ROLE OF METALLOPROTEINASE-12-82A>G GENE SNP IN THE PATHOGENESIS OF COMMUNITY-ACQUIRED PNEUMONIA IN CONTRACTION-AGE PATIENTS

Abstract

The purpose of the study is to establish the significance of the MMP12-82A>G gene SNP in the pathogenesis of community-acquired pneumonia in patients of military age.Materials and methods. A survey of 160 unrelated patients of military age (18-20 years) living in the TransBaikal Territory was carried out. The first group (n=80) - patients with COVID-19 infection complicated by mild pneumonia (n=40) and severe pneumonia (n=40). Group 2 – clinical comparison (n=80) – patients with acute respiratory infection (ARI) of non-influenza etiology, complicated by mild pneumonia (n=40) and severe pneumonia (n=40). The control group consisted of 86 practically healthy men of the same age. Exclusion criteria: presence of family ties; patients with acute and/or chronic concomitant pathology. Research methods: clinical; laboratory (immunological - determination of metalloproteinase-12; genetic - polymorphism of the MMP12-82A>G gene); instrumental (computed tomography). The studies were carried out upon admission to the hospital. Statistical processing of the study results was carried out using the IBM SPSS Statistics Version 25.0 software package (IBM, USA). Results. A predominance of the -82A- allele of the MMP12-82(A>G) gene was recorded in the group with ARI and -82A/G genotype in patients with community-acquired pneumonia against the background of COVID-19 infection, while in severe cases a predominance of the -82A- allele and - was noted 82A/A genotype. An increase in the concentration of MMP-12 in the main group was found to be 3.6 times compared to the same value in the control group and 1.7 times compared to the group of community-acquired pneumonia against the background of ARI. Studying the effect of SNP MMP12-82A>G on the expression of MMP-12, a tendency was noted for its content to decrease in carriers of the -82G/G genotype.Conclusion. The -82A- allele and the -82A/A genotype of the MMP12 gene are associated with a severe course of community-acquired pneumonia (COVID-19, ARI) in patients of military age, while carriage of the -82Gallele and -82G/G genotype can be considered as a protective effect of a favorable course pathological process in the lungs. In patients of military age with community-acquired pneumonia, an increase in the concentration of MMP-12 is recorded, with the highest values in the group with severe pneumonia due to COVID-19 infection. When carrying the -82G/G genotype of the MMP12 gene, a decrease in the concentration of MMP-12 in the blood serum is recorded.