Role of Muscarinic M1, M2, and M3 Receptors in the Regulation of Electrical Activity of Myocardial Tissue of Caval Veins during the Early Postnatal Ontogeny

Ivanova, Angelina; Tapilina, S. V.; Kuzmin, Vladislav S.

doi:10.1007/s10517-019-04364-9

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…To induce the selective activation of M3 cholinoreceptors we have used the protocol with muscarinic agonist pilocarpine validated by several earlier studies ( Abramochkin et al, 2012 ; Wang et al, 2012 ; Filatova et al, 2017 ), although more selective and high-affine antagonists of M2 and M3 receptors were used. Muscarinic agonist pilocarpine (10 -5 M) with slight selectivity to M1 and M3 receptors was applied to the experimental chamber alone or in the presence of highly selective M2 antagonist AQ-RA 741, 10 -7 M ( Doods et al, 1991 , 1994 ; Ivanova et al, 2019 ) after prior exposure of preparations to AQ-RA 741 alone. M3 antagonist J-104129 with high selectivity for M3 over M2 receptors ( Mitsuya et al, 2000 ) was used in special control experiments to ensure that cholinergic effects remaining in the presence of AQ-RA 741 are attributed to M3 receptor activation.…”

Section: Methodsmentioning

confidence: 99%

The role of M3 receptors in regulation of electrical activity deteriorates in the rat heart during ageing

Tapilina

Ivanova

Филатова

et al. 2022

Current Research in Physiology

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Ageing is a complex process which affects all systems of the organism and therefore changes the environment where the heart is working. In this study we demonstrate the ageing-related changes in the mechanisms of parasympathetic regulation of mammalian heart. Electrophysiological effects produced by selective activation of M3-cholinoreceptors were compared in isolated cardiac preparations from young adult (4 months), adult (1 year) and ageing (2 years) rats using sharp glass microelectrode technique. M3-receptors were activated with muscarinic agonist pilocarpine (10 -5 M) in the presence of selective M2 antagonist AQ-RA741 (10 -7 M). In atrial and ventricular myocardium from young rats M3 stimulation induced shortening of action potentials(APs), while no significant effect was observed in both elder groups. The main mechanism of M3-induced AP shortening is inhibition of L-type Ca 2+ current, estimated using whole-cell patch-clamp. It was negligible in atrial myocytes from ageing animals in comparison with young rats. The loss of sensitivity to stimulation of M3-receptors is due to decrease in M3 gene expression, shown by RT-PCR both in atrial and ventricular samples from ageing rats. Thus, in ageing rat heart M3-receptors are down-regulated and not involved in regulation of electrical activity.

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Section: Methodsmentioning

confidence: 99%

The role of M3 receptors in regulation of electrical activity deteriorates in the rat heart during ageing

Tapilina

Ivanova

Филатова

et al. 2022

Current Research in Physiology

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“…This increase in adhesion rate due to Ang II was reduced in group 3 due to treatment with ARB. This suggests that Ang II promotes the development of an inflammatory response and intimal injury, while treatment with ARB can alleviate these effects and may promote reconstruction of the disrupted tissue by increasing the proportion of M2 cells (Ivanova et al, 2019).…”

Section: Ang II Promotes Macrophage M1 Polarization and Adhesion And Treatment With Arb Suppresses These Effectsmentioning

confidence: 99%

AT1R Regulates Macrophage Polarization Through YAP and Regulates Aortic Dissection Incidence

Wang

Zhang

et al. 2021

Front. Physiol.

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Aortic dissection (AD) is one of the most fatal cardiovascular emergency. At the anatomical level, AD occurs due to the formation of intimal tears. However, the molecular mechanism underlying this phenomenon remains unknown. Angiotensin II (Ang II) is a important effector in the development of cardiovascular disease that acts through binding to angiotensin type 1 receptor (AT1R). Yes-associated protein (YAP) was recently recognized as a key protein in macrophage activation. To determine whether AT1R and YAP are involved in macrophage-induced endothelial cell (EC) inflammation and AD incidence, we co-cultured THP-1 cells and HAECs in transwell chambers under different culture conditions and apply different conditions to the AD mice model. The results showed that Ang II promoted macrophage M1 polarization and adhesion, upregulated YAP phosphorylation, and induced EC injury that was related to increased levels of multiple pro-inflammatory chemokines. Blocking AT1R function pharmacologically or by transfection with AT1R siRNA can reduce the pro-inflammatory effect induced by Ang II. In addition, siRNA knock down of YAP expression further aggravated the pro-inflammatory effects of Ang II. Treatment with ARB effectively alleviated these pro-inflammatory effects. In the mice AD model, ARB effectively reduced the incidence of AD in mice, decreased M1 macrophages infiltration and AT1R content in the aortic wall and increased the tissue content of YAP. We found that AT1R induces YAP phosphorylation through binding to Ang II, and further promotes macrophage M1 polarization and adhesion to ECs. ARB reduces the incidence of AD in mice and affect macrophage polarization in mice aorta.

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Role of Muscarinic M1, M2, and M3 Receptors in the Regulation of Electrical Activity of Myocardial Tissue of Caval Veins during the Early Postnatal Ontogeny

Cited by 2 publications

References 14 publications

The role of M3 receptors in regulation of electrical activity deteriorates in the rat heart during ageing

The role of M3 receptors in regulation of electrical activity deteriorates in the rat heart during ageing

AT1R Regulates Macrophage Polarization Through YAP and Regulates Aortic Dissection Incidence

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