Role of Microtubules in Lipoprotein Secretion by the Liver

Orci, Lelio; Marchand, Y. Le; Singh, Amreek; Assimacopoulos-Jeannet, F.; Rouiller, Ch.; Jeanrenaud, B.

doi:10.1038/244030a0

Cited by 143 publications

(30 citation statements)

References 13 publications

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“…Previous studies carried out either in an isolated system, the perfused livers of normal mice (1)(2)(3), or in vivo using normal rats_ (4) have shown independently that an intact microtubular system was necessary for the release of very low density lipoproteins (VLDL) by the liver. The experiments performed with the perfused mouse livers demonstrated in particular that the presence of colchicine or vincristine, drugs known to interfere with the microtubular system (5,6), resulted in a marked decrease in the secretion of VLDL into the perfusate and an accumulation of triglycerides in the liver at a time when electron microscopy revealed that microtubules had virtually disappeared from the hepatocytes and VLDL-like particles had accumulated within the parenchyma (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…The experiments performed with the perfused mouse livers demonstrated in particular that the presence of colchicine or vincristine, drugs known to interfere with the microtubular system (5,6), resulted in a marked decrease in the secretion of VLDL into the perfusate and an accumulation of triglycerides in the liver at a time when electron microscopy revealed that microtubules had virtually disappeared from the hepatocytes and VLDL-like particles had accumulated within the parenchyma (2,3). This suggested an inhibition of the intracellular translocation of VLDL particles.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for a Role of the Microtubular System in the Secretion of Newly Synthesized Albumin and Other Proteins by the Liver

Marchand¹,

Patzelt²,

Assimacopoulos-Jeannet³

et al. 1974

J. Clin. Invest.

110

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A B S T R A C T Livers of normal mice were prefused in situ and the secretion of newly synthesized (i.e. labeled) proteins into the perfusate were measured. In control livers, the secretion of newly synthesized proteins was found to be linear with time. In marked contrast, when livers were perfused with vinblastine, vincristine, or colchicine, drugs known to interfere with the hepatic microtubular system, the release of newly synthesized proteins was either strongly inhibited or completely suppressed although total hepatic protein synthesis (estimated by the incorporation of labeled amino acids into hepatic plus perfusate proteins) remained unaltered. Chromatographic separation of the various secreted proteins showed that the release of albumin, globulins, and small polypeptides was decreased to a similar extent by vincristine or colchicine. In the particular case of albumin, it was further observed that total (i.e. liver plus perfusate) labeled amino acid incorporation into albumin was not altered by either vincristine or colchicine, whereas the incorporation of these amino acids into liver albumin was markedly increased but incorporation into perfusate albumin was decreased, suggesting that the translocation of this particular protein from the liver to the perfusate had been affected by the presence of these drugs. It is proposed that the functional integrity of microtubules is necessary for the intracellular movement and eventual release of albumin and other proteins by the liver, and suggested that microtubules might possibly be a site of regulation of hepatic protein secretion.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence for a Role of the Microtubular System in the Secretion of Newly Synthesized Albumin and Other Proteins by the Liver

Marchand¹,

Patzelt²,

Assimacopoulos-Jeannet³

et al. 1974

J. Clin. Invest.

110

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show abstract

“…Microtubules have an important function in the intracellular translocation of cytoplasmic organdíes (Malawista, 1968 ; Gee & Cross, 1973 ;Orci et al, 1973). An expression of this function can be seen in many secretory processes in which densely staining granules become associated with microtubules before being transferred to the cell periphery where the granules are secreted by exocytosis.…”

mentioning

confidence: 99%

Effects of colchicine on the ovine corpus luteum: role of microtubules in the secretion of progesterone

Gemmell¹,

Stacy²

1977

Reproduction

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“…They were composed of thin straight parallel strands spaced at an average distance of 360 ~ on longitudinal sections and exhibited a honeycomb-like structure on cross sec-K. Kovacs et al (BENSCH and MALAWISTA, 1969;TYSON and BULGER, 1973). In the liver, disruption and disappearance of microtubules as well as the formation of paracrystalline inclusions have been reported in the cytoplasm of hepatocytes, sinusoidal endothelium and Kupffer cells (ORCI et al~ 1973), but to our knowledge the present work is the first in which this process has been revealed in the perisinusoidal cells.…”

Section: Resultsmentioning

confidence: 95%

Vinblastine-induced ultrastructural changes in perisinusoidal cells of the rat liver

et al. 1975

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Role of Microtubules in Lipoprotein Secretion by the Liver

Cited by 143 publications

References 13 publications

Evidence for a Role of the Microtubular System in the Secretion of Newly Synthesized Albumin and Other Proteins by the Liver

Evidence for a Role of the Microtubular System in the Secretion of Newly Synthesized Albumin and Other Proteins by the Liver

Effects of colchicine on the ovine corpus luteum: role of microtubules in the secretion of progesterone

Vinblastine-induced ultrastructural changes in perisinusoidal cells of the rat liver

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