Role of naofen in apoptosis of hepatocytes induced by lipopolysaccharide through mitochondrial signaling in rats

Fan, Jun; Feng, Guo; Huang, Lei; Tsunekawa, Koji; Honda, Takashi; Katano, Yoshiaki; Hirooka, Yoshiki; Goto, Hidemi; Kandatsu, Nobuhisa; Ando, Kazuo; Fujiwara, Yoshihiro; Koide, Tatsurou; Okada, Shoshiro; Ishikawa, Naohisa

doi:10.1111/j.1872-034x.2012.00972.x

Cited by 15 publications

(15 citation statements)

References 43 publications

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“…Previously, we reported that enhanced WDR35 expression may mediate apoptosis in several animal models [25,26]. As expected, expression of WDR35 was observed in neurons in the CA1 region of the rat hippocampus following DA administration (Figure 2G, 2I).…”

Section: Discussionsupporting

confidence: 83%

“…Quantitative RT-PCR was performed as described previously [26] with the ABI StepOne Plus real-time PCR system and a Taqman Gene Expression Assay (Applied Biosystems, Tokyo, Japan) according to the manufacturer’s instructions. WDR35 mRNA levels were quantified relative to GAPDH mRNA levels, the internal control.…”

Section: Methodsmentioning

confidence: 99%

“…WDR35 mRNA levels were quantified relative to GAPDH mRNA levels, the internal control. The results are presented according to the ΔΔC T method as ratios of the target to the internal control as described previously [26]. …”

Section: Methodsmentioning

confidence: 99%

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Enhanced expression of WD repeat-containing protein 35 (WDR35) stimulated by domoic acid in rat hippocampus: involvement of reactive oxygen species generation and p38 mitogen-activated protein kinase activation

et al. 2013

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BackgroundDomoic acid (DA) is an excitatory amino acid analogue of kainic acid (KA) that acts via activation of glutamate receptors to elicit a rapid and potent excitotoxic response, resulting in neuronal cell death. Recently, DA was shown to elicit reactive oxygen species (ROS) production and induce apoptosis accompanied by activation of p38 mitogen-activated protein kinase (MAPK) in vitro. We have reported that WDR35, a WD-repeat protein, may mediate apoptosis in several animal models. In the present study, we administered DA to rats intraperitoneally, then used liquid chromatography/ion trap tandem mass spectrometry (LC-MS/MS) to identify and quantify DA in the brains of the rats and performed histological examinations of the hippocampus. We further investigated the potential involvement of glutamate receptors, ROS, p38 MAPK, and WDR35 in DA-induced toxicity in vivo.ResultsOur results showed that intraperitoneally administered DA was present in the brain and induced neurodegenerative changes including apoptosis in the CA1 region of the hippocampus. DA also increased the expression of WDR35 mRNA and protein in a dose- and time-dependent manner in the hippocampus. In experiments using glutamate receptor antagonists, the AMPA/KA receptor antagonist NBQX significantly attenuated the DA-induced increase in WDR35 protein expression, but the NMDA receptor antagonist MK-801 did not. In addition, the radical scavenger edaravone significantly attenuated the DA-induced increase in WDR35 protein expression. Furthermore, NBQX and edaravone significantly attenuated the DA-induced increase in p38 MAPK phosphorylation.ConclusionIn summary, our results indicated that DA activated AMPA/KA receptors and induced ROS production and p38 MAPK phosphorylation, resulting in an increase in the expression of WDR35 in vivo.

