Role of Nitrate in the Induction of Nitrite Reductase Activity during Wheat Seed Germination

Gupta, Aditya K.; Disa, Suhas; Saxena, Inder M.; Sarin, Neera Bhalla; Guha‐Mukherjee, Sipra; Sopory, Sudhir K.

doi:10.1093/jxb/34.4.396

Cited by 8 publications

(6 citation statements)

References 18 publications

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“…W04'-, which inhibits the formation of active NR, did not inhibit induction of NiR by NOS-in wheat embryos (Gupta et al, 1983), tobacco cells (Kelker and Filner, 1971), and sunflower roots (de la Haba et al, 1990). This suggests that NO3-can induce NiR independently of NOz-.…”

mentioning

confidence: 90%

“…de la Haba et al (1990) reported that in sunflower roots 7N04'-completely inl~ibited the induction of NR without affecting the induction of NiR. W04'-also did not inhibit the induction of NiR by NO3-in barley leaves (Aslam et al, 1987), wheat embryos (Gupta et al, 1983), or tobacco cells (Kelker and Filner, 1971). Like the induction of NRA, the induction of NiR by both NO3-and NOz-was inhibited by CHI (Table VI), indicating the requirement for new protein synthasis.…”

Section: Lnduction Of Nirmentioning

confidence: 99%

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Comparative Induction of Nitrate and Nitrite Uptake and Reduction Systems by Ambient Nitrate and Nitrite in Intact Roots of Barley (Hordeum vulgare L.) Seedlings

1993

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California 9561 6 l h e induction by ambient NO3-and NOz-of the NOs-and NOzuptake and reduction systems in roots of 8-d-old intact barley (Hordeum vulgare 1.) seedlings was studied. Seedlings were induced with concentrations of NaN03 or NaNOz ranging from 0.25 to 1000 p~. Uptake was determined by measuring the depletion of either NO3-or NOz-from uptake solutions. Enzyme activities were assayed in vitro using cell-free extracts. Uptake and reduction systems for both NO3-and NOz-were induced by either ion. The K,,, values for NO3-and NOz-uptake induced by NOz-were similar to those for uptake induced by NO3-. lnduction of both the uptake and reduction systems was detected well before any NO3-or NOzwas found in the roots. At lower substrate concentrations of both NO3-and NOz-(5-10 PM), the durations of the lag periods preceding induction were similar. lnduction of uptake, as a function of concentration, proceeded linearly and similarly for both ions up to about 10 p~. lhen, while induction by NO3-continued to increase more slowly, induction by NOz-sharply decreased between 10 and 1000 p~, apparently due to NOz-toxicity. In contrast, induction of NO3-reductase (NR) and NOz-reductase (NiR) by NOz-did not decrease above 10 p~ but rather continued to increase up to a substrate concentration of 1000 p~. NO3-was a more effective inducer of NR than was NOz-; however, both ions equally induced NiR. Cycloheximide inhibited the induction of both uptake systems as well as NR and NiR activities whether induced by NO3-or NOz-. l h e results indicate that in situ NO3-and NOzinduce both uptake and reduction systems, and the accumulation of the substrates per se is not obligatory.

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mentioning

confidence: 90%

Section: Lnduction Of Nirmentioning

confidence: 99%

Comparative Induction of Nitrate and Nitrite Uptake and Reduction Systems by Ambient Nitrate and Nitrite in Intact Roots of Barley (Hordeum vulgare L.) Seedlings

1993

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“…Treatment of plant tissues with WO42-has often been used to separate the induction of NR and NiR by NO3-and NO2- (7,13). Tungstate is incorporated into NR in place of MoO42-making the enzyme inactive (19,25).…”

Section: Effect Of Tungstatementioning

confidence: 99%

“…largely inhibited by tungstate, NiR formation was not inhibited in wheat embryos (7) or in tobacco cells (13), further indicating that NO3-may induce NiR directly without being reduced to NO2-. Recently, Lahners et al (15) reported that both NO3-and N02-induced NiR-mRNA in leaves of maize seedlings.…”

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confidence: 91%

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Role of Nitrate and Nitrite in the Induction of Nitrite Reductase in Leaves of Barley Seedlings

Aslam¹,

Huffaker²

1989

Plant Physiol.

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The role of N03-and N02-in the induction of nitrite reductase (NiR) activity in detached leaves of 8-day-old barley (Hordeum vulgare L.) seedlings was investigated. Barley leaves contained 6 to 8 micromoles N02-/gram fresh weight x hour of endogenous NiR activity when grown in N-free solutions. Supply of both N02-and NO3-induced the enzyme activity above the endogenous levels (5 and 10 times, respectively at 10 millimolar NO2-and N03-over a 24 hour period). In N03-supplied leaves, NiR induction occurred at an ambient N03-concentration of as low as 0.05 millimolar; however, no NiR induction was found in leaves supplied with NO2-until the ambient NO2-concentration was 0.5 millimolar. Nitrate accumulated in N02-fed leaves. The amount of NO3-accumulating in N02-fed leaves induced similar levels of NiR as did equivalent amounts of NO3-accumulafing in N03--fed leaves. Induction of NiR in N02--fed leaves was not seen until N03-was detectable (30 nanomoles/gram fresh weight) in the leaves. The internal concentrations of N03-, irrespective of N source, were highly correlated with the levels of NiR induced.When the reduction of N03-to NO2-was inhibited by W042-, the induction of NiR was inhibited only partially. The results indicate that in barley leaves NiR is induced by N03-directly, i.e. without being reduced to N02-, and that absorbed NO2-induces the enzyme activity indirectly after being oxidized to N03-within the leaf.

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Soluble and Plasma Membrane-bound Enzymes Involved in Nitrate and Nitrite Metabolism

Meyer

Stöhr

2002

Advances in Photosynthesis and Respiration

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Role of Nitrate in the Induction of Nitrite Reductase Activity during Wheat Seed Germination

Cited by 8 publications

References 18 publications

Comparative Induction of Nitrate and Nitrite Uptake and Reduction Systems by Ambient Nitrate and Nitrite in Intact Roots of Barley (Hordeum vulgare L.) Seedlings

Comparative Induction of Nitrate and Nitrite Uptake and Reduction Systems by Ambient Nitrate and Nitrite in Intact Roots of Barley (Hordeum vulgare L.) Seedlings

Role of Nitrate and Nitrite in the Induction of Nitrite Reductase in Leaves of Barley Seedlings

Soluble and Plasma Membrane-bound Enzymes Involved in Nitrate and Nitrite Metabolism

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