Role of Noninvasive Tests (<sup>13</sup>C‐Urea Breath Test and Stool Antigen Test) as Additional Tools in Diagnosis of <i>Helicobacter Pylori</i> Infection in Patients with Atrophic Body Gastritis

Lahner, Edith; Vaira, Dino; Figura, Natale; Pilozzi, Emanuela; Pasquali, Alessandra; Severi, Carola; Perna, Federico; Fave, Gianfranco Delle; Annibale, Bruno

doi:10.1111/j.1083-4389.2004.00262.x

Cited by 41 publications

(47 citation statements)

References 30 publications

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“…The stool antigen test may be performed by conventional immunoassay (EIA) using polyclonal antibodies 4,6,7,10,13,17 , showing sensitivity of 88.9% to 98.3% and specificity of 77.8% to 98.4%, and by rapid lateral flow immunoassay using monoclonal antibodies 10,12,14,15,33 , presenting 52.5-94.6% sensitivity, 55.5-98.4% specificity, 94.6% positive predictive value and 98.4% of negative predictive value 2,10,12,33 . The lateral flow immunoassay is useful for small laboratories that do not have equipment for performing the EIA and that work with few samples.…”

Section: Discussionmentioning

confidence: 99%

“…Breath samples were analyzed by infrared spectroscopy (IRIS DOC, Wagner AnalysenTechnik, Bremen, Germany). A DOB (Delta over baseline-value) ≥ 4.0‰ was considered positive for H. pylori infection, as previously described 17 .…”

Section: C-urea Breath (Ubt)mentioning

confidence: 99%

“…Recently, a noninvasive diagnostic test based on the detection of H. pylori stool antigen has been developed. Some H. pylori stool antigen tests that use polyclonal antibodies to H. pylori have shown heterogeneous results 2,12,17 . More recently, novel stool antigen tests based on monoclonal antibodies which could increase the accuracy of this test, have been developed 2,8,12,14,15,33 .…”

Section: Introductionmentioning

confidence: 99%

“…In Brazil, previous validation of a monoclonal stool antigen test for diagnosis of H. pylori was reported in children 27 ; however, the stool antigen test based on lateral flow chromatography has not yet been tested in adults. The major advantage of these tests is the cost, when compared to 13 C-UBT and the possibility of being performed in any laboratory 10,14,15,17 .…”

Section: Introductionmentioning

confidence: 99%

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Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Silva

Villares

Monteiro

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe aim of this study was to validate the rapid lateral flow Helicobacter pylori stool antigen test (One step H. pylori antigen test, ACON laboratories, San Diego, USA; Prime diagnostics, São Paulo), using 13 C-Urea Breath Test as the gold standard for H. pylori infection diagnosis. A total of 98 consecutive patients, asymptomatic or dyspeptic, entered the study. Sixty-nine were women, with a mean age of 45.76 ± 14.59 years (14 to 79 years). In the H. pylori-positive group, the rapid stool antigen test detected H. pylori antigen in 44 of the 50 positive patients (sensitivity 88%; 95% CI: 75.7-95.5%), and six false-negative; and in the H. pylori-negative group 42 presented negative results (specificity 87.5%; 95% CI: 74.7-95.3%), and six false-positive, showing a substantial agreement (Kappa Index = 0.75; p < 0.0001; 95% CI: 0.6-0.9). Forty four of fifty patients that had positive stool antigen were H. pylori-positive, the PPV of the stool antigen test was 88% (95% CI: 75.7-95.5%), and 42 patients with negative stool antigen test were H. pylori-negative, the NPV of the stool antigen test was 87.5% (95% CI: 74.7-95.3%). We conclude that the lateral flow stool antigen test can be used as an alternative to breath test for H. pylori infection diagnosis especially in developing countries.

