Role of nitric oxide in immune responses against viruses: beyond microbicidal activity

Uehara, Elaine Uchima; Shida, Beatriz de Stefano; Brito, Cyro Alves de

doi:10.1007/s00011-015-0857-2

Cited by 85 publications

(77 citation statements)

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“…It is proved that infection with CVS strain of RABV in mice resulted in 30-fold increase in NO levels [16,23], iNOS [16,40,41] and iNOS enzyme activity [16,17,33]. Also, it was well established that high concentration of NO showed immunosuppressive action by its anti-proliferative effects on T cells [24,26,30,42], blocking of IL-2R signaling [27,30] and induced T cell apoptosis by caspase or Fas or p53-dependent mechanisms [25,26,29,43]. Recently, we reported that CVS strain of RABV up regulates caspase 1 and FasL mRNA expressions and more TUNEL positive apoptotic bodies in brain, which was positively correlated with intensity of clinical signs and mortality [16].…”

Section: Discussionmentioning

confidence: 98%

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Role of nitric oxide in the regulation of immune responses during rabies virus infection in mice

et al. 2016

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Rabies virus (RABV) stimulates nitric oxide (NO) production, which either triggers T cell differentiation or suppresses T cell function depending on its concentration. Herein, we assessed the potential role of NO in regulation of immune responses during RABV infection in mice model. The experimental animals were divided into four groups and 100LD 50 of challenge virus standard (CVS) strain of RABV was inoculated intracerebrally on day 0 and subsequently aminoguanidine (AG; inducible nitric oxide synthase inhibitor) was injected intraperitoneally twice a day, up to 6 days. The samples were collected at 2, 4, 6, 8, 9, 10 and 12 days post infection (DPI). The immune cells including CD4? , CD8 ? T lymphocytes and natural killer (NK) cells were estimated from peripheral blood mononuclear cells (PBMCs) and splenocytes. Serum total NO concentration, histopathology, immunohistochemistry, direct fluorescent antibody technique and TUNEL assay was performed. Infection with CVS resulted in significant early increase in CD4? , CD8?and NK cells in blood and spleen until 2 DPI. From 4 DPI onwards significant reduction was noticed in these parameters which coincided with increased NO on 4 DPI, rising to maximum on 8 DPI, until their death on 10 DPI. Conversely, the CVS-AG treated group showed lower levels of NO and increased number of CD4 ? , CD8 ? and NK cells. Increased number of cells in blood and spleen coincided with increased survival time, delayed development of clinical signs, reduced viral load and less apoptotic cells. NO played important role in regulation of immune responses during RABV infection. The findings of present study confirmed the role of NO and/or iNOS using iNOS inhibitor (aminoguanidine) in immune response during RABV infection, which would further help in understanding the virus immunopathogenesis with adoption of newer antiviral strategies to counter the progression of disease.

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Section: Discussionmentioning

confidence: 98%

“…It also participates in immune response regulation, particularly in the adaptive response by controlling T lymphocyte proliferation [24][25][26]. Further, NO exerts either proliferative or anti-proliferative response on T cells depending on its concentration.…”

Section: Introductionmentioning

confidence: 99%

Role of nitric oxide in the regulation of immune responses during rabies virus infection in mice

et al. 2016

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“…Stimulation of macrophages with LPS increases expression of the iNOS gene and promotes excessive NO release via decomposition of L-arginine. iNOS is also activated in response to inflammatory stimuli such as cytokines, IL, and bacterial endotoxin (33,34). In addition to its role in NO release, IL-1β also serves as one of the major pro-inflammatory cytokines released following LPS stimulation of macrophages, and its excessive production has been linked to development of chronic inflammatory diseases (35,36).…”

Section: Discussionmentioning

confidence: 99%

An ethanol extract of <i>Aster yomena</i> (Kitam.) Honda inhibits lipopolysaccharide-induced inflammatory responses in murine RAW 264.7 macrophages

