Role of modulatory mediator serotonin in induction of epigenetic processes during long-term memory formation in Helix

Grinkevich, L. N.; Vorobiova, O. V.

doi:10.1134/s2079059714060094

Cited by 9 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mir-29b is downregulated in hippocampus during fear memory formation, which leads to changes in the DNA methylation and changes in the expression of the genes involved in plasticity [77], and Mir-29a regulates the serotonin receptor 5-HT7 expression [87]. As noted above, serotonin plays an important role in the formation of the food aversion reflex in Helix [30,32,38]. In addition, Mir-29 is able to modulate synapse morphology and memory processes via the p38 MAPK signaling pathway [88].…”

Section: Discussionmentioning

confidence: 95%

“…Suppression of the serotoninergic system leads to a deterioration in the formation of long-term forms of conditioned defensive reflexes [29,36,37]. We have shown that several transcription factors (TF) and epigenetic modifications of histones are involved in the conditioned aversive reflex formation and these processes are induced by serotonin through activation of the MAPK/ERK regulatory cascade [30][31][32]38,39]. These epigenetic modifications are under the control of both the activation and inhibition pathways involved in the formation of LTM.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum

Vasiliev

Ovchinnikov

Lisachev

et al. 2022

IJMS

View full text Add to dashboard Cite

Mollusks are unique animals with a relatively simple central nervous system (CNS) containing giant neurons with identified functions. With such simple CNS, mollusks yet display sufficiently complex behavior, thus ideal for various studies of behavioral processes, including long-term memory (LTM) formation. For our research, we use the formation of the fear avoidance reflex in the terrestrial mollusk Helix lucorum as a learning model. We have shown previously that LTM formation in Helix requires epigenetic modifications of histones leading to both activation and inactivation of the specific genes. It is known that microRNAs (miRNAs) negatively regulate the expression of genes; however, the role of miRNAs in behavioral regulation has been poorly investigated. Currently, there is no miRNAs sequencing data being published on Helix lucorum, which makes it impossible to investigate the role of miRNAs in the memory formation of this mollusk. In this study, we have performed sequencing and comparative bioinformatics analysis of the miRNAs from the CNS of Helix lucorum. We have identified 95 different microRNAs, including microRNAs belonging to the MIR-9, MIR-10, MIR-22, MIR-124, MIR-137, and MIR-153 families, known to be involved in various CNS processes of vertebrates and other species, particularly, in the fear behavior and LTM. We have shown that in the CNS of Helix lucorum MIR-10 family (26 miRNAs) is the most representative one, including Hlu-Mir-10-S5-5p and Hlu-Mir-10-S9-5p as top hits. Moreover, we have shown the involvement of the MIR-10 family in LTM formation in Helix. The expression of 17 representatives of MIR-10 differentially changes during different periods of LTM consolidation in the CNS of Helix. In addition, using comparative analysis of microRNA expression upon learning in normal snails and snails with deficient learning abilities with dysfunction of the serotonergic system, we identified a number of microRNAs from several families, including MIR-10, which expression changes only in normal animals. The obtained data can be used for further fundamental and applied behavioral research.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum

Vasiliev

Ovchinnikov

Lisachev

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…It participates in a number of important physiological functions [81,82]. The participation of the serotonin system in animal cognition and behavior, in learning and memory attracts increasing attention [28,[83][84][85]. Therefore, the deregulation of the serotonergic system causes the pathogenesis of many psychiatric and neurological disorders [84,86].…”

Section: Discussionmentioning

confidence: 99%

Effects of Thryptophan Hydroxylase Blockade by P-Chlorophenylalanine on Contextual Memory Reconsolidation after Training of Different Intensity

