Role of naive and memory T cells in tumor cell lysis mediated by Bi-specific antibodies

Renner, Christoph; Held, Gerhard; Ohnesorge, Sascha; Bauer, Stefan; Gerlach, Klaus; Pfitzenmeier, Jan-Peter; Pfreundschuh, Michael

doi:10.1016/s0171-2985(97)80062-9

Cited by 13 publications

(12 citation statements)

References 29 publications

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“…Their speci®c binding to the RP1 promoter was con®rmed by electromobility shift assays. The cDNA encoding RP1 was initially isolated from activated T-cells after e ective stimulation via combined CD3 and CD28 antigen crosslinking (Renner et al, 1997). The fact that the RP1 promoter is regulated by CREB1 and 2 provides a link back to the process of T-cell activation (Butscher et al, 1998;Hsueh et al, 1997;Feuerstein et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…Jurkat cells were used since RP1 RNA expression can be rapidly induced to high levels in this cell line after activation (Renner et al, 1997). The protein/DNA-interaction reached maximum levels after 4 h (lane 4) and 24 h (lane 6) and di erent protein/ DNA-complexes could be detected (Figure 7b).…”

Section: Characterization Of Protein/dna Interaction By Electromobilimentioning

confidence: 99%

“…All PCR reactions were performed as described (Renner et al, 1997) using standard PCR conditions: 958C 5 min, 948C 1 min, annealing 608C, 1 min, extension 728C 2 min for 35 cycles. PCR chemicals including high ®delity Taq were obtained from Boehringer Mannheim (Mannheim, Germany).…”

Section: Polymerase Chain Reaction (Pcr) and Synthetic Oligo-nucleotidesmentioning

confidence: 99%

“…DNA sequencing was performed according to standard procedures using automated DNA sequencing ((SequiTherm EXCELII Long-Read DNA Sequencing Kit-LC, Biozym, Oldenburg, Germany) Sequencing automat LICOR 4000L (MWG, Munich, Germany)). Nucleotide sequence data were analysed using public data bases as described (Renner et al, 1997).…”

Section: Isolation Of the Genomic Rp1 Clonementioning

confidence: 99%

“…Total RNA from resting and stimulated Jurkat cells was isolated with guanidinium isothiocyanat as described (Renner et al, 1997) and the primer extension analysis performed according to standard methods (Sambrook et al, 1989). In brief, 10 mg of total RNA was hybridized with an end labelled primer ((T4-Polynucleotidkinase, Promega, Mannheim) and gamma-32 -P-ATP) corresponding to the region 18 bp (+445) upstream of the translation start codon of the anti-sense strand of the human RP1-cDNA.…”

Section: Primer Extension Analysismentioning

confidence: 99%

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Chromosomal localization and promoter analysis of the adenomatous polyposis coli binding protein RP1

et al. 2001

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The EB1/RP1 family is a new protein family that is characterized by the ability of its members to serve as interacting partners for the adenomatous polyposis coli (APC) tumour suppressor protein and tubulin. Data obtained with highly conserved yeast homologues suggest that the EB1/RP1 protein family promotes cytoplasmic microtubule dynamics and contributes to the sensor mechanism controlling the cytokinesis checkpoint during mitosis. However, the precise function of this protein family in mammalian cells has not been elucidated so far and remains unclear. Here, we report on the genomic localization of the RP1 gene and the characterization of the corresponding promoter. The RP1 gene was found to be encoded on chromosome 18q21, a locus which is altered or deleted in up to 50% of all patients with colorectal cancer. Promoter analysis revealed that the RP1 gene is under the control of a strong promoter that was 10 times more active in mammalian cells when compared to SV40 promoter. Members of the cyclic AMP response element binding protein family (CREB1 and CREB2) could be identi®ed as transcription factors binding speci®cally within the RP1 promoter sequence. Oncogene (2001) 20, 5920 ± 5929.

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Section: Discussionmentioning

confidence: 99%

Section: Characterization Of Protein/dna Interaction By Electromobilimentioning

confidence: 99%

Section: Polymerase Chain Reaction (Pcr) and Synthetic Oligo-nucleotidesmentioning

confidence: 99%

Section: Isolation Of the Genomic Rp1 Clonementioning

confidence: 99%

Section: Primer Extension Analysismentioning

confidence: 99%

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