The importance of biomarker quantification in technology cannot be overstated. It has numerous applications in medical diagnostics, drug delivery, and the timely implementation of prevention and control strategies for highly prevalent diseases worldwide. However, the discovery of new tools for detection has become increasingly necessary. One promising avenue is the use of perovskite-based materials, which exhibit excellent catalytic activity and redox properties. These make them ideal candidates for the development of electrochemical sensors. In this review, the advances of purely non-enzymatic electrochemical detection of bio-analytes, with ABO3 perovskite form, are presented. The work allows the visualization of some of the modifications in the composition and crystal lattice of the perovskites and some variations in the assembly of the electrodes, which can result in systems with a better response to the detection of analytes of interest. These findings have significant implications for improving the accuracy and speed of biomarker detection, ultimately benefiting patients and healthcare professionals alike.