Role of miRNA in Lung Cancer-Potential Biomarkers and Therapies

Du, Xiaohong; Zhang, Jitai; Wang, Juping; Liu, Xiaoming; Ding, Feng

doi:10.2174/1381612823666170714150118

Cited by 65 publications

(45 citation statements)

References 144 publications

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“…Therefore, it is of great significance to find the factors related to the occurrence, development and metastasis of lung cancer in order to predict its survival and prognosis and provide possible means for treatment. For a long time, the research on lung cancer has mostly focused on the cancer cells themselves, mainly due to changes in gene level [ [18] , [19] – 20 ]. In contrast, there is little discussion about the chronic infection of pathogenic microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Clinical significance and prognostic value of Porphyromonas gingivalis infection in lung cancer

Liu

Yuan

Chen

et al. 2021

Translational Oncology

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Section: Discussionmentioning

confidence: 99%

Clinical significance and prognostic value of Porphyromonas gingivalis infection in lung cancer

Liu

Yuan

Chen

et al. 2021

Translational Oncology

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“…Research shows that miR-148a inhibits the migratory and invasive potential of cells through targeting and regulating Wnt1 expression (16). Moreover, miRNAs can also serve as early diagnostic molecular markers in LC, as well as therapeutic targets and treatment evaluation markers (6,17). miR-30a expression in plasma is significantly elevated in patients with early LC, so its detection may be a new non-invasive method for early screening and differential diagnosis of patients with NSCLC (18).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-155-5p promotes cell proliferation and invasion in lung squamous cell carcinoma through negative regulation of fibroblast growth factor 9 expression

Liu¹,

Mao²,

Zhu³

et al. 2021

J Thorac Dis

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Background: Non-small cell lung cancer (NSCLC) ranks first for mortality among all malignancies.Squamous cell carcinoma (SCC) is one of the main types of NSCLC. Previous studies have found that fibroblast growth factor 9 (FGF9) is closely related to lung SCC via different molecular regulatory mechanisms. This study aimed to explore the relationship between microRNA-155-5p (miR-155-5p) and FGF9 gene expression and their effects on the proliferation and invasion of lung SCC through experiments, in order to provide theoretical basis for overcoming this disease. Methods: Fluorescence quantitative polymerase chain reaction was employed for the detection miR-155-5p and FGF9 expression in lung SCC tissues (n=40) and the corresponding adjacent normal tissues. The expression of FGF9 in the cancerous and adjacent tissues was detected by western blot. Transwell assay used to verify the effect of miR-155-5p on FGF-induced invasion and migration. Finally, subcutaneous tumor formation experiments in nude mice were used to verify how miR-155-5p and FGF9 affect the proliferative ability of lung SCC cells. Results:The results of fluorescence quantitative PCR revealed that miR-155-5p and FGF9 were expressed at high and low levels, respectively, in lung SCC tissue samples relative to normal adjacent tissue samples. Western blot analysis of 6 lung SCC tissue samples revealed a significantly reduced level of FGF9.Correlation analysis uncovered that miR-155-5p and FGF9 share a significant negative correlation in lung SCC. At the messenger RNA and protein levels miR-155-5p could negatively regulate the expression of FGF9. Bioinformatics and dual luciferase reporter assay results confirmed FGF9 to be a downstream regulatory gene targeted by miR-155-5p. Our in vitro and in vivo results demonstrated that FGF9 overexpression exerted a significant inhibitory effect on miR-155-5p's ability to promote lung cancer cell growth, invasion, and proliferation. Conclusions:Our results show that miR-155-5p, as an oncogene, negative regulates FGF9 expression to promote SCC occurrence and development in the lungs.

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“…miRNA expression signature for lung cancer was characterized by previous studies (27,28). Recently, a number of individual studies reported the dysregulated miRNAs in NSCLC and the functional roles of the individual miRNAs were investigated (29–35).…”

Section: Discussionmentioning

confidence: 99%

miR‑505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT‑NFκB pathway in NSCLC cells

Tang

Sun

et al. 2018

Int J Mol Med

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MicroRNAs (miRNAs) are short non-coding RNAs, which generally regulate gene expression at the post-transcriptional level. Dysregulation of miRNAs has been reported in numerous cancer types, including lung cancer. In the present study, the role of miR-505 in non-small cell lung cancer (NSCLC) cells was investigated. miR-505 served a tumor suppressor role in NSCLC cells. By reverse transcriptase-quantitative polymerase chain reaction detection, it was demonstrated that miR-505 was downregulated in NSCLC tissues and cell lines, which is negatively associated with large tumor size, Tumor-Node-Metastasis stage and distant metastasis in patients with NSCLC. Functional studies revealed that miR-505 inhibited cell proliferation, migration, invasion and epithelial-mesenchymal transition progress in vitro and tumor growth in vivo. Mechanically, mitogen-activated protein kinase kinase kinase 3 (MAP3K3) was identified as a direct target of miR-505 by binding to its 3′untranslated region and demonstrated to mediate the tumor suppressor roles of miR-505 in NSCLC cells. The effect of miR-505 on the activation of AKT/nuclear factor-κB (NFκB) pathway, which was downstream targets of MAP3K3, was further analyzed by western blot analysis and immunofluorescence analyses. The data demonstrated the inhibition of the AKT/NFκB pathway upon overexpressing miR-505 and the activation of AKT/NFκB pathway upon silencing miR-505. Collectively, the data revealed the novel role and target of miR-505 in NSCLC cells, which may provide novel insights regarding its role in the carcinogenesis of NSCLC and its potential values for clinical applications.

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Role of miRNA in Lung Cancer-Potential Biomarkers and Therapies

Cited by 65 publications

References 144 publications

Clinical significance and prognostic value of Porphyromonas gingivalis infection in lung cancer

Clinical significance and prognostic value of Porphyromonas gingivalis infection in lung cancer

MicroRNA-155-5p promotes cell proliferation and invasion in lung squamous cell carcinoma through negative regulation of fibroblast growth factor 9 expression

miR‑505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT‑NFκB pathway in NSCLC cells

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