Role of Oxidative DNA Damage and Repair in Atrial Fibrillation and Ischemic Heart Disease

Hu, Liangyu; Wang, Zhengkun; Carmone, Claudia; Keijer, Jaap; Zhang, Deli

doi:10.3390/ijms22083838

Cited by 29 publications

(31 citation statements)

References 223 publications

(280 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Esters of 6-Hydroxy-flavanone and 7-Hydroxy-flavone 3.2.1. General Procedure for 6-Hydroxy-flavanone Esters of (3,5,7,9,11) Synthesis To a magnetically stirred at 22-23 • C solution of (±) 6-hydroxy-flavanone (0.24 g; 1.0 mmol) in dichloromethane:tetrahydrofuran mixture (10 mL:5 mL), pyridine (0.20 mL; 2.5 mmol) and next a solution of acyl chloride (2.0 mmol) in 2 mL of dichloromethane were added. The resulting solution was stirred at 22-23 • C for 16 h and concentrated under reduced pressure.…”

Section: Apparatus Materials and Analysismentioning

confidence: 99%

See 1 more Smart Citation

Effect of Hydroxyl Groups Esterification with Fatty Acids on the Cytotoxicity and Antioxidant Activity of Flavones

et al. 2022

View full text Add to dashboard Cite

Flavonoids and polyunsaturated fatty acids due to low cytotoxicity in vitro studies are suggested as potential substances in the prevention of diseases associated with oxidative stress. We examined novel 6-hydroxy-flavanone and 7-hydroxy-flavone conjugates with selected fatty acids (FA) of different length and saturation and examined their cytotoxic and antioxidant potential. Our findings indicate that the conjugation with FA affects the biological activity of both the original flavonoids. The conjugation of 6-hydroxy-flavanone increased its cytotoxicity towards prostate cancer PC3 cells. The most noticeable effect was found for oleate conjugate. A similar trend was observed for 7-hydroxy-flavone conjugates with the most evident effect for oleate and stearate. The cytotoxic potential of all tested conjugates was not specific towards PC3 because the viability of human keratinocytes HaCaT cells decreased after exposure to all conjugates. Additionally, we showed that esterification of the two flavonoids decreased their antioxidant activity compared to that of the original compounds. Of all the tested compounds, only 6-sorbic flavanone showed a slight increase in antioxidant potential compared to that of the original compound. Our data show that conjugated flavonoids are better absorbed and enhance cytotoxic effects, but the presence of FA lowered the antioxidant potential.

show abstract

Section: Apparatus Materials and Analysismentioning

confidence: 99%

“…They can interact with nucleic acids, proteins and lipids leading to severe metabolic disturbances. ROS have been implicated in the pathogenesis of a wide variety of diseases, including cancer, neurodegenerative diseases, metabolic diseases, cardiovascular diseases, respiratory diseases or rheumatoid arthritis [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Effect of Hydroxyl Groups Esterification with Fatty Acids on the Cytotoxicity and Antioxidant Activity of Flavones

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Based on these observations, we postulate that deficiency of NAD+ due to increased PARP activation may be a key factor related to the SARS-CoV-2 associated disease spectrum. Oxidative stress induces PARP1, whose hyper activation depletes NAD+ and ATP levels, culminating in energy loss and subsequent cell death [49][50][51]. Overall, these processes enhance pro-inflammatory signaling.…”

Section: Parp-1 In Viral Pathologymentioning

confidence: 99%

Therapeutic Significance of microRNA-Mediated Regulation of PARP-1 in SARS-CoV-2 Infection

Dash

Pandhare

2021

ncRNA

View full text Add to dashboard Cite

The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 (2019-nCoV) has devastated global healthcare and economies. Despite the stabilization of infectivity rates in some developed nations, several countries are still under the grip of the pathogenic viral mutants that are causing a significant increase in infections and hospitalization. Given this urgency, targeting of key host factors regulating SARS-CoV-2 life cycle is postulated as a novel strategy to counter the virus and its associated pathological outcomes. In this regard, Poly (ADP)-ribose polymerase-1 (PARP-1) is being increasingly recognized as a possible target. PARP-1 is well studied in human diseases such as cancer, central nervous system (CNS) disorders and pathology of RNA viruses. Emerging evidence indicates that regulation of PARP-1 by non-coding RNAs such as microRNAs is integral to cell survival, redox balance, DNA damage response, energy homeostasis, and several other cellular processes. In this short perspective, we summarize the recent findings on the microRNA/PARP-1 axis and its therapeutic potential for COVID-19 pathologies.