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Section: Discussionsupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

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Enhanced expression of WD repeat-containing protein 35 (WDR35) stimulated by domoic acid in rat hippocampus: involvement of reactive oxygen species generation and p38 mitogen-activated protein kinase activation

et al. 2013

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“…The liver tissue often treated with pronase to eliminate parenchymal hepatocytes [23], however, pronase may destroys the lipopolysaccharide receptor CD14 on KCs [24]. Finally, obtained cells by percoll density gradient differential centrifugation [25], though the procedures are sophisticated and tedious (the time of centrifugation was more than 40 min in previous methods, while we needed fewer than 20 min).…”

Section: Discussionmentioning

confidence: 99%

A New Method to Isolate and Culture Rat Kupffer Cells

Zeng

Zhang

et al. 2013

PLoS ONE

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BackgroundPrevious methods for Kupffer cells (KCs) isolation require sophisticated skills and tedious procedures. Few studies have attempted to explore the self-renewal capacity of KCs in vitro. Therefore, the aim of this study was to establish a simple method for rat KCs isolation and further investigate the mitotic potential of KCs in vitro.MethodsKCs were obtained by performing one-step perfusion, enzymatic tissue treatment, differential centrifugation and selective adherence. The proliferation ability of cultured KCs was determined by MTT assay and Propidium Iodide FACS analysis. Phagocytic assay and ED-1, ED-2 immunofluorescence were used to identify cell phenotype. After stimulation with LPS, the expression of surface antigens (MHCII, CD40, CD80, and CD86) and the production of cytokines (NF-κB, TNF-α, IL-6 and IL-10) were measured for cell function identification.ResultsKCs were isolated with certain numbers and reasonable purities. The KCs were able to survive until at least passage 5 (P5), and at P3 showed equally strong phagocytic activity as primary KCs (P0). After stimulation with LPS, the change in the expression of surface antigens and the production of cytokines for P3 cells was similar to that for P0 cells.ConclusionsOur study provides a simple and efficient method for KCs isolation, and reveals that self-renewing KCs have the same phagocytic activity and functions as primary KCs.

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“…Therefore, the increase of activin A secretion is caused by enhanced production and secretion of activin A in AEC rather than an increased number of AEC. LPS induces apoptosis directly or indirectly in several types of cells [37–41]. Apoptosis of AEC occurs in fetal membranes from patients with chorioamnionitis [42].…”

Section: Discussionmentioning

confidence: 99%

Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells

Abe

Marukawa²,

et al. 2013

International Journal of Endocrinology

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Activin A is involved in inflammation. The present study was performed to clarify if lipopolysaccharide, a component of Gram-negative bacteria, stimulates activin A secretion from human amniotic epithelial cells and to determine if activin A plays a role in amnionitis. Fetal membranes were obtained during elective cesarean sections performed in full-term pregnancies of patients without systemic disease, signs of premature delivery, or fetal complications. Amniotic epithelial cells were isolated by trypsinization. The activin A concentrations in the culture media were measured by enzyme-linked immunosorbent assay, and cell proliferation was assessed by 5-bromo-2′-deoxyuridine incorporation. Amniotic epithelial cells secreted activin A in a cell density-dependent manner, and lipopolysaccharide (10 μg/mL) enhanced the secretion at each cell density. Lipopolysaccharide (10–50 μg/mL) also stimulated activin A secretion in a dose-dependent manner. Contrary to the effect of activin A secretion, lipopolysaccharide inhibited cell proliferation in amniotic epithelial cells. The present study suggests that lipopolysaccharide stimulation of activin A secretion may be a mechanism in the pathogenesis of amnionitis.

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Role of naofen in apoptosis of hepatocytes induced by lipopolysaccharide through mitochondrial signaling in rats

Abstract: These results indicate that LPS may induce the hepatic apoptosis in association with enhanced naofen expression, and that naofen may mediate the activation of caspase-3 through downregulating the Bcl-2 and Bcl-xL expression, and releasing cytochrome c from mitochondria to cytoplasm.

Cited by 15 publications

References 43 publications

Enhanced expression of WD repeat-containing protein 35 (WDR35) stimulated by domoic acid in rat hippocampus: involvement of reactive oxygen species generation and p38 mitogen-activated protein kinase activation

Enhanced expression of WD repeat-containing protein 35 (WDR35) stimulated by domoic acid in rat hippocampus: involvement of reactive oxygen species generation and p38 mitogen-activated protein kinase activation

A New Method to Isolate and Culture Rat Kupffer Cells

Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells

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