Role of Methionine Sulfoxide Reductases A and B of
            <i>Enterococcus faecalis</i>
            in Oxidative Stress and Virulence

Zhao, Chen; Hartke, Axel; Sorda, Marilena La; Posteraro, Brunella; Laplace, Jean-Marie; Auffray, Yanick; Sanguinetti, Maurizio

doi:10.1128/iai.00165-10

Cited by 88 publications

(87 citation statements)

References 39 publications

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“…The E. faecalis V19 parental strain (Table 1) used in this study is a plasmid-cured derivative of the vancomycin-resistant clinical isolate V583 (38). E. faecalis V19 and its derivates were cultivated at 37°C in M17 medium (36) supplemented with 0.5% glucose (GM17) or in M17 MOPS (morpholinepropanesulfonic acid) medium supplemented with glycerol as the sole carbohydrate (17).…”

Section: Methodsmentioning

confidence: 99%

Involvement of Peptidylprolyl cis / trans Isomerases in Enterococcus faecalis Virulence

et al. 2012

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ABSTRACTPeptidylprolylcis/transisomerases (PPIases) are enzymes involved in protein folding. Analysis of the genome sequence ofEnterococcus faecalisV583 allowed for identification of 3 PPIases carrying genes.ef2898encodes an intracellular PPIase which was not shown to be important for theE. faecalisstress response or virulence. The other two PPIases, the parvulin family rotamase EF0685 and the cyclophilin family member EF1534, are expected to be surface-exposed proteins. They were shown to be important for virulence and resistance to NaCl. A Δef0685Δef1534mutant was also more resistant to oxidative stress, was able to grow under a high manganese concentration, and showed altered resistance to ampicillin and quinolone antibiotics.

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Section: Methodsmentioning

confidence: 99%

Involvement of Peptidylprolyl cis / trans Isomerases in Enterococcus faecalis Virulence

et al. 2012

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“…The parental E. faecalis strain used in this work was strain V19, a plasmid-cured derivative of the vancomycin-resistant clinical isolate V583 (38). E. coli XLIBlue was used as the recipient for cloning.…”

Section: Methodsmentioning

confidence: 99%

SlyA Is a Transcriptional Regulator Involved in the Virulence of Enterococcus faecalis

et al. 2011

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Phylogenetic analysis of the crystal structure of the Enterococcus faecalis SlyA (EF_3002) transcriptional factor places it between the SlyA and MarR regulator subfamilies. Proteins of these families are often involved in the regulation of genes important for bacterial virulence and stress response. To gather evidence for the role of this putative regulator in E. faecalis biology, we dissected the genetic organization of the slyA-EF_3001 locus and constructed a slyA deletion mutant as well as complemented strains. Interestingly, compared to the wild-type parent, the ⌬slyA mutant is more virulent in an insect infection model (Galleria mellonella), exhibits increased persistence in mouse kidneys and liver, and survives better inside peritoneal macrophages. In order to identify a possible SlyA regulon, global microarray transcriptional analysis was performed. This study revealed that the slyA-EF_3001 locus appears to be autoregulated and that 117 genes were differentially regulated in the ⌬slyA mutant. In the mutant strain, 111 were underexpressed and 6 overexpressed, indicating that SlyA functions mainly as an activator of transcription.

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“…All three are involved in resistance to H 2 O 2 , to various degrees, while survival in murine macrophages was mostly affected in the ⌬tpx mutant and the peroxidase triple mutant. Two additional antioxidant repair enzymes, the methionine sulfoxide reductases MsrA and MsrB, have been characterized for E. faecalis (526). Mutants carrying a deletion in the respective genes exhibited sensitivity to H 2 O 2 and attenuated virulence in systemic and urinary tract infection models.…”

Section: Oxidative Stress and Virulencementioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

539

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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Role of Methionine Sulfoxide Reductases A and B of Enterococcus faecalis in Oxidative Stress and Virulence

Cited by 88 publications

References 39 publications

Involvement of Peptidylprolyl cis / trans Isomerases in Enterococcus faecalis Virulence

Involvement of Peptidylprolyl cis / trans Isomerases in Enterococcus faecalis Virulence

SlyA Is a Transcriptional Regulator Involved in the Virulence of Enterococcus faecalis

Stress Physiology of Lactic Acid Bacteria

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