show abstract

“…To better characterize the molecular and 14 Oxidative Medicine and Cellular Longevity cellular events related to AKI, we used the TRAP-seq results in the GEO database to examine PLK3 expression in the translation profile of nephrons (tubules) exposed to IRI for 24 h. GO analysis showed that PLK3 was mainly involved in oxidative stress and DNA damage after renal I/R injury. DNA damage has been reported to be involved in I/R injury in a variety of vital organs, including the testicular, brain, liver, and heart [33][34][35][36]. During renal I/R injury, DNA fragmentation in the renal tubules after DNA damage occurs as early as 12 h after reperfusion and increases within 24 h [37].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of PLK3 Attenuates Tubular Epithelial Cell Apoptosis after Renal Ischemia–Reperfusion Injury by Blocking the ATM/P53-Mediated DNA Damage Response

Deng

Wei

Xie

et al. 2022

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Objective. Renal ischemia–reperfusion (I/R) injury is a major cause of acute kidney injury (AKI) in transplanted kidneys. This study was aimed at exploring the role of PLK3 (polo-like kinase 3) in renal I/R injury, focusing on its relationship with oxidative stress-induced DNA damage and renal tubular epithelial cell (TEC) apoptosis. Methods. TRAP-seq data from the development dataset GSE52004 and the validation dataset GSE121191 were analyzed using GEO2R. PLK3 overexpression plasmids and targeted silencing siRNAs were used in a model of hypoxia/reoxygenation (H/R) injury, and rAAV-9-PLK3-KD were administered to C57BL/6J mice exposed to I/R injury. The ATM-specific inhibitor KU-60019 was used to block the DNA damage response (DDR). Western blotting was performed to measure DDR- and apoptosis-associated protein expression. Cell viability was measured by CCK-8 reagent, and apoptosis was examined by flow cytometry and TUNEL assay. Furthermore, the fluorescent probes H2DCFH-DA and DHE were used to measure ROS production in vitro. The MDA level and SOD activity were measured to assess oxidative stress in vivo. KIM-1 staining and Scr and BUN were used to evaluate kidney injury. Results. The mRNA and protein levels of PLK3 were markedly increased in the H/R injury and I/R injury models. GO terms showed that PLK3 was mainly involved in oxidative stress and DNA damage after renal I/R injury. Overexpression of PLK3 decreased cell viability and increased apoptosis. In contrast, targeted silencing of PLK3 expression decreased the Bax/Bcl-2 ratio by decreasing P53 phosphorylation, thereby reducing TEC apoptosis. Furthermore, KU-60019 reduced PLK3 activation and DDR-induced apoptosis, while overexpression of PLK3 reversed the mitigating effect of KU-60019 on TEC apoptosis. Similarly, rAAV-9-PLK3 KD mice exhibited a lower rate of TEC apoptosis and milder renal damage after I/R injury. Conclusion. We demonstrate for the first time that PLK3 is involved in oxidative stress-induced DNA damage and TEC apoptosis in renal I/R injury. Inhibition of PLK3 attenuates TEC apoptosis after I/R injury by blocking the ATM/P53-mediated DDR. Therefore, PLK3 may serve as a potential therapeutic target for ischemic AKI.

show abstract

Role of Oxidative DNA Damage and Repair in Atrial Fibrillation and Ischemic Heart Disease

Cited by 29 publications

References 223 publications

Effect of Hydroxyl Groups Esterification with Fatty Acids on the Cytotoxicity and Antioxidant Activity of Flavones

Effect of Hydroxyl Groups Esterification with Fatty Acids on the Cytotoxicity and Antioxidant Activity of Flavones

Therapeutic Significance of microRNA-Mediated Regulation of PARP-1 in SARS-CoV-2 Infection

Inhibition of PLK3 Attenuates Tubular Epithelial Cell Apoptosis after Renal Ischemia–Reperfusion Injury by Blocking the ATM/P53-Mediated DNA Damage Response

Contact Info

Product

Resources

About