Role of Nitric Oxide in the Colon of Patients With Slow-Transit Constipation

Tomita, Ryouichi; Fujisaki, Shigeru; Ikeda, Toshiki; Fukuzawa, Masahiro

doi:10.1007/s10350-004-6251-8

Cited by 91 publications

(83 citation statements)

References 25 publications

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“…Conversely, increased NO synthase-positive neurons and activities are associated with STC (Tomita et al, 2002;Wattchow et al, 2008). Our result is in agreement with the study by Mitolo-Chieppa et al (2001) performed in male STC circular muscle, which shows that EFS-induced relaxation of SP-precontracted muscle strips is reduced at 0.5 Hz (ATP mediated) and enhanced at 4 Hz (NO) medicated compared with control, suggesting an abnormality in NO and ATP release.…”

Section: Discussionsupporting

confidence: 92%

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK₁ Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation

Liu

Shang²,

Morgan³

et al. 2009

J Pharmacol Exp Ther

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Tachykinins are important neurotransmitters regulating intestinal motility. Slow transit constipation (STC) represents an extreme colonic dysmotility with unknown etiology that predominantly affects women. We examined whether the tachykinin system is involved in the pathogenesis of STC. Isolated sigmoid colon circular muscle from female STC and control patients was studied using functional and quantitative reverse transcriptase-polymerase chain reaction methods. A possible alteration of neurotransmission was investigated by electrical field stimulation (EFS) and ganglionic stimulation by dimethylphenylpiperazinium (DMPP). Substance P (SP)-mediated contractions in circular muscle strips were significantly diminished in STC compared with age-matched control (P Ͻ 0.001). In contrast, contractile responses to neurokinin A, the selective tachykinin NK 2 receptor agonist, [Lys to SP in STC were fully restored by indomethacin, partially reversed by tetrodotoxin (TTX), but unaffected by atropine or hexamethonium. The restoration by indomethacin was blocked by the NK 1 receptor antagonist CP99994 [(2S,3S)-3-(2-methoxybenzylamino)-2-phenylpiperidine] and TTX. In STC colonic muscle, there was a significant increase of NK 1 receptor mRNA expression, but no difference in NK 2 mRNA level. DMPP generated biphasic responses, relaxation at lower and contraction at higher concentrations. Although the responses to DMPP were similar in STC and control, an altered contractile pattern in response to EFS was observed in STC circular muscle. In conclusion, we postulate that the diminished contractile response to SP in STC is due to an increased release of inhibitory prostaglandins through activation of up-regulated NK 1 receptors. Our results also indicate some malfunction of the enteric nervous system in STC.

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Section: Discussionsupporting

confidence: 92%

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK₁ Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation

Liu

Shang²,

Morgan³

et al. 2009

J Pharmacol Exp Ther

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“…52 Often, the pressure required is found to be higher than in subjects with normal colonic transit. 53 Various physiologic and histobio-chemical findings have been postulated to explain the phenomenon of slow colonic transit: reduced cholinergic 54,55 and enhanced adrenergic responses 55 ; mitigated gastrocolic reflex 56 ; dysynergy of rectosigmoid colonic activities 57,58 ; enteric neurodegeneration 59 of both the myenteric plexus ganglia 60,61 and the interstitial cells of Cajal 61,62 ; and abnormalities of enteric neurotransmitters such as substance P, [63][64][65] vasoactive intestinal peptides, 63,64 and nitric oxide.…”

Section: Intrinsic Factorsmentioning

confidence: 99%

Chronic Constipation: An Evidence-Based Review

Leung¹,

Riutta²,

Kotecha³

et al. 2011

The Journal of the American Board of Family Medicine

273

168

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Background: Chronic constipation is a common condition seen in family practice among the elderly and women. There is no consensus regarding its exact definition, and it may be interpreted differently by physicians and patients. Physicians prescribe various treatments, and patients often adopt different over-the-counter remedies. Chronic constipation is either caused by slow colonic transit or pelvic floor dysfunction, and treatment differs accordingly.Methods: To update our knowledge of chronic constipation and its etiology and best-evidence treatment, information was synthesized from articles published in PubMed, EMBASE, and Cochrane Database of Systematic Reviews. Levels of evidence and recommendations were made according to the Strength of Recommendation taxonomy.Results

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“…NOS inhibits the release of AchE, and NOS and VIP exhibit a close morphological relationship that is directly associated with constipation. Excessive NOS may lead to constipation and a decrease of other factors that are beneficial to the intestine (28).…”

Section: Discussionmentioning

confidence: 99%

Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation

Li¹,

Zou²,

Kuang³

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. The present study aimed to determine the preventative effects of fermented Chimonobambusa quadrangularis shoot (FCQS) on activated carbon constipation in Kun Ming mice. FCQS has a more loose fiber tissue structure than unfermented fresh C. quadrangularis shoot (CQS), which is preferable for relieving constipation. In mice fed with FCQS for 9 days the time from consumption to their first black stool defecation (117 min) was shorter than the control group (192 min) and the CQS group (148 min); however, it was longer than the normal (85 min) and bisacodyl treatment (99 min) groups. The gastrointestinal transit of the FCQS group (73.8%) was increased, as compared with the control (37.9%) and CQS (61.7%) groups; however, it was decreased as compared with the normal (100%) and bisacodyl (88.3%) groups. By observing the hemotoxylin and eosin-stained section of mice intestine, it was demonstrated that FCQS reduced injury to the intestinal tract resulting from constipation and alleviated the damage caused to the intestinal villi over the effects observed in the CQS group. Furthermore, FCQS was also able to increase the serum levels of motilin, endothelin-1, vasoactive intestinal peptide and acetylcholinesterase compared with the control group. c-Kit, stem cell factor (SCF), glial cell-derived neurotrophic factor (GDNF) mRNA and protein expression levels in the small intestinal cells of FCQS-fed mice were increased, as compared with CQS-fed mice. Transient receptor potential cation channel subfamily V member 1 (TRPV1) and nitric oxide synthase (NOS) expression levels of small intestinal cells of FCQS-fed mice were reduced, as compared with CQS-fed mice. These findings demonstrated that FCQS may induce improved preventative effects on constipation, compared with CQS.

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Role of Nitric Oxide in the Colon of Patients With Slow-Transit Constipation

Abstract: These findings suggest that an increase of nitric oxide mediates nonadrenergic, noncholinergic inhibitory nerves and plays an important role in the dysmotility observed in the colons of patients with slow-transit constipation.

Cited by 91 publications

References 25 publications

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK₁ Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK₁ Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation

Chronic Constipation: An Evidence-Based Review

Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation

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Role of Nitric Oxide in the Colon of Patients With Slow-Transit Constipation

Abstract: These findings suggest that an increase of nitric oxide mediates nonadrenergic, noncholinergic inhibitory nerves and plays an important role in the dysmotility observed in the colons of patients with slow-transit constipation.

Cited by 91 publications

References 25 publications

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK1 Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK1 Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation

Chronic Constipation: An Evidence-Based Review

Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation

Contact Info

Product

Resources

About

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK₁ Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation

Cyclooxygenase-Dependent Alterations in Substance P-Mediated Contractility and Tachykinin NK₁ Receptor Expression in the Colonic Circular Muscle of Patients with Slow Transit Constipation