Role of OsCZMT1 in Na+ and Mg2+ transport and salinity insensitivity

Lim, Sung Don; Kim, Jong Ho; Lee, Jeongeun; Hwang, Sun‐Goo; Shim, Su-Hyeon; Jeon, Jong‐Seong; Jang, Cheol Seong

doi:10.1016/j.envexpbot.2021.104754

Cited by 7 publications

(6 citation statements)

References 73 publications

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“…For instance, SOS1 ‐overexpressing transgenic plants showed significantly improved salt tolerance compared with WT plants (Yang et al, 2009). However, in previous studies, rice plants with salt‐tolerant genotypes showed lower expression levels of OsSOS1 than WT or control plants under salt stress (Lim et al, 2022; Park et al, 2019), which may be explained by the low accumulation of Na + in the cytoplasm of root cells under salt stress that restricts the transcript activation of OsSOS1 .…”

Section: Discussionmentioning

confidence: 90%

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Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na⁺ homeostasis in rice

Kim,

Lim,

Jung

et al. 2023

Physiologia Plantarum

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Soil salinity has a negative effect on crop yield. Therefore, plants have evolved many strategies to overcome decreases in yield under saline conditions. Among these, E3‐ubiquitin ligase regulates salt tolerance. We characterized Oryza sativa Really Interesting New Gene (RING) Finger C3HC4‐type E3 ligase (OsRFPHC‐4), which plays a positive role in improving salt tolerance. The expression of OsRFPHC‐4 was downregulated by high NaCl concentrations and induced by abscisic acid (ABA) treatment. GFP‐fused OsRFPHC‐4 was localized to the plasma membrane of rice protoplasts. OsRFPHC‐4 encodes a cellular protein with a C3HC4‐RING domain with E3 ligase activity. However, its variant OsRFPHC‐4C161A does not possess this activity. OsRFPHC‐4‐overexpressing plants showed enhanced salt tolerance due to low accumulation of Na+ in both roots and leaves, low Na+ transport in the xylem sap, high accumulation of proline and soluble sugars, high activity of reactive oxygen species (ROS) scavenging enzymes, and differential regulation of Na+/K+ transporter expression compared to wild‐type (WT) and osrfphc‐4 plants. In addition, OsRFPHC‐4‐overexpressing plants showed higher ABA sensitivity under exogenous ABA treatment than WT and osrfphc‐4 plants. Overall, these results suggest that OsRFPHC‐4 contributes to the improvement of salt tolerance and Na+/K+ homeostasis via the regulation of changes in Na+/K+ transporters.

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Section: Discussionmentioning

confidence: 90%

“…To visualize and identify the accumulation of Na + in rice root cells, Na + ‐specific fluorescent dye (Coro‐Na‐Green AM; Invitrogen) analysis was performed on the root tips of the WT, OsRFPHC‐4 ‐overexpressing, and osrfphc‐4 plants. Staining was performed as previously described (Lim et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na⁺ homeostasis in rice

Kim,

Lim,

Jung

et al. 2023

Physiologia Plantarum

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“…Mmembers of CorA superfamily also function in Mg 2+ dynamics ( Schock et al 2000 ; Li et al 2001 ; Gebert et al 2009 ). For the Mg 2+ uptake system, Arabidopsis MITOCHONDRIAL RNA SPLICING 2/MAGNESIUM TRANSPORTER 6 (AtMRS2–4/MGT6) ( Mao et al 2014 ), OsMGT1/OsMRS2-2 ( Chen et al 2017 ), and CorA-LIKE ZntB MAGNESIUM TRANSPORTER 1 (OsCZMT1) ( Lim et al 2022 ) are suggested to be involved, although the mechanistic basis for their function has not fully been elucidated. Keeping magnesium stored in the vacuole is another important strategy for surviving environmental fluctuations.…”

