Role of Methanogenic and Sulfate-Reducing Bacteria in the Reductive Dechlorination of Tetrachloroethylene in Mixed Culture

Cabirol, Nathalie; Perrier, Joseph Louis; Jacob, François; Fouillet, Bruno; Chambon, Paul

doi:10.1007/s001289900119

Cited by 8 publications

(11 citation statements)

References 12 publications

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“…A methanogenic and sulfate-reducing mixed culture was obtained from anaerobic digested sludge from the Bourg-en-Bresse wastewater treatment unit. After several subcultures, the enrichment was conducted by using mineral salt medium, which contained 300 mM methanol and different concentrations of PCE (Cabirol et al, 1996(Cabirol et al, , 1998. A PCE-acclimated consortium was maintained in a 1-L semicontinuous anaerobic reactor operated at 37°C.…”

Section: Microorganismsmentioning

confidence: 99%

“…This antibiotic was an inhibitor of sulfate-reducing bacteria as previously demonstrated (Cabirol et al, 1996). At daily incubation periods, triplicates of active and control vials were used for analysis as previously described (Cabirol et al, 1996).…”

Section: ϫmentioning

confidence: 99%

“…After 1 month of incubation, methane formation was determined by gas chromatography. MPN determination of SRB was conducted with specific medium of these bacteria as previously described (Cabirol et al, 1996).…”

Section: ϫmentioning

confidence: 99%

“…Initially, high concentrations of PCE were dechlorinated in trichloroethylene (TCE) by methanogenic fermentation in batch conditions. The sulfate-reducing bacteria were not involved in PCE dehalogenation (Cabirol et al, 1996). This methanogenic and sulfate-reducing consortium was then cultivated continually in the presence of high concentrations of PCE.…”

mentioning

confidence: 99%

“…The amounts of chlorinated compounds (PCE, TCE) were determined by the direct injection of samples into a Perkin Elmer model Sigma 2000 gas chromatograph using a previously described procedure (Cabirol et al, 1996). The amounts of CH 4 and CO 2 were determined by a Girdel 330 gas chromatographic analysis by using a previously described procedure (Cabirol et al, 1996).…”

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confidence: 99%

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Interaction between methanogenic and sulfate-reducing microorganisms during dechlorination of a high concentration of tetrachloroethylene.

Cabirol

Jacob

Perrier

et al. 1998

J. Gen. Appl. Microbiol.

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Tetrachloroethylene (PCE) is a chlorinated aliphatic compound essentially used as a degreasing and drycleaning solvent. It is commonly found as a groundwater contaminant and because of its carcinogenic properties is considered a pollutant. Biotransformation of PCE in ethane or ethylene has been studied by many researchers at low concentrations under strict anaerobic conditions (De Bruin et al., 1992;Freedman and Gossett, 1989).The dechlorination process was observed in methanogenic, acetogenic, or sulfate-reducing habitats (Holliger and Schraa, 1994;Mohn and Tiedje, 1992). In anaerobic PCE-enrichment culture, methanogenesis and acetogenesis disappeared with high concentrations of PCE (Maymo-Gatell et al., 1995). Ecological understanding of such communities will probably be important in using dehalogenation in bioremediation processes (Mohn and Tiedje, 1992).A methanogenic and sulfate-reducing consortium degrading PCE was obtained in our laboratory from anaerobic-digested sludge from a wastewater treatment plant (Bourg-en-Bresse, France). Initially, high concentrations of PCE were dechlorinated in trichloroethylene (TCE) by methanogenic fermentation in batch conditions. The sulfate-reducing bacteria were not involved in PCE dehalogenation (Cabirol et al., 1996). This methanogenic and sulfate-reducing consortium was then cultivated continually in the presence of high concentrations of PCE. The methanogenic bacteria always had a deciding role in the dechlorination process after this enrichment period. The pollutant was completely degraded to carbon biomass and CO 2 . PCE dehalogenation has been studied in a semicontinuous fixed-bed reactor, revealing an attractive method for removing high concentrations of PCE in bioremediation processes (Cabirol et al., 1998 A methanogenic and sulfate-reducing consortium, which was enriched on medium containing tetrachloroethylene (PCE), had the ability to dechlorinate high concentrations of PCE. Dehalogenation was due to the direct activity of methanogens. However, interactions between methanogenic and sulfate-reducing bacteria involved modification of the dechlorination process according to culture conditions. In the absence of sulfate, the relative percentage of electrons used in PCE dehalogenation increased after an addition of lactate in batch conditions. The sulfate reducers would produce further reductant from lactate catabolism. This reductant might be used by methanogenic bacteria in PCE dechlorination. A mutualistic interaction was observed in the absence of sulfate. However in the presence of sulfate, methanogenesis and dechlorination decreased because of interspecific competition, probably between the H 2 -oxydizing methanogenic and sulfate-reducing bacteria in batch conditions. In the semicontinuous fixed-bed reactor, the presence of sulfate did not affect dechlorination and methanogenesis. The sulfate-reducing bacteria may not be competitors of H 2 -consuming methanogens in the reactor because of the existence of microbial biofilm. The presence of the fixed film may ...

