Role of mitotic inhibitors and genotype on chromosome doubling of Rosa

Khosravi, Pegah; Kermani, Maryam Jafarkhani; Nematzadeh, Ghorbanali; Bihamta, Mohammad Reza; Yokoya, K.

doi:10.1007/s10681-007-9571-7

Cited by 56 publications

(39 citation statements)

References 22 publications

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“…The authors also reported a reduction in plant growth from 0 to 45% (control and 10 mM) and 0 to 100% (control and 60 µM) in the colchicine and oryzalin treatments, respectively. Reduced survival of banana (Costa et al, 2011) and rose explants (Khosravi et al, 2008) was also observed with the use of oryzalin. In contrast, colchicine promotes a greater recovery rate of stable tetraploids in Eriobotrya japonica (Thunb.)…”

Section: Effect Of Oryzalin Concentration and Exposure Time On Cassavmentioning

confidence: 97%

“…These results suggest that there are different levels of sensitivity to colchicine depending on the cassava variety used in the treatments. Khosravi et al (2008) reported that the success in chromosomal doubling in rose depends on the genotype evaluated and the ploidy level of the species, where low ploidy levels were more prone to chromosomal doubling. According to these authors the application of 6 µM oryzalin for 24 h in Rose persica (2n = 2x), R. hybrida cv Iceberg (2n = 3x) and R. hybrida cv Akito (2n = 4x) resulted in 60.0, 6.3, and 0% of chromosomal doubling, respectively.…”

Section: Effect Of Colchicine On the Survival Of Cassava Explantsmentioning

confidence: 99%

“…Alternative antimitotics such as oryzalin have been used because of its low toxicity and high in vitro polyploidization activity. Moreover, this antimitotic can be used at lower concentrations but with similar or higher efficiency compared to colchicine (Kermani et al, 2003;Khosravi et al, 2008;Scagliusi et al, 2009;Quesenberry et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Inducing autotetraploids in cassava using oryzalin and colchicine and their in vitro morphophysiological effects

Carvalho

Gomes

Souza³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Polyploid induction has been used for plant breeding to produce bigger and more robust plants than diploid types. The present study aimed to develop a methodology for in vitro induction of polyploidy in cassava. Apical and lateral microcuttings from the BRS Formosa variety were treated with six oryzalin concentrations for 24 and 48 h. The same methodology was used for colchicine with different concentrations. After 45 days of cultivation and an additional 45 days of subculture, the viability of the explants was assessed and plant acclimatization was performed. Ploidy was determined using flow cytometry. Oryzalin dose and exposure negatively affected cassava explant growth and development compared to untreated explants. Furthermore, apical and lateral explants responded differently to the treatments, showing a diversity in antimitotic sensitivity and effect that is tissue-type specific. In contrast, the doses of 1.25 to 6.25 mM colchicine resulted in high mortality of cassava explants. Therefore, the type of antimitotic affects the morphophysiological behavior of cassava plants in vitro, although apical explants have higher viability and regenerative capacity compared to lateral explants. In addition, the lateral explants have lower mixoploid rates compared to apical explants. Of the 310 plants generated by oryzalin treatments, 277 were diploid, 31 were mixoploid, and 2 were tetraploid. Exposure to oryzalin led to low rate of tetraploids and colchicine caused phytotoxic reactions and death of the explants. The tetraploids were multiplied in vitro to evaluate their yield in the field as well as their behavior against abiotic and biotic stress.

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Section: Effect Of Oryzalin Concentration and Exposure Time On Cassavmentioning

confidence: 97%

Section: Effect Of Colchicine On the Survival Of Cassava Explantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inducing autotetraploids in cassava using oryzalin and colchicine and their in vitro morphophysiological effects

Carvalho

Gomes

Souza³

et al. 2016

Genet. Mol. Res.

