Role of Moraxella catarrhalis outer membrane protein CD in bacterial cell morphology and autoaggregation

A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens

“…Finally, we present in detail an assay with a set of multiplexed antigens designed for the quantitation of antibodies against common respiratory tract pathogens, which could be easily modified for different studies and different protein antigens. Although there is no currently licensed vaccine using all the studied antigens, most of them are considered vaccine candidates and some are part of experimental vaccines (Adamou et al, 2001;Bologa et al, 2012;Briles et al, 2000;Brooks-Walter et al, 1999;Crain et al, 1990;Croney et al, 2012;Kamtchoua et al, 2013;Khan and Pichichero, 2012;Khan et al, 2012;Poolman et al, 2000;Principi and Esposito, 2011;Saito et al, 2013;Seiberling et al, 2012;Smidt et al, 2013;Tai, 2006). Therefore, this methodology can be used as a basis to create other protocols specific for each of these new potential vaccine combinations.…”

“…Further, it has been reported that protein D as a carrier in pneumococcal conjugate vaccine (PCV) provides protection against AOM caused by H. influenzae (De Wals et al, 2009). Several adhesins have also been identified in M. catarrhalis that would be suitable as diagnostic targets or as vaccine candidates, such as the outer membrane protein CD (OMP CD), an adhesin that also has other functions in pathogenesis, and Msp22, a surface lipoprotein (Murphy and Parameswaran, 2009;Saito et al, 2013;Smidt et al, 2013). Although there is no currently licensed vaccine using all the aforementioned proteins (except for the use of protein D as a carrier in one of the PCVs), these antigens merit further investigation.…”

Outer membrane protein CD of Moraxella bovis as a potential immunogen against infectious bovine keratoconjunctivitis

“…In particular, OmpCD has been shown to act as a protective antigen in M. catarrhalis infections. OmpCD is conserved in most M. catarrhalis strains and exhibits attractive properties as a vaccine candidate (Hsiao et al, 1995;Saito et al, 2013). This protein is surface-exposed and contains at least two epitopes that are present in all of the studied strains of M. catarrhalis (Sarwar et al, 1992).…”

“…This protein is surface-exposed and contains at least two epitopes that are present in all of the studied strains of M. catarrhalis (Sarwar et al, 1992). OmpCD also exhibits characteristics of a porin-like OMP, relevant in nutrient acquisition (Holm et al, 2004;Saito et al, 2013). It has been proposed that M. catarrhalis OmpCD serves as an adhesin during human lung cell attachment acquisition (Holm et al, 2004) and plays an essential role in adherence to HEp-2 cells, contributing to the colonization and infection of laryngeal cells (Saito et al, 2013).…”

“…OmpCD also exhibits characteristics of a porin-like OMP, relevant in nutrient acquisition (Holm et al, 2004;Saito et al, 2013). It has been proposed that M. catarrhalis OmpCD serves as an adhesin during human lung cell attachment acquisition (Holm et al, 2004) and plays an essential role in adherence to HEp-2 cells, contributing to the colonization and infection of laryngeal cells (Saito et al, 2013). Mucosal vaccination has several advantages over traditional systemic vaccination, being the most effective route to elicit a protective response in mucosal surfaces (Holmgren y Czerkinsky, 2005).…”