Role of Notch Signaling in Leg Development in Drosophila melanogaster

Córdoba, Sergio; Estella, Carlos

doi:10.1007/978-3-030-34436-8_7

Cited by 14 publications

(22 citation statements)

References 147 publications

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“…We did not observe any morphological defects in the eye and wing of homozygous and hemizygous amx Δ flies at all temperatures tested (between 18–29°C). We also looked at other tissues that are often affected when Notch signaling is defective including the nota and legs [ 34 , 35 ] but we did not observe any morphological phenotypes in these tissues either. In summary, amx Δ is the first clean loss-of-function (LoF) allele of amx generated by CRISPR/Cas9, and phenotypically resembles the classic amx 1 allele rather than the amx m allele.…”

Section: Resultsmentioning

confidence: 99%

TM2D genes regulate Notch signaling and neuronal function in Drosophila

et al. 2021

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TM2 domain containing (TM2D) proteins are conserved in metazoans and encoded by three separate genes in each model organism species that has been sequenced. Rare variants in TM2D3 are associated with Alzheimer’s disease (AD) and its fly ortholog almondex is required for embryonic Notch signaling. However, the functions of this gene family remain elusive. We knocked-out all three TM2D genes (almondex, CG11103/amaretto, CG10795/biscotti) in Drosophila and found that they share the same maternal-effect neurogenic defect. Triple null animals are not phenotypically worse than single nulls, suggesting these genes function together. Overexpression of the most conserved region of the TM2D proteins acts as a potent inhibitor of Notch signaling at the γ-secretase cleavage step. Lastly, Almondex is detected in the brain and its loss causes shortened lifespan accompanied by progressive motor and electrophysiological defects. The functional links between all three TM2D genes are likely to be evolutionarily conserved, suggesting that this entire gene family may be involved in AD.

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Section: Resultsmentioning

confidence: 99%

TM2D genes regulate Notch signaling and neuronal function in Drosophila

et al. 2021

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“…Besides regulating cell-fate decisions at an individual cell level, a cell-to-cell signaling mechanism of Notch coordinates the spatiotemporal patterning in a tissue [ 20 ]. In Drosophila melanogaster , Notch functions as it is required to specify the fate of the cells that will eventually segment the leg and develop the leg joint [ 21 , 22 ]. The mechanisms of BMP gradient formation have been studied in various animals.…”

Section: Data Descriptionmentioning

confidence: 99%

Chromosome-level genome assembly of Plazaster borealis sheds light on the morphogenesis of multiarmed starfish and its regenerative capacity

Lee¹,

Kim

Jung

et al. 2022

GigaScience

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Background Plazaster borealis has a unique morphology, displaying multiple arms with a clear distinction between disk and arms, rather than displaying pentaradial symmetry, a remarkable characteristic of echinoderms. Herein we report the first chromosome-level reference genome of P. borealis and an essential tool to further investigate the basis of the divergent morphology. Findings In total, 57.76 Gb of a long read and 70.83 Gb of short-read data were generated to assemble a de novo 561-Mb reference genome of P. borealis, and Hi-C sequencing data (57.47 Gb) were used for scaffolding into 22 chromosomal scaffolds comprising 92.38% of the genome. The genome completeness estimated by BUSCO was 98.0% using the metazoan set, indicating a high-quality assembly. Through the comparative genome analysis, we identified evolutionary accelerated genes known to be involved in morphogenesis and regeneration, suggesting their potential role in shaping body pattern and capacity of regeneration. Conclusion This first chromosome-level genome assembly of P. borealis provides fundamental insights into echinoderm biology, as well as the genomic mechanism underlying its unique morphology and regeneration.

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“…We did not observe any morphological defects in the eye and wing of homozygous and hemizygous amx D flies at all temperatures tested (between 18-29°C). We also looked at other tissues that are often affected when Notch signaling is defective including the notum and legs (Córdoba and Estella, 2020;Schweisguth, 2015) but we did not observe any morphological phenotypes in these tissues either. In summary, amx D is the first clean loss-of-function (LoF) allele of amx generated by CRISPR/Cas9, and phenotypically resembles the classic amx allele rather than the amx m allele.…”

Section: A Clean Null Allele Of Amx Fully Recapitulates Previously Reported Maternal-effect Phenotypesmentioning

confidence: 89%

Alzheimer’s disease-associatedTM2Dgenes regulate Notch signaling and neuronal function inDrosophila

Salazar

Yang

et al. 2021

Preprint

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TM2 domain containing (TM2D) proteins are conserved in metazoans and encoded by three separate genes in each species. Rare variants in TM2D3 are associated with Alzheimer's disease (AD) and its fly ortholog almondex is required for embryonic Notch signaling. However, the functions of this gene family remain elusive. We knocked-out all three TM2D genes (almondex, CG11103/amaretto, CG10795/biscotti) in Drosophila and found that they share the same maternal-effect neurogenic defect. Triple null animals are not phenotypically worse than single nulls, suggesting these genes function together. Overexpression of the most conserved region of the TM2D proteins acts as a potent inhibitor of Notch signaling at the γ-secretase cleavage step. Lastly, Almondex is detected in the brain and its loss causes shortened lifespan accompanied by progressive electrophysiological defects. The functional links between all three TM2D genes are likely to be evolutionarily conserved, suggesting that this entire gene family may be involved in AD.

show abstract

Role of Notch Signaling in Leg Development in Drosophila melanogaster

Cited by 14 publications

References 147 publications

TM2D genes regulate Notch signaling and neuronal function in Drosophila

TM2D genes regulate Notch signaling and neuronal function in Drosophila

Chromosome-level genome assembly of Plazaster borealis sheds light on the morphogenesis of multiarmed starfish and its regenerative capacity

Alzheimer’s disease-associatedTM2Dgenes regulate Notch signaling and neuronal function inDrosophila

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