Section: Introductionmentioning

confidence: 99%

Mutations in RZF1, a zinc-finger protein, reduce magnesium uptake in roots and translocation to shoots in rice

et al. 2023

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Magnesium (Mg) homeostasis is critical for maintaining many biological processes, but little information is available to comprehend the molecular mechanisms regulating Mg concentration in rice (Oryza sativa). To make up for the lack of information, we aimed to identify mutants defective in Mg homeostasis through a forward genetic approach. As a result of the screening of 2,825 M2 seedlings mutated by ion-beam irradiation, we found a rice mutant that showed reduced Mg content in leaves and slightly increased Mg content in roots. Radiotracer 28Mg experiments showed that this mutant, named low magnesium content 1 (LMGC1), has decreased Mg2+ influx in the root and Mg2+ translocation from root to shoot. Consequently, LMGC1 is sensitive to the low Mg condition and prone to develop chlorosis in the young mature leaf. The MutMap method identified a 7.4-kbp deletion in the LMGC1 genome leading to a loss of two genes. Genome editing using CRISPR-Cas9 further revealed that one of the two lost genes, a gene belonging to the RanBP2-type zinc finger family that we named RanBP2-TYPE ZINC FINGER1 (OsRZF1), was the causal gene of the low-Mg phenotype. OsRZF1 is a nuclear protein and may have a fundamental role in maintaining Mg homeostasis in rice plants.

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“…Therefore, for high‐throughput screening of induced mutations, Targeting Induced Local Lesions In Genome (TILLING) is broadly regarded as a genetic strategy (McCallum et al, 2000). For example, approximately 15,000 M 3 TILLING rice populations have been developed using gamma‐ray irradiation, and M 10 rice mutants have been developed to screen for improved agronomic traits (Cho et al, 2010; Hwang et al, 2014; Lim et al, 2020; Lim et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Physio‐biochemical and molecular characterization of a rice drought‐insensitive TILLING line 1 (ditl1) mutant

Choi

Lee

Seo

et al. 2022

Physiologia Plantarum

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Drought stress is a major abiotic stress that limits rice yield. Therefore, the development of new varieties tolerant to drought stress is a high priority in breeding programs. In this study, 150 rice M10 mutant lines, previously developed using gamma‐ray irradiation, were used, and a drought‐insensitive rice mutant (ditl1) was selected by drought stress screening. The ditl1 mutant exhibited significantly decreased water loss, leaf curling, and H2O2 accumulation under drought stress. Chlorophyll leaching assay and toluidine blue staining suggested lower cuticle permeability in ditl1 mutants than in wild‐type (WT) plants. In addition, transmission electron microscopy revealed that ditl1 plants accumulated more cuticular wax on the epidermal surface. Whole‐genome resequencing analysis suggested that the deletion of a single nucleotide on the LOC_Os05g48260 gene, a putative ortholog of WSD1 (wax ester synthase/diacylglycerol O‐acyltransferase in Arabidopsis), maybe be the gene responsible for the drought insensitive phenotype of ditl1. The ditl1 mutant will be a valuable breeding resource for developing drought stress tolerant rice cultivar.

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Role of OsCZMT1 in Na+ and Mg2+ transport and salinity insensitivity

Cited by 7 publications

References 73 publications

Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na⁺ homeostasis in rice

Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na⁺ homeostasis in rice

Mutations in RZF1, a zinc-finger protein, reduce magnesium uptake in roots and translocation to shoots in rice

Physio‐biochemical and molecular characterization of a rice drought‐insensitive TILLING line 1 (ditl1) mutant

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Role of OsCZMT1 in Na+ and Mg2+ transport and salinity insensitivity

Cited by 7 publications

References 73 publications

Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na+ homeostasis in rice

Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na+ homeostasis in rice

Mutations in RZF1, a zinc-finger protein, reduce magnesium uptake in roots and translocation to shoots in rice

Physio‐biochemical and molecular characterization of a rice drought‐insensitive TILLING line 1 (ditl1) mutant

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Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na⁺ homeostasis in rice

Overexpression of a C3HC4‐type E3‐ubiquitin ligase contributes to salinity tolerance by modulating Na⁺ homeostasis in rice