pursuit of expanding lignocellulolytic enzyme uses at an industrial scale.

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Section: Recent Advancesmentioning

confidence: 99%

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Benatti

Polizeli

2023

Microorganisms

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“…Some examples include turbomycin A and B, chitinase with chitobiosidase activity, lactonases, LuxR/LuxI genes, bacterial NAHLase, carboxylesterase, kanamycin, and ceftazidime resistance genes [ 53 , 64 ]. Metagenomics have also been used to discover novel endoglucanases for production of second-generation biofuel [ 65 ].…”

Section: Global Trends Of Metagenome Studiesmentioning

confidence: 99%

Microbiome Studies from Saudi Arabia over the Last 10 Years: Achievements, Gaps, and Future Directions

Alzahrani

2021

Microorganisms

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In the past ten years, microbiome studies have shown tremendous potentiality for implementation of understanding microbiome structures and functions of various biomes and application of this knowledge for human betterment. Saudi Arabia is full of geographical, ecological, ethnical, and industrial diversities and scientific capacities. Therefore, there is a great potential in Saudi Arabia to conduct and implement microbiome-based research and applications. However, there is no review available on where Saudi Arabia stands with respect to global microbiome research trends. This review highlights the metagenome-assisted microbiome research from Saudi Arabia compared to the global focuses on microbiome research. Further, it also highlights the gaps and areas that should be focused on by Saudi microbiome researchers and the possible initiatives to be taken by Saudi government and universities. This literature review shows that the global trends of microbiome research cover a broad spectrum of human and animal health conditions and diseases, environmental and antimicrobial resistance surveillance, surveillance of food and food processing, production of novel industrial enzymes and bioactive pharmaceutical products, and space applications. However, Saudi microbiome studies are mostly confined to very few aspects of health (human and animal) and environment/ecology in last ten years, without much application. Therefore, Saudi Arabia should focus more on applied microbiome research through government, academic, and industry initiatives and global cooperation to match the global trends.

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“…Although most of the known industrial enzymes are obtained from microorganisms through pure culture isolation and screening ( Martin and Vandenbol, 2016 ), recent estimates show that more than 99% of microorganisms in the environment are uncultivable through conventional microbiological methods ( Berini et al, 2017a ). Thus, in recent years the advent of metagenomics has provided a powerful tool to access the genetic and metabolic diversity of microorganisms in any environment, bypassing the limitations of the current culture-dependent approaches ( Berini et al, 2017a ; Ngara and Zhang, 2018 ; Pabbathi et al, 2021 ). Functional metagenomics is a method that clones the environmental DNA into a suitable vector to create a library and which is then transformed into a host, such as E. coli .…”

Section: Introductionmentioning

confidence: 99%

Discovery of novel carbohydrate degrading enzymes from soda lakes through functional metagenomics

et al. 2022

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Extremophiles provide a one-of-a-kind source of enzymes with properties that allow them to endure the rigorous industrial conversion of lignocellulose biomass into fermentable sugars. However, the fact that most of these organisms fail to grow under typical culture conditions limits the accessibility to these enzymes. In this study, we employed a functional metagenomics approach to identify carbohydrate-degrading enzymes from Ethiopian soda lakes, which are extreme environments harboring a high microbial diversity. Out of 21,000 clones screened for the five carbohydrate hydrolyzing enzymes, 408 clones were found positive. Cellulase and amylase, gave high hit ratio of 1:75 and 1:280, respectively. A total of 378 genes involved in the degradation of complex carbohydrates were identified by combining high-throughput sequencing of 22 selected clones and bioinformatics analysis using a customized workflow. Around 41% of the annotated genes belonged to the Glycoside Hydrolases (GH). Multiple GHs were identified, indicating the potential to discover novel CAZymes useful for the enzymatic degradation of lignocellulose biomass from the Ethiopian soda Lakes. More than 73% of the annotated GH genes were linked to bacterial origins, with Halomonas as the most likely source. Biochemical characterization of the three enzymes from the selected clones (amylase, cellulase, and pectinase) showed that they are active in elevated temperatures, high pH, and high salt concentrations. These properties strongly indicate that the evaluated enzymes have the potential to be used for applications in various industrial processes, particularly in biorefinery for lignocellulose biomass conversion.

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Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review

Cited by 27 publications

References 209 publications

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Microbiome Studies from Saudi Arabia over the Last 10 Years: Achievements, Gaps, and Future Directions

Discovery of novel carbohydrate degrading enzymes from soda lakes through functional metagenomics

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