Role of Nuclear Morphometry as Objective Parameter to Evaluate Cytology Smears of Epithelial Breast Lesions

Chowdhury, Ananda S.; Talukdar, Manas; Adhikari, Anjan

doi:10.7860/jcdr/2017/30799.10957

Cited by 2 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many authors have studied different nuclear morphometric parameters including size and shape parameters like our study. [ 5 6 7 8 9 10 14 15 16 17 ] In the present study, six nuclear parameters including size parameters (nuclear area, perimeter, and diameter), density (integrated and raw) parameters measuring nuclear chromasia, and axis ratio (shape parameter) were assessed morphometrically. All these parameters except the axis ratio showed statistically significant differences with P < 0.05.…”

Section: Discussionmentioning

confidence: 99%

“…In order to overcome this subjectivity, studies based on computer-assisted image analysis and morphometric quantification of nuclear features have shown utility in differentiating benign and malignant aspirates. [ 5 6 7 8 ]…”

Section: Introductionmentioning

confidence: 99%

“…A large number of parameters have been assessed objectively by morphometry, but nuclear size parameters have been found to be significant in various studies. [ 5 6 7 8 9 ] These nuclear morphometric features have also been shown to be related to carcinoma grade and may predict the prognosis of the carcinoma patients. [ 10 ]…”

Section: Introductionmentioning

confidence: 99%

“…[ 11 ] Morphometric studies using freely available image analysis software may help in creating an effective low-cost automated screening system for breast aspirates. [ 7 8 9 10 ]…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Image Analysis-Assisted Nuclear Morphometric Study of Benign and Malignant Breast Aspirates

Pandian

Ramdas

Ambroise

2021

Journal of Microscopy and Ultrastructure

View full text Add to dashboard Cite

Background: Fineneedle aspiration cytology of the breast is well accepted and accurate for the diagnosis of benign and malignant lesions, however, it remains a subjective evaluation. Aims and Objectives: The present study was carried out to assess the utility of nuclear morphometry in differentiating benign and malignant breast aspirates. Importantly, we wanted to evaluate the utility of nuclear density parameters using Image J software. Materials and Methods: Nuclear morphometry was carried out using image analysis software Image J 1.44 on 20 selected benign and malignant breast aspirates. Assessment was carried out on a total of 1000 cells in benign and 1000 cells in malignant aspirates counting 50 intact nuclei in nonoverlapping cells for each case. Six parameters including three size parameters, namely nuclear area, nuclear diameter, and nuclear perimeter; one shape parameter, i.e., axis ratio; and two nuclear chromasia parameters, namely density (integrated and raw), were measured. Results: There were significant differences between nuclear area, perimeter, diameter, integrated density, and raw integrated density of benign and malignant lesions. No significant difference was found for axis ratio. Receiver operating characteristic curve analysis revealed that nuclear area, perimeter, diameter, integrated density, and raw integrated density are helpful in discriminating benign and malignant aspirates. Conclusions: Thus, Image J is helpful in the evaluation of nuclear size as well as chromasia. We conclude that nuclear size and density parameters can be used to derive cutoff values of various parameters to differentiate between benign and malignant cells in breast cytology.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…[ 11 ] Morphometric studies using freely available image analysis software may help in creating an effective low-cost automated screening system for breast aspirates. [ 7 8 9 10 ]…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Image Analysis-Assisted Nuclear Morphometric Study of Benign and Malignant Breast Aspirates

Pandian

Ramdas

Ambroise

2021

Journal of Microscopy and Ultrastructure

View full text Add to dashboard Cite

show abstract

“…Наші результати ядерних мофометричних параметрів відрізняються від описаних в літературі. Такі ж відмінності є і між результатами отриманими різними дослідниками при вивченні цих параметрів в новоутвореннях молочної залози жінок: 1) площа ядра у доброякісних пухлин 47,32 ± 3,53 мкм 2 , а у злоякісних 140,75 ± 22,94мкм 2 (Chowdhury et al, 2017); у доброякісних (лобулярна гіперплазія) 60,1 мкм 2 , а у злоякісних (внутріпротокова карцинома) 94,3 мкм 2 (Ikpatt et al, 2002); у доброякісних (гіперплазія) 30,62 мкм 2 , а у злоякісних (карцинома in situ) 45,21 мкм 2 (Ruiz et al, 1999); у доброякісних 38,34 ± 7,73 мкм 2 , а у злоякісних (карцинома in situ) 105,63 ± 17,45 мкм 2 (Radwan et al, 2003); у доброякісних 28,46 ± 7,72 мкм 2 , а у злоякісних 94,19 ± 19,49 мкм 2 (Kalhan et al, 2010); 2) периметр ядра 24,36 ± 0,916 мкм і 41,92 ± 3,33 мкм (Chowdhury et al, 2017); 28,9 мкм і 35,5 мкм (Ikpatt et al, 2002); 20,3 мкм і 28,88 мкм (Ruiz et al, 1999);25,08 ± 2,36 мкм і 40,73 ± 3,28 мкм (Radwan et al, 2003);19,77 ± 2,42 і 36,19 ± 4,91 (Kalhan et al, 2010); 3) діаметр ядра 7,74 мкм і 13,34 мкм (Chowdhury et al, 2018); 8,6 мкм і 10,7 мкм (Ikpatt et al, 2002). Ikpatt et al (2002) припускають, що різниця в морфометричних параметрах ядра новоутворень молочної залози жінок з різних країн може бути наслідком різ-них клінічних стадій захворювання та відмінностей в методах досліджень.…”