Kang

Jeong

Park³

et al. 2017

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“…O aumento de óxido nítrico (NO) leva ao aumento da toxicidade endógena e, como consequência, uma diminuição da taxa autóctone de microorganismos da boca (Bajaj et al, 2015).Por causa de sua função microbicida, NO tem sido frequentemente considerado um mediador protetor em infecções virais (Uehara et al, 2015). Em geral, a modificação de proteínas pelo NO afeta a replicação viral.…”

Section: Discussionunclassified

Detecção dos poliomavirus humano JC e BK em pacientes no pré transplante de fígado

Figueiredo¹

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AGRADECIMENTOSAgradeço primeiramente à Deus por tornar possível este sonho.Aos meus pais, Pascoal e Sonia, meus maiores incentivadores. Foram minha força e apoio todos os dias, sem eles nada seria possível. Obrigada pela paciência, carinho, amor e garra que vocês me ensinaram a ter.A minha irmã Alessandra, pelo apoio que me ajudou a concluir este trabalho.Ao meu sobrinho Rafael pela alegria que me traz sempre.À minha orientadora Karem Ortega pelos conselhos, paciência, ensinamentos, incentivo e confiança, para a realização deste trabalho.É uma honra tê-la como orientadora! Aos professores Paulo Braz e Maria Cristina Fink, pelos ensinamentos e auxílio.Obrigada pela parceria e por tornarem este trabalho possível.A Jeanne, sem ela as coletas não teriam sido possíveis, obrigada por me ensinar e ajudar sempre.Ao Frederico Buhatem, pela amizade, confiança e ensinamentos, sem ele este mestrado também não seria possível.Aos professores do Departamento de Patologia Bucal da FOUSP, Marina, Suzana, Marília, Décio e Fábio Daumas.Aos meus queridos amigos da pós-graduação, em especial Karin, Talita, Dani, Cíntia, Rubens, Dmytri, Natalia e Nathalia. Vocês foram essenciais no dia a dia.Obrigada pelo carinho e apoio sempre! Às minhas amigas e colegas de trabalho do CAPE-FOUSP, Gil, Sandra, Cris, Andressa, Fatima e Leninha. Obrigada pelo companheirismo, pela amizade, pelas conversas e risadas. Vocês fazem parte deste trabalho! Aos funcionários da secretaria do departamento de Patologia Bucal, Vinícius e Fátima por toda a orientação e auxílio em todos os momentos desse meu curso de mestrado.As minhas amigas Aline e Carol, que me apoiaram sempre. Furthermore, these patients may also have renal impairment (hepatorenal syndrome) and central nervous system (hepatic encephalopathy). With complications like these and how these patients present with impaired current cellular immune response, it may be interesting to investigate the presence of polyomavirus in these patients. The objective of this study was to identify and quantify BKV and JCV polyomavirus in saliva, oral lavage, blood and urine of patients before liver transplantation. For the study group were selected, 21 cirrhotic patients, liver transplant waiting list, of both sexes, aged over 18 and for the control group were selected 20 normorreativos patients of both sexes, older than 18 years. In these patients fluid collections were performed, urine, saliva, oral lavage and blood. After collecting and freezing the samples were carried out research of detection and quantification of human polyomavirus BK and JC subtypes using the PCR method in real time. Fifteen patients (71.42%) of GE were positive for BKV in at least one sample, but none of the patients in this group had hepatorenal syndrome. Moreover, 66.66% of patients had hepatic encephalopathy, but while 10 patients were positive for JCV, none of them has been able to quantify the viral load or the presence of virus associated with clinical disease. As regards the samples, the GE 21 (25%) were positive for BKV and 10 (11.90...

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Role of nitric oxide in immune responses against viruses: beyond microbicidal activity

Cited by 85 publications

References 62 publications

Role of nitric oxide in the regulation of immune responses during rabies virus infection in mice

Role of nitric oxide in the regulation of immune responses during rabies virus infection in mice

An ethanol extract of <i>Aster yomena</i> (Kitam.) Honda inhibits lipopolysaccharide-induced inflammatory responses in murine RAW 264.7 macrophages

Detecção dos poliomavirus humano JC e BK em pacientes no pré transplante de fígado

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