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Section: Microorganismsmentioning

confidence: 99%

Section: ϫmentioning

confidence: 99%

Section: ϫmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Interaction between methanogenic and sulfate-reducing microorganisms during dechlorination of a high concentration of tetrachloroethylene.

Cabirol

Jacob

Perrier

et al. 1998

J. Gen. Appl. Microbiol.

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Degradation of perchloroethylene and dichlorophenol by pulsed-electric discharge and bioremediation

Yee

Chauhan

Yankelevich

et al. 1998

Biotechnol. Bioeng.

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Pulsed electric discharge (PED) and bioremediation were combined to create a novel two-stage system which dechlorinates the halogenated pollutants, 2,4dichlorophenol and perchloroethylene, with repetitive (0.1-1 kHz), short pulse (∼100 ns), low voltage (40-80 kV) discharges and then mineralizes the less chlorinated products with aerobic bacteria. A 6.1 mM aqueous dichlorophenol sample was cycled through the PED reactor (60 kV of applied pulsed voltage and 300 Hz) 6 times, resulting in the release of 55% of the initial dichlorophenol chloride ions (1 mM Cl − removed each cycle). The respective average specific efficiency is 0.4-0.6 keV/(Cl − molecule). Pseudomonas mendocina KR1, which grows in minimal medium supplemented with phenol but not with dichlorophenol, increased in cell density in all cultures supplemented with the PED-treated DCP samples and yielded a maximum of twofold additional Cl − released compared to the PED-related alone. The number of PED-treatment cycles, voltage, and frequency were also varied, showing that both cell densities and overall dichlorophenol dechlorination were highly dependent upon the number of PED-treatment cycles, rather than the tested voltages and frequencies. Using this two-stage treatment system, PED released 31% of the initial chloride ions from dichlorophenol (after three cycles at 40-45 kV and 1.2 kHz) while P. mendocina KR1 in the second stage increased dechlorination to 90%. These results were corroborated by the 35% additional chloride release found with activated sludge cultures. Perchloroethylene (0.6 mM) was similarly treated in a first-stage PED reactor (80% chloride removal after four cycles) followed by biodegradation of the dechlorinated products with a recombinant toluene o-monooxygenase-expressing Pseudomonas fluorescens strain. Gas chromatographic analysis showed that the PED reactor created less-chlorinated byproducts (i.e., trichloroethylene) that were removed (74%) upon exposure to the recombinant bacterium.

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Low and high acetate amendments are equally as effective at promoting complete dechlorination of trichloroethylene (TCE)

Wei

Finneran

2012

Biodegradation

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Experiments with trichloroethylene-contaminated aquifer material demonstrated that TCE, cis-DCE, and VC were completely degraded with concurrent Fe(III) or Fe(III) and sulfate reduction when acetate was amended at stoichiometric concentration; competing TEAPs did not inhibit ethene production. Adding 10× more acetate did not increase the rate or extent of TCE reduction, but only increased methane production. Enrichment cultures demonstrated that ~90 μM TCE or ~22 μM VC was degraded primarily to ethene within 20 days with concurrent Fe(III) or Fe(III) + sulfate reduction. The dechlorination rates were comparable between the low and high acetate concentrations (0.36 vs 0.34 day(-1), respectively), with a slightly slower rate in the 10× acetate amended incubations. Methane accumulated to 13.5 (±0.5) μmol/tube in the TCE-degrading incubations with 10× acetate, and only 1.4 (±0.1) μmol/tube with low acetate concentration. Methane accumulated to 16 (±1.5) μmol/tube in VC-degrading enrichment with 10× acetate and 2 (±0.1) μmol/tube with stoichiometric acetate. The estimated fraction of electrons distributed to methanogenesis increased substantially when excessive acetate was added. Quantitative PCR analysis indicated that 10× acetate did not enhance Dehalococcoides biomass but rather increased the methanogen abundance by nearly one order of magnitude compared to that with stoichiometric acetate. The data suggest that adding low levels of substrate may be equally if not more effective as high concentrations, without producing excessive methane. This has implications for field remediation efforts, in that adding excess electron donor may not benefit the reactions of interest, which in turn will increase treatment costs without direct benefit to the stakeholders.

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Role of Methanogenic and Sulfate-Reducing Bacteria in the Reductive Dechlorination of Tetrachloroethylene in Mixed Culture

Cited by 8 publications

References 12 publications

Interaction between methanogenic and sulfate-reducing microorganisms during dechlorination of a high concentration of tetrachloroethylene.

Interaction between methanogenic and sulfate-reducing microorganisms during dechlorination of a high concentration of tetrachloroethylene.

Degradation of perchloroethylene and dichlorophenol by pulsed-electric discharge and bioremediation

Low and high acetate amendments are equally as effective at promoting complete dechlorination of trichloroethylene (TCE)

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