Role of Methionine in Biosynthesis of Prodigiosin by<i>Serratia marcescens</i>

Qadri, S. M. Hussain; Williams, Robert P.

doi:10.1128/jb.116.3.1191-1198.1973

Cited by 20 publications

(8 citation statements)

References 21 publications

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“…We previously analyzed ( Kurbanoglu and Kurbanoglu, 2004 ) the chemical composition of RHP and determined that it is very rich in both organic and inorganic nutrients and contains many amino acids and inorganic materials (data are not given). Others have reported that some amino acids (alanine, serine, histidine, cystine, methionine, glutamate, tryptophan and proline) contribute to the formation of prodigiosin ( Williams et al , 1971 ; Qadri and Williams, 1973 ; Witney et al , 1977 ). The results of our previous studies ( Kurbanoglu and Kurbanoglu, 2004 ; Kurbanoglu 2004b ; Kurbanoglu and Kurbanoglu, 2007 ) showed that RHP contains all of these amino acids at varying concentrations and is especially rich in alanine, cysteine, glutamate and proline.…”

Section: Resultsmentioning

confidence: 99%

Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone

Kurbanoğlu¹,

Özdal²,

Ozdal³

et al. 2015

Braz. J. Microbiol.

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This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA classified the microbe as Serratia marcescens. The substrate utilization potential (BIOLOG) and fatty acid methyl ester profile (FAME) of S. marcescens were also determined. The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin. A concentration of 0.4% (w/v) RHP resulted in the greatest yield of prodigiosin (277.74 mg/L) after 48 h when mannitol was used as the sole source of carbon. The pigment yield was also influenced by the types of carbon sources and peptones. As a result, RHP was demonstrated to be a suitable substrate for prodigiosin production. These results revealed that prodigiosin could be produced efficiently by S. marcescens using RHP.

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Section: Resultsmentioning

confidence: 99%

Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone

Kurbanoğlu¹,

Özdal²,

Ozdal³

et al. 2015

Braz. J. Microbiol.

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“…Such a study was made possible by addition of proline to non-proliferating suspensions of strain Nima, the Aut mutant, and the Hut mutant, and of alanine to nonproliferating suspensions of the Put mutant, to induce metabolism and pigmentation ( Table 5). L-Methionine was added to these suspensions to increase the quantity of pigment formed (9). Once pigment was formed, labeled alanine, histidine, or proline was added to the cell suspensions, and the incorporation of these amino acids into the pigment was followed.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis of prodigiosin by non-proliferating wild-type Serratia marcescens and mutants deficient in catabolism of alanine, histidine, and proline

Lim¹,

Qadri²,

Nichols³

et al. 1977

J Bacteriol

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Mutants ofSerratia marcescens Nima, designated as Aut, Hut, or Put, did not utilize L-alanine, L-histidine, or L-proline, respectively, as a sole carbon source but did utilize other amino acids or glycerol as carbon sources. The bacteria were permeable to alanine, histidine, and proline but lacked the enzymes responsible for degradation of these amino acids. The Aut mutant contained no L-alanine dehydrogenase activity, whereas the Hut and Put mutants contained only 7 and 4% of the histidase and proline oxidase activities, respectively, found in the wildtype strain. Rates of oxygen uptake and protein synthesis were significantly lower when the mutants were incubated in the presence of amino acids they could not degrade. Studies of i_['4C]alanine, L-[14C]histidine, and L-['4C]proline

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“…After 12 h of incubation, radioactive proline was added to the cell suspension. At 16 h of incubation, prodigiosin was extracted from the cells, purified, and counted for radioactivity (12).…”

Section: Resultsmentioning

confidence: 99%

Incorporation of proline into prodigiosin by a Put mutant of Serratia marcesens

Lim¹,

Qadri²,

Williams³

1976

Appl Environ Microbiol

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A Put mutant of Serratia marcescens, deficient in proline oxidase and therefore unable to degrade proline, was used to assay for an enzymatic reaction responsible for incorporation of proline into prodigiosin. The reaction had a pH optimum of 7.5 and a Km of 1.1 x 10-4 M at 27 C. At temperatures above 27 C, the velocity of the reaction decreased with increasing temperature and little activity was detected at 42 C. Activity of the enzyme was directly proportional to the quantity of pigment formed and was inhibited by thioproline, a substrate analog. These data suggested the presence of a unique and specific enzyme in the biosynthetic pathway for prodigiosin.

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Role of Methionine in Biosynthesis of Prodigiosin bySerratia marcescens

Cited by 20 publications

References 21 publications

Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone

Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone

Biosynthesis of prodigiosin by non-proliferating wild-type Serratia marcescens and mutants deficient in catabolism of alanine, histidine, and proline

Incorporation of proline into prodigiosin by a Put mutant of Serratia marcesens

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