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Section: Discussionmentioning

confidence: 99%

Section: C-urea Breath (Ubt)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Silva

Villares

Monteiro

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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“…Breath samples were analyzed by infrared spectroscopy (IRIS, Wagner Analysen-Technik, Bremen, Germany). A DOB > 4.5% was considered positive for Hp infection, as previously described (8).…”

Section: Diagnostic Procedures Urea Breath Test (Ubt)mentioning

confidence: 99%

Chronic Idiophatic Urticaria and Helicobacter Pylori: A Specific Pattern of Gastritis and Urticaria Remission after Helicobacter Pylori Eradication

Persechino

Annibale²,

Caperchi

et al. 2012

Int J Immunopathol Pharmacol

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Chronic urticaria (CU) is defined as the occurrence of spontaneous wheals for a duration of more than 6 weeks and is the most frequent skin disease, with prevalence ranging between 15 and 25%, and is a seriously disabling condition, with social isolation and mood changes causing a significant degree of dysfunction and quality of life impairment to many patients. The main clinical features of CU are the repeated occurrence of transient eruptions of pruritic wheals or patchy erythema on the skin that last less than 24 hours and disappear without sequelae. CU is often defined as Chronic idiopathic urticaria (CIU) because the causes of CU remain unknown in the great majority (70-95%) of patients. Drugs, food, viruses, alimentary conservative substances or inhalant substances often seem to be involved in determining CIU skin flare. Despite a general agreement that bacteria infections and parasitic infestations can be involved in the pathogenesis of CIU, proven evldence of these relationships is lacking. The aim of the present study is to evaluate the prevalence of Helicobacter pylori (Hp) infection, and the extension and severity of gastritis in a group of CIU patients compared to controls and to evaluate the effectiveness of eradication of Hp on the CIU symptomatology, and the role of Hp infection in pathogenesis of CIU.Helicobacter pylori (Hp), a microaerophilic gram negative bacterium, was first isolated from the human stomach by Marshall and Warren in 1983. It is an ubiquitous infection in the adult population. The prevalence among middle-aged adults is over 80% in many developing countries, as compared with 20% to 50% in industrialized countries. Mainly, the infection is acquired in infancy and is generally transmitted within families in early childhood. Hp has been demonstrated as the cause of gastritis and peptic ulcer and as being associated to gastric cancer and mucosa-associated-lymphoma i.e. MALT lymphoma (1-3). There is evidence that Hp infection is associated with extra-digestive pathologies such as autoimmune, vascular and skin diseases. The pathogenic mechanisms involved are related to the persistent inflammatory response to Hp infection with release of immune associated substances, increase in gastric permeability and/or release of mediators acting on cutaneous sensitivity. In recent studies a possible association between Hp infection and CIU has been suggested. In fact, in some studies,

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Non-invasive diagnostic tests forHelicobacter pyloriinfection

Best

Takwoingi

Siddique

et al. 2018

Cochrane Database of Systematic Reviews

158

130

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In people without a history of gastrectomy and those who have not recently had antibiotics or proton ,pump inhibitors, urea breath tests had high diagnostic accuracy while serology and stool antigen tests were less accurate for diagnosis of Helicobacter pylori infection.This is based on an indirect test comparison (with potential for bias due to confounding), as evidence from direct comparisons was limited or unavailable. The thresholds used for these tests were highly variable and we were unable to identify specific thresholds that might be useful in clinical practice.We need further comparative studies of high methodological quality to obtain more reliable evidence of relative accuracy between the tests. Such studies should be conducted prospectively in a representative spectrum of participants and clearly reported to ensure low risk of bias. Most importantly, studies should prespecify and clearly report thresholds used, and should avoid inappropriate exclusions.

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Role of Noninvasive Tests (¹³C‐Urea Breath Test and Stool Antigen Test) as Additional Tools in Diagnosis of Helicobacter Pylori Infection in Patients with Atrophic Body Gastritis

Cited by 41 publications

References 30 publications

Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Chronic Idiophatic Urticaria and Helicobacter Pylori: A Specific Pattern of Gastritis and Urticaria Remission after Helicobacter Pylori Eradication

Non-invasive diagnostic tests forHelicobacter pyloriinfection

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Role of Noninvasive Tests (13C‐Urea Breath Test and Stool Antigen Test) as Additional Tools in Diagnosis of Helicobacter Pylori Infection in Patients with Atrophic Body Gastritis

Cited by 41 publications

References 30 publications

Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Chronic Idiophatic Urticaria and Helicobacter Pylori: A Specific Pattern of Gastritis and Urticaria Remission after Helicobacter Pylori Eradication

Non-invasive diagnostic tests forHelicobacter pyloriinfection

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Role of Noninvasive Tests (¹³C‐Urea Breath Test and Stool Antigen Test) as Additional Tools in Diagnosis of Helicobacter Pylori Infection in Patients with Atrophic Body Gastritis