Section: рис 3 тубулопапілярна карцинома х400unclassified

Nuclear morphometry of mammary tumors of dogs and histopathological diagnosis

et al. 2020

View full text Add to dashboard Cite

For dog’s mammary tumors diagnostics scientists need researches, which can adopt and use methods developed for humans. The aim of this research paper is to set the inter relation between the parameters of nuclear morphometry (nuclear area, diameter, perimeter) and histopathological type of mammary tumors of dogs. Animals aged from 6 to 12 (medium meaning 9.2 ± 1.6 years). According to histopathological research 3 tumors were benign and 25 were malignant, 18 of them – malignant epithelial neoplasms (3 tubular carcinomas, 13 tubulopapillary carcinomas, 1 cystic-papillary carcinoma, 3 colid carcinomas, 1 micropapillary carcinoma), 3 malignant epithelial neoplasms of special type (mucinous, lipid-rich, spindle cell carcinomas) and 4 malignant mesenchymal neoplasms (chondrosarcoma). Nuclear morphometry parameters (nuclear area, perimeter and diameter) of benign tumours (20.48 ± 1.22, 19.13–21.50 µm2; 19.27 ± 0.10, 19.17–19.36 µm and 5.09 ± 0.16, 4.92–5.22 µm) were probably smaller than in malignant tumours, for example in simple carcinoma (38.61 ± 5.61, 29.26–46.16 µm2; 26.42 ± 2.32, 22.10–29.60 µm and 6.96 ± 0.52, 6.03–7.62 µm), tubular (37.89 ± 7.30, 29.94–46.16 µm2, 26.34 ± 2.83, 22,98–29,60 µm and 6.90 ± 0.70, 6.16–7.62 µm), tubulopapillary (40.22 ± 3.48, 34.38–44.75 µm2, 27.02 ± 1.49, 24.41–28.97 µm and 7.12 ± 0.31, 6.58–7.52 µm), colid (43.57 ± 5.54, 37.71–48.73 µm2, 28.05 ± 1.88, 26.54–30.15 µm and 7.41 ± 0.47, 6.91–7.85 µm), other malignant epithelial neoplasms (39.99 ± 5.15, 29.94–48.73 µm2; 26.85 ± 2.03, 22.98–30.15 µm and 7.09 ± 0.50, 6.16–7.85 µm) and malignant epithelial neoplasms of special types (45.89 ± 4.12, 43.41–50.65 µm2; 29.92 ± 0.21, 29.68–30.06 µm and 7.60 ± 0.34, 7.41–8.00 µm). However, there was not statistically significant difference in comparison between benign tumours and sarcomas (25.95 ± 5.21, 21.64–33.00 µm2; 21.85 ± 1.79, 20.21–24.05 µm and 5.68 ± 0.56, 5.21–6.42 µm). Among the different groups of malignant neoplasms lower rates were in sarcoma, the other groups had no difference. Taking into consideration the indicators of nuclear morphometry (nuclear area, diameter and perimeter) different types of neoplasms can be differentiated: benign from malignant tumours and sarcomas from malignant epithelial neoplasms (tubular, tubulopapillary, cystic-papillary, colid, micropapillary, mucinous, lipid-rich and spindle cell carcinomas).

show abstract

Role of Nuclear Morphometry as Objective Parameter to Evaluate Cytology Smears of Epithelial Breast Lesions

Cited by 2 publications

References 0 publications

Image Analysis-Assisted Nuclear Morphometric Study of Benign and Malignant Breast Aspirates

Image Analysis-Assisted Nuclear Morphometric Study of Benign and Malignant Breast Aspirates

Nuclear morphometry of mammary tumors of dogs and histopathological diagnosis

Contact Info

Product

Resources

About