Role of milk fat globule-epidermal growth factor 8 in osteoimmunology

Sinningen, Kathrin; Thiele, Stefanie; Hofbauer, Lorenz C.; Rauner, Martina

doi:10.1038/bonekey.2016.52

Cited by 6 publications

(6 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wang et al ( 47 ) suggested that APOC3 may be a prognostic biomarker for hepatocellular carcinoma (HCC). Milk fat globule-epidermal growth factor 8 (MFGE8) is a lipophilic glycoprotein that is widely involved in cell interactions and plays an important role in inflammatory injury, fibrosis, tumor development, and other diseases ( 48 ). Tian et al ( 49 ) highlighted that MFGE8, as a COX-2-related gene, is involved in the regulation of tumor lung colonization in triple negative breast cancer (TNBC), and that silencing MFGE8 expression in TNBC cells significantly restored sensitivity to selective inhibitors of COX-2 both in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of plasma exosomes in patients with non-small cell lung cancer

Bao¹,

Huang²,

Lang³

et al. 2022

Transl Lung Cancer Res

View full text Add to dashboard Cite

Background: Currently, the prognosis of patients with non-small cell lung cancer (NSCLC) remains unsatisfactory. This current study evaluated the relationship between histology of NSCLC and protein expression of exosomes in the plasma from NSCLC patients, and furthermore investigate the impact of the exosome profile on the tumor, node, metastasis (TNM) classification.Methods: Plasma samples were collected from 26 NSCLC patients before surgery. The exosomes were extracted from the plasma and liquid chromatography-mass spectrometry (LC/MS) was used to evaluate the expression of the proteins in the exosomes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed using the Cytoscape 3.8.2 software. Multivariate logistic regression and receiver operating characteristic (ROC) curves were used to identify proteins which could effectively distinguish between lung adenocarcinoma and lung squamous cell carcinoma. The relationship between protein expression and the TNM stage was calculated using Spearman rank correlation. Results:The expression levels of ZSWIM9, FYB1, SERPINF1, C1orf68, MASP2, and IGHV3-72 were higher in patients with lung adenocarcinoma compared to patients with lung squamous cell carcinoma.MFGE8 was associated with the occurrence of squamous cell carcinoma. CORO1A was positively correlated with the TNM stage of the patients, and COL4A2 was negatively correlated with TNM stage. GO and KEGG analyses revealed that cholesterol metabolism was important in NSCLC development.Conclusions: Lung adenocarcinoma may be distinguished from squamous cell carcinoma by the molecular profile of exosomes in the plasma samples. And, proteomics analysis suggested that cholesterol metabolism may play an important role of cancer progress in NSCLC.

show abstract

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of plasma exosomes in patients with non-small cell lung cancer

Bao¹,

Huang²,

Lang³

et al. 2022

Transl Lung Cancer Res

View full text Add to dashboard Cite

show abstract

“…A previous study indicated that MFG-E8 plays an important role in the balance of bone metabolism, especially under pathological conditions. 10 Moreover, patients with rheumatoid arthritis reportedly have lower serum concentrations of MFG-E8 than do healthy controls, and MFG-E8 deficiency augments the severity of arthritis in mice. 11 Similar to previous findings, the present study demonstrated that the plasma MFG-E8 level was lower in patients with than without knee OA.…”

Section: Discussionmentioning

confidence: 99%

“…9 A previous study showed that MFG-E8 plays an important role in the balance of bone metabolism, especially under pathological conditions. 10 Patients with rheumatoid arthritis have a lower serum concentration of MFG-E8 than do healthy controls, and MFG-E8 deficiency augments the severity of arthritis in mice. 11 Moreover, the involvement of proinflammatory cytokines and chronic inflammatory processes in the pathophysiology of OA is generally accepted.…”

Section: Introductionmentioning

confidence: 99%

Synovial fluid and plasma levels of milk fat globule–epidermal growth factor 8 are inversely correlated with radiographic severity of knee osteoarthritis

et al. 2019

View full text Add to dashboard Cite

Objective Mounting evidence demonstrates that inflammation plays an important role in the pathogenesis of osteoarthritis (OA). Milk fat globule–epidermal growth factor 8 (MFG-E8) is an important glycoprotein that is involved in anti-inflammatory responses. The present study was performed to assess the MFG-E8 levels in plasma and synovial fluid and explore the association between radiographic severity and MFG-E8 levels in patients with knee OA. Methods This study involved 138 healthy controls and 142 patients with knee OA. The MFG-E8 levels in plasma and synovial fluid were evaluated by enzyme-linked immunosorbent assay. The Kellgren and Lawrence classification was used for OA grading. Results The plasma MFG-E8 level was significantly lower in patients with knee OA than in healthy controls. The synovial fluid MFG-E8 level was significantly lower than the plasma level in patients with knee OA. More importantly, the MFG-E8 levels in synovial fluid and plasma were significantly and inversely associated with radiographic severity among patients with knee OA. Conclusions These results demonstrate that the levels of MFG-E8 in synovial fluid and plasma are inversely correlated with the radiographic severity of knee OA.

show abstract

“…20,21 MFG-E8-deficient mice exhibited increased RANKL-induced osteoclastogenesis ex vivo. 22,23 Sinningen et al also reported that MFG-E8-deficient mice exhibited impaired bone formation and mineralization compared with wildtype animals. 24 It is vital to promote the recruitment of BMSCs in the microenvironment surrounding a fracture hematoma and to induce osteogenic differentiation of MSCs to facilitate bone healing.…”

Section: Introductionmentioning

confidence: 98%

MFG‐E8 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells through GSK3β/β‐catenin signaling pathway

et al. 2023

View full text Add to dashboard Cite

Abbreviations: ALP, alkaline phosphatase; ARS, Alizarin red staining; BMP, bone morphogenetic protein; BMSCs, bone marrow mesenchymal stem cells; BTE, bone tissue engineering.; BV/TV, trabecular bone volume per total volume; COL1A1, collagen type I a 1 chain; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; H&E, hematoxylin and eosin; MFG-E8, milk fat globule-epidermal growth factor 8; OCN, osteocalcin; (active βcatenin, non-phosphorylated (active) βcatenin; t-βcatenin, total βcatenin; RANKL, receptor activator of nuclear factor κB ligand; RUNX2, runt-related transcription factor 2; siRNA, small interfering RNA.

show abstract

Role of milk fat globule-epidermal growth factor 8 in osteoimmunology

Cited by 6 publications

References 55 publications

Proteomic analysis of plasma exosomes in patients with non-small cell lung cancer

Proteomic analysis of plasma exosomes in patients with non-small cell lung cancer

Synovial fluid and plasma levels of milk fat globule–epidermal growth factor 8 are inversely correlated with radiographic severity of knee osteoarthritis

MFG‐E8 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells through GSK3β/β‐catenin signaling pathway

Contact Info

Product

Resources

About