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“…Praça et al (2009) also employed the shoot tip as a source of explants for in vitro polyploidization of Stupické with no death records for the distinct treatments. Khosravi et al (2008) reported that the efficiency of polyploidization depends on the type of explant and its interaction with the anti-mitotic agent. According to Roy et al (2001) and Praça et al (2009), colchicine is adequate for in vitro induction of polyploids.…”

Section: Nuclear 2c Value and Karyotype Of 'Santa Cruz Kada Gigante'mentioning

confidence: 99%

<i>In Vitro</i> Polyploidization in<i> Solanum lycopersicum</i> Mill. ‘Santa Cruz Kada Gigante'

et al. 2014

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Summary Solanum lycopersicum Mill. is a model plant for biological approaches and includes accessions with great agronomic relevance, such as the Santa Cruz Kada Gigante . For development of new S. lycopersicum accessions, in vitro tissue culture has proved relevant, contributing to the establishment of germplasm banks and breeding programs. In spite of the vast amount of tissue culture research already carried out in S. lycopersicum, in vitro polyploidization is still emerging. Thus, this study aimed to induce polyploid plantlets from Santa Cruz Kada Gigante from shoot tip explants. This accession presented 2n=2x=24 chromosomes and 2C=2.00 picograms of nuclear DNA. Hence, no preexisting variation was identified, and Santa Cruz Kada Gigante was considered appropriate for in vitro polyploidization. This procedure provided tetraploid (4x) and mixoploid (2x/4x and 2x/4x/8x) plantlets, which were identified by flow cytometry (FCM) and multiplied in vitro. Based on these results, the tissue culture procedure provided polyploid Santa Cruz Kada Gigante plantlets, which represent a new source of variability for breeding programs of S. lycopersicum.

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“…However, nowadays, colchicine is gradually substituted by other antimitotic agents due to its toxicity to humans by binding specifically to animal tubulin dimers. It was reported that antimicrotubule herbicides such as trifluralin are more specific for plant tubulin and mitosis in vitro compared to colchicine, and that these chemicals are at least as efficient as colchicine in chromosome doubling, but were less phytotoxic (Mu and Cao, 1999;Khosravi et al, 2008). However, for herbicides, the concentration is the key issue in their application.…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of tetraploid garlic with trifluralin

Cheng

Zhou²,

Khan³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Garlic (Allium sativum) is propagated asexually. Since sexual cross breeding is almost impossible, means for effective breeding are not currently available and the available production cultivars are seriously aged and degenerated. A possible alternative for breeding is chemical induction. Trifluralin, a type of herbicide, has been reported to provoke chromosome doubling. However, this chemical had not been tested on garlic. We tested various trifluralin concentrations and treatment durations for efficiency in the induction of tetraploid garlic. A clove base of garlic with a stem cv. Gailiang was used as the ex-plant to induce calluses on Murashige and Skoog (MS) medium; the calluses were then inoculated onto MS medium containing different levels of trifluralin and cultured to induce chromosome number variation in vitro. Garlic calluses were effectively induced via the ex-plant and both shoots and roots differentiated well on MS medium containing 6-benzylaminopurine at 3.0 mg/L and indole-3-acetic acid at 0.1 mg/L. However, increases in trifluralin concentration and treatment duration reduced the survival rate and differentiation rate of calluses. Garlic callus cultured for 15 days on medium containing 100 μM trifluralin gave the highest rate of chromosome doubling. Through observation of chromosome number in the root apical cells and the morphology ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (3): 2620-2628(2012 Induction of tetraploid garlic with trifluralin 2621 of guard cells on the leaf epidermis of the regenerated plantlets, it was clear that chromosome number variation was induced and tetraploids were produced in vitro by trifluralin treatment.

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Role of mitotic inhibitors and genotype on chromosome doubling of Rosa

Cited by 56 publications

References 22 publications

Inducing autotetraploids in cassava using oryzalin and colchicine and their in vitro morphophysiological effects

Inducing autotetraploids in cassava using oryzalin and colchicine and their in vitro morphophysiological effects

<i>In Vitro</i> Polyploidization in<i> Solanum lycopersicum</i> Mill. ‘Santa Cruz Kada Gigante'

In vitro induction of tetraploid garlic with trifluralin

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