Role of Npm1 in proliferation, apoptosis and differentiation of neural stem cells

Yang, Qing; Gao, Yue; Lu-jun, Bing; Li, Shaoling

doi:10.1016/j.jns.2007.09.029

Cited by 30 publications

(33 citation statements)

References 36 publications

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“…Our results are consistent with findings described by Qing et al, 44 who observed suppression of Npm1 in neural stem cells undergoing differentiation. Currently, the role of ribosomal assembly, and Nucleophosmin 1 in neural cell differentiation is not fully understood.…”

Section: Research Articlessupporting

confidence: 94%

Nuclear Proteome Dynamics in Differentiating Embryonic Carcinoma (NTERA-2) Cells

et al. 2010

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The use of stem cells for generating cell types suitable for therapy is dependent on understanding the mechanisms, and identifying biomarkers, that control cell fate into different lineages. In this study, we aimed to characterize the nuclear protein dynamics of NTERA-2 cells undergoing retinoic acid-induced differentiation. We focused specifically on the first six days of differentiation, to provide insight into the earliest differentiation events, and employed techniques to specifically monitor the nuclear proteome. Well-characterized gene expression markers were used to precisely stage cell differentiation across the experimental time course. A combination of the novel iTRAQ and ExacTag labeling technologies, together with LC-ESI tandem mass spectrometry, were then used to accurately measure nuclear protein expression changes occurring within these differentiation-staged cells. We report proteins that showed significantly altered expression over the first 6 days of differentiation. Extensive bioinformatic analysis was undertaken, resulting in the construction of a novel interactome network, which revealed the temporal dynamics of the nuclear protein network in the context of neuronal differentiation.

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Section: Research Articlessupporting

confidence: 94%

Nuclear Proteome Dynamics in Differentiating Embryonic Carcinoma (NTERA-2) Cells

et al. 2010

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“…The involvement of NPM in the increased proliferation and disruption of differentiation in different types of cells has been previously reported (9,33). E6/E7-expressing HFKs can override the normal process of differentiation, and in this present report we provide the first evidence that increased levels of NPM in HFKs are important for inhibition of differentiation and proliferation.…”

Section: Discussionsupporting

confidence: 83%

“…In this study, we investigated the possible role of the nucleolar phosphoprotein nucleophosmin (NPM) in the differentiation process of HFKs. Frequently overexpressed in tumors and highly proliferating cells, NPM has previously been shown to reduce the susceptibility of cells to the onset of differentiation and apoptosis (13,33). NPM has also been reported to play a crucial role in sustaining ribosome biogenesis in cancer cells (12) and is now regarded as important in the development of various cancers (9,13,22), with the first reported NPM inhibitor developed as an anticancer agent in 2008 (32).…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus Type 16 E6/E7 Upregulation of Nucleophosmin Is Important for Proliferation and Inhibition of Differentiation

McCloskey

Menges

Friedman

et al. 2010

J Virol

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The E6 and E7 oncoproteins of high-risk human papillomaviruses (HPVs) are together sufficient to cause cellular transformation. Nucleophosmin (NPM) was identified as a protein with increased levels in twodimensional (2-D) gel analysis of human foreskin keratinocytes (HFKs) expressing E7 following methylcellulose-induced differentiation. Analysis of NPM expression in E7-expressing cells and E6-and E7-expressing cells in culture and in organotypic rafts confirmed the increased levels observed in 2-D gel analysis. The elevated expression of NPM was determined to be posttranscriptional and was attributed to increased v-akt murine thymoma viral oncogene (AKT) activity in the E6-and E7-expressing cells. Depletion of NPM caused a reduction in the replicative capacity of E7-and E6/E7-expressing HFKs and an increase in markers of differentiation. Also, the p53 and pRb tumor suppressor levels are increased with the knockdown of NPM in E6/E7-expressing cells, and, interestingly, p14 ARF is relocalized from the nucleolus to the nucleoplasm and cytoplasm in these cells. The results show for the first time that NPM is required for the proliferation and inhibition of differentiation observed in HPV E6-and E7-expressing primary cells.

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“…2H 00 and I 00 ). As nucleophosmin is overexpressed in rapidly dividing cells and its downregulation suppresses proliferation (Wang et al 2006, Okuwaki 2008, Qing et al 2008, the elevated expression in TE cells is likely in connection with preparation for embryo implantation. Correspondingly, Colombo et al (2005) and Grisendi et al (2005) showed that the placental structures in NPM1 K/K mice embryos are smaller, even though normally developed.…”

Section: Discussionmentioning

confidence: 99%

Bovine preimplantation embryos with silenced nucleophosmin mRNA are able to develop until the blastocyst stage

Toralova¹,

Benesova²,

Kepková³

et al. 2012

Reproduction

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This study was conducted to investigate the effect of silencing nucleophosmin in the development of in vitro-produced bovine embryos. Nucleophosmin is an abundant multifunctional nucleolar phosphoprotein that participates, for example, in ribosome biogenesis or centrosome duplication control. We showed that although the transcription of embryonic nucleophosmin started already at late eightcell stage, maternal protein was stored throughout the whole preimplantation development and was sufficient for the progression to the blastocyst stage. At the beginning of embryogenesis, translation occurs on maternally derived ribosomes, the functionally active nucleoli emerge during the fourth cell cycle in bovines. We found that nucleophosmin localisation reflected the nucleolar formation during bovine preimplantation development. The protein was detectable from the beginning of embryonic development. Before embryonic genome activation, it was dispersed throughout the nucleoplasm. The typical nucleolar localisation emerged with the formation of active nucleoli. At the blastocyst stage, nucleophosmin tended to localise especially to the trophectoderm. To see for how long is maternal nucleophosmin preserved, we silenced the nucleophosmin mRNA using RNA interference approach. Although a large portion of nucleophosmin was degraded in embryos with silenced nucleophosmin mRNA, an amount sufficient for normal development was preserved and we detected only a temporal delay in nucleophosmin relocalisation to nucleoli. Moreover, we observed no defects in nuclear shape or cytoskeleton previously found in somatic cells and only a non-significant decrease in embryonic developmental competence. Thus, our results show that the preserved amount of maternal nucleophosmin is sufficient for preimplantation development of bovine embryo.

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Role of Npm1 in proliferation, apoptosis and differentiation of neural stem cells

Cited by 30 publications

References 36 publications

Nuclear Proteome Dynamics in Differentiating Embryonic Carcinoma (NTERA-2) Cells

Nuclear Proteome Dynamics in Differentiating Embryonic Carcinoma (NTERA-2) Cells

Human Papillomavirus Type 16 E6/E7 Upregulation of Nucleophosmin Is Important for Proliferation and Inhibition of Differentiation

Bovine preimplantation embryos with silenced nucleophosmin mRNA are able to develop until the blastocyst stage

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