Role of miR-191/425 Cluster in Tumorigenesis and Diagnosis of Gastric Cancer

Abstract

Gastric cancer (GC) is among the most frequent types of cancer worldwide. Therefore, understanding the biology of GC tumorigenesis is important for appropriate diagnosis and patient surveillance. The miR-191/425 cluster has been reported to be overexpressed in various human cancers, but the tumorigenic role and clinical significance of miR-191/425 overexpression in gastric carcinogenesis is currently undefined. In this study, the expression of miR-191 and miR-425 in GC tissue and serum was assessed, and the relationship between miRNA expression and clinicopathological data was analyzed. We found that miR-191 and miR-425 were both significantly increased in human GC tissues relative to adjacent normal controls. In addition, miR-191 levels correlated with GC tumor stage and metastatic state. Furthermore, the level of serum miR-191 was significantly higher in the GC group than in the control group when using serum miR-16 as an endogenous control. Finally, inhibition of miR-191 or miR-425 in the GC cell lines HGC-27 not only reduced cell proliferation and cell cycle progression but also impaired cell migration and invasion. Taken together, our results revealed the oncogenic roles of miR-191 and miR-425 in gastric carcinogenesis, and indicated the potential use of serum miR-191 as a novel and stable biomarker for GC diagnosis.

Diagnostic value of circulating microRNAs for gastric cancer in Asian populations: a meta-analysis

“…6 was conducted to make sure that our findings were not driven by any single study. After excluding seven outlier studies [21,29,39], the I 2 for heterogeneity decreased both in sensitivity (from 84.92 to 78.74 %) and specificity (from 83.00 to 77.19 %). The overall results showed only minimal changes and were similar to the original ones.…”

miRNA-15a/16: As Tumor Suppressors and More

“…Because studies showed that circulating miRNAs often bind to carrier proteins or are wrapped in exosomes, they may be better protected from RNase and serve as better endogenous controls. miR-16 has been used in a variety of studies, such as studies on gastric cancer [45], adrenocortical tumors [46] and coronary artery disease [47].…”

microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo

“…The predesigned TaqMan ® MicroRNA assays included the primer sets and TaqMan ® probe that were used. The thermal cycling conditions used for PCR were as follows: 95˚C for 10 min, followed by 40 cycles at 95˚C for 15 sec and 60˚C for 60 sec (17,18). Small nuclear U6 single nucleotide RNA (Applied Biosystems Life Technologies) was used as an internal control, and the relative expression was calculated using the comparative threshold cycle method.…”