Role of oxygen supply in submerged fermentation of Ganoderma lucidum for production of Ganoderma polysaccharide and ganoderic acid

Tang, Ya‐Jie; Zhong, Jian‐Jiang

doi:10.1016/s0141-0229(02)00338-1

Cited by 129 publications

(72 citation statements)

References 16 publications

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“…In solid-state fermentation of Monascus purpureus, Han and Mudgett [8] reported that oxygen enrichment to the gas environment stimulated pigment production. Although oxygen supply and dissolved oxygen level in liquid affected the mycelia growth and GA accumulation by G. lucidum in stirred fermentors [9], the effect of oxygen levels in the gaseous phase on the growth and GA production remained unknown, not to mention the liquid static culture.…”

Section: Introductionmentioning

confidence: 99%

Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum

Zhang

Tang

Zhong

2009

Bioprocess Biosyst Eng

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Liquid static cultivation of Ganoderma lucidum was previously found to be very efficient for improving the production of its valuable antitumor compound ganoderic acid (GA) (Fang and Zhong in Biotechnol Prog 18:51-54, 2002). In this work, effects of oxygen concentration within the range of 21-100% (v/v) in the gaseous phase on the mycelia growth, GA production, and gene transcription of key enzymes for GA biosynthesis in liquid static cultures of G. lucidum were investigated. A high cell density of 29.8 +/- 1.7 g/l DW and total GA production of 1427.2 +/- 74.2 mg/l were obtained under an optimal gaseous O(2) level of 80%. The expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase, squalene synthase and lanosterol synthase genes of GA biosynthetic pathway as detected by quantitative real-time PCR was also affected by the gaseous oxygen concentration in the liquid static culture. H(2)O(2) was generated as reactive oxygen species in response to high oxygen concentrations in the gas phase, and it seemed to be involved in the regulation of GA biosynthesis. The information obtained in this study provided an insight into the role of gaseous O(2) in the GA production and it will be helpful for further enhancing its productivity.

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Section: Introductionmentioning

confidence: 99%

Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum

Zhang

Tang

Zhong

2009

Bioprocess Biosyst Eng

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“…To accelerate BIO and EPