Role of naturally occuring autoantibodies in senescence of normal and ATP depleted goat erythrocytes

Abstract

SUMMARYThe possible role of autoantibodies in the senescence of goat erythrocytes has been investigated. For this purpose goat gammaglobulin was purified to homogenity and antigoat gammaglobulin was raised in rabbits. Using the peroxidase labelled antigoat gammaglobulin it was possible to detect the presence of auto anti band-3 antibodies in goat sera. The goat erythrocytes were aged in vitro by ATP-depletion, which resulted in appearence of a 52,000 mol. wt. polypeptide. The in vitro aged goat erythrocyte membrane bound substantial amounts of auto anti band-3 antibodies as visualized by immunoblots.