Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons

Abstract

Myosin-Va, widely distributed throughout the developing nervous system, is involved in the transport of vesicles and other intracellular components with its globular tail domain (GTD) implicated in cargo recognition/interaction. Inactivation of myosin-Va in dorsal root ganglia (DRG) neurons of chick embryos, in vitro, decreases the rate of filopodial extension. MYO5A mutant mice have severe neurological defects. We have found that the overexpression of GTD in DRG cultures reduces the number of neurons with long neurites (above fourfold cell body length) and increased the number of neurons with short or no neurites. However, if transfection occurred after the onset of neuritogenesis, this was not seen. In embryo, we characterized the expression pattern of myosin-Va during neuritogenesis of TrkA-positive cells at different stages of chick DRG development. Myosin-Va expression was detected starting from HH25. At this stage, it was present in cells both with and without neurites. The presence of myosin-Va in DRG neurites persisted throughout the last stage analysed (HH34). The data suggest that Myosin Va can participate in embryonic DRG neuritogenesis.

In vitro models for neurotoxicology research

“…Usually, primary cultures of sensory neurons are prepared from DRG or trigeminal ganglia of mouse or rat embryos shortly after sensory neurons are generated (approximately on E10-13 in mice 257,318 and E9-16 in rats 257,258,319,320 ), as well as from neonatal or adult animals. [321][322][323] Nevertheless, although sensory neurons from many different vertebrate species, such as frogs, 324 chicken 325 and even humans, 326 have been isolated and cultured, mice have a particular genetic advantage, given the many publicly available transgenic mouse lines in which genes have been deleted 257 or altered. 327 Embryonic and neonatal preparations have the advantage of higher cell yields and greater proportion of neurons.…”

Цитоморфология Спинномозговых Ганглиев Кур

“…Их состояние отражает процессы, протекающие в организме при возрастной и адаптационной изменчивости. Спинномозговые ганглии лабораторных животных и человека изучены довольно подробно на разных стадиях пре-и постнатального онтогенеза, а также при экспериментальных воздействиях [7,8]. Сведений о видовой специфике развития этих органов у кур недостаточно и эти исследования, главным образом, проводились на эмбрионах с помощью светового микроскопа [9,10,11,12,13,14,15]…”