Deryabina

Андрианов

Муранова

et al. 2020

IJMS

View full text Add to dashboard Cite

The processes of memory formation and its storage are extremely dynamic. Therefore, the determination of the nature and temporal evolution of the changes that underlie the molecular mechanisms of retrieval and cause reconsolidation of memory is the key to understanding memory formation. Retrieval induces the plasticity, which may result in reconsolidation of the original memory and needs critical molecular events to stabilize the memory or its extinction. 4-Chloro-DL-phenylalanine (P-chlorophenylalanine-PCPA) depresses the most limiting enzyme of serotonin synthesis the tryptophan hydroxylase. It is known that PCPA reduces the serotonin content in the brain up to 10 times in rats (see Methods). We hypothesized that the PCPA could behave the similar way in snails and could reduce the content of serotonin in snails. Therefore, we investigated the effect of PCPA injection on contextual memory reconsolidation using a protein synthesis blocker in snails after training according to two protocols of different intensities. The results obtained in training according to the first protocol using five electrical stimuli per day for 5 days showed that reminding the training environment against the background of injection of PCPA led to a significant decrease in contextual memory. At the same time, the results obtained in training according to the second protocol using three electrical stimuli per day for 5 days showed that reminding the training environment against the injection of PCPA did not result in a significant change in contextual memory. The obtain results allowed us to conclude that the mechanisms of processes developed during the reconsolidation of contextual memory after a reminding depend both on the intensity of learning and on the state of the serotonergic system.

show abstract

“…Через час после обучения в центральной нервной системе Helix мы наблюдали активацию регуляторного каскада MAPK/ERK и MAPK/ERK-зависимые эпигенетические модификации гистонов [12,14,22], что может свидетельствовать в пользу скоординированности индукции хроматиновых перестроек, экспрессии микроРНК и важной роли МАРК/ ERK в этих процессах. На основе параллельного полногеномного анализа модификаций гистонов и микроРНК в 2015 г. показано наличие согласованных действий микроРНК и модификаций гистонов в регуляции экспрессии генов у человека [23].…”

Section: Discussionunclassified

Influence of PLL treatment on the long-term memory formation in Helix mollusk

Grinkevich

Николаевна²

2020

Medical academic journal

View full text Add to dashboard Cite

Relevance. The studies of the epigenetic mechanisms of long-term memory formation (LTM) has attracted the attention of many world leading laboratories since gained knowledge can be applied to correct cognitive impairments. miRNA dependent suppression of gene expression is the most complicated step in the epigenetic regulation, associated with a huge number of miRNAs (tens of thousands) and the diversity of their targets, thus the knowledge of miRNAs functions during LTM is still very fragmented. Aim. The aim of this study was to investigate the involvement of miRNAs in the formation of long-term memory using the model of the food aversion conditioned reflex development in the mollusk Helix. Prevention of the formation of mature miRNAs via Poly-L-lysine hydrobromide (PLL) treatment - inhibitor of Dicer activity was used as the main approach. Materials and methods. PLL was injected into animals during training, or 1, 3 or 5 hours after training. Success of the formation of conditioned reflexes was tested 72 hours after training. Results. There was a significant deterioration in LTM in animals with injected PLL 1 and 3 hours after training procedure compared with trained animals that were not injected with PLL. The treatment with PLL during training, or 5 hours after training, had no effect on LTM. Conclusion. Treatment with PLL, inhibitor of miRNA biogenesis disrupts formation of the food aversion reflex in Helix. Thus, miNRAs are involved in the LTM formation on Helix. Impaired expression of miRNAs is critical for the long-term memory formation if occurs in the intervals of 1 to 3 hours after training. We can recommend PLL for the investigations in the area of the epigenetic mechanisms of long-term memory.

show abstract

Role of modulatory mediator serotonin in induction of epigenetic processes during long-term memory formation in Helix

Cited by 9 publications

References 30 publications

The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum

The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum

Effects of Thryptophan Hydroxylase Blockade by P-Chlorophenylalanine on Contextual Memory Reconsolidation after Training of Different Intensity

Influence of PLL treatment on the long-term memory formation in Helix mollusk

Contact Info

Product

Resources

About