Role of oocyte quality in meiotic maturation and embryonic development

Marteil, Gaëlle; Richard-Parpaillon, Laurent; Kubiak, Jacek Z.

doi:10.1016/s1642-431x(12)60027-8

Cited by 94 publications

(55 citation statements)

References 77 publications

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“…EP45 is probably not the only protein with Omen activity and numerous proteins acting in synergy might enhance M-phase entry. In oocytes, they could collectively contribute to the quality of oocytes, without individually affecting the core pathway leading to CDK1 activation (Marteil et al, 2009). We have recently found other potential Omen proteins as well as potential oocytematuration repressors (Omre) in additional proteomic screens (D'Inca et al, 2010).…”

Section: Ooocyte-maturation-enhancing Function Of Ep45mentioning

confidence: 99%

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EP45 accumulates in growingXenopus laevisoocytes and has oocyte-maturation-enhancing activity involved in oocyte quality

Marteil

d'Inca

Pascal

et al. 2010

Journal of Cell Science

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SummaryThe capacity of oocytes to fully support meiotic maturation develops gradually during oocyte growth. Growing oocytes accumulate proteins and mRNAs required for this process. However, little is known about the identity of these factors. We performed a differential proteomic screen comparing the proteomes of growing stage-IV oocytes, which do not undergo meiotic maturation in response to progesterone, with fully grown stage-VI ones, which do. In 2D gels of stage-VI oocytes, we identified a group of four protein spots as EP45 (estrogen-regulated protein 45 kDa), which belongs to the family of serine protease inhibitors and is also known as Seryp or pNiXa. Western blot analysis after mono-and bi-dimensional electrophoreses confirmed the accumulation of certain forms of this protein in oocytes between stages IV and VI. EP45 mRNA was not detectable in oocytes or ovaries, but was expressed in the liver. A low-mobility isoform of EP45 was detected in liver and blood, whereas two (occasionally three or four) higher-mobility isoforms were found exclusively in oocytes, suggesting that liver-synthesized protein is taken up by oocytes from the blood and rapidly modified. Alone, overexpression of RNA encoding either full-length or N-terminally truncated protein had no effect on meiotic resumption in stage-IV or -VI oocytes. However, in oocytes moderately reacting to low doses of progesterone, it significantly enhanced germinalvesicle breakdown, showing a novel and unsuspected activity of this protein. Thus, EP45 accumulates in growing oocytes through uptake from the blood and has the capacity to act as an 'oocyte-maturation enhancer' ('Omen').

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Section: Ooocyte-maturation-enhancing Function Of Ep45mentioning

confidence: 99%

“…Thus, the quality of mature oocytes depends largely on the quality of stockpiles accumulated during oogenesis. These processes are of key importance for embryonic developmental and reproductive success (Marteil et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

EP45 accumulates in growingXenopus laevisoocytes and has oocyte-maturation-enhancing activity involved in oocyte quality

Marteil

d'Inca

Pascal

et al. 2010

Journal of Cell Science

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“…According to the different stages of meiosis, oocytes are at three different phases of nuclear maturation, which are as follows: germinal vesicle (GV), metaphase I (MI), and MII (Cha & Chian 1998, Marteil et al 2009. At the end of the nuclear maturation process, the oocyte that is arrested at MII following the extrusion of the first polar body (PB) is considered mature and able to be fertilized by the sperm.…”

Section: Introductionmentioning

confidence: 99%

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

Huang¹,

Hao²,

Shen³

et al. 2013

Reproduction

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Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The abnormalities of endocrine and intra-ovarian paracrine interactions may change the microenvironment for oocyte development during the folliculogenesis process and reduce the developmental competence of oocytes in PCOS patients who are suffering from anovulatory infertility and pregnancy loss. In this microenvironment, the cross talk between an oocyte and the surrounding cumulus cells (CCs) is critical for achieving oocyte competence. The aim of our study was to investigate the gene expression profiles of CCs obtained from PCOS patients undergoing IVF cycles in terms of oocyte maturation by using human Genome U133 Plus 2.0 microarrays. A total of 59 genes were differentially expressed in two CC groups. Most of these genes were identified to be involved in one or more of the following pathways: receptor interactions, calcium signaling, metabolism and biosynthesis, focal adhesion, melanogenesis, leukocyte transendothelial migration, Wnt signaling, and type 2 diabetes mellitus. According to the different expression levels in the microarrays and their putative functions, six differentially expressed genes (LHCGR, ANGPTL1, TNIK, GRIN2A, SFRP4, and SOCS3) were selected and analyzed by quantitative RT-PCR (qRT-PCR). The qRT-PCR results were consistent with the microarray data. Moreover, the molecular signatures (LHCGR, TNIK, and SOCS3) were associated with developmental potential from embryo to blastocyst stage and were proposed as biomarkers of embryo viability in PCOS patients. Our results may be clinically important as they offer a new potential strategy for competent oocyte/embryo selection in PCOS patients.

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“…The competence of oocytes depends on numerous processes taking place during the whole oogenesis, which are age, body mass index (BMI), basal follicle stimulating hormone (FSH), and estradiol. 3 Estradiol production will increase along the follicle size growing and the oocyte become more mature. Hence, the higher level of estradiol could be the characteristic of oocyte maturation.…”

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confidence: 99%

Basal follicle stimulating hormone and leptin on the day of hCG administration predict successful fertilization in <em>in vitro</em> fertilization

2016

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ABSTRAK ABSTRACTBackground: Successful pregnancy in in vitro fertilization (IVF) program depends on multiple factors. This study aimed to determine whether age, body mass index (BMI), basal follicle stimulating hormone (FSH), estradiol, and leptin on the day of trigger ovulation with human chorionic gonadotropin (hCG) might be used as predictor for successful oocyte fertilization in in vitro fertilization (IVF) program.

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Role of oocyte quality in meiotic maturation and embryonic development

Cited by 94 publications

References 77 publications

EP45 accumulates in growingXenopus laevisoocytes and has oocyte-maturation-enhancing activity involved in oocyte quality

EP45 accumulates in growingXenopus laevisoocytes and has oocyte-maturation-enhancing activity involved in oocyte quality

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

Basal follicle stimulating hormone and leptin on the day of hCG administration predict successful fertilization in <em>in vitro</em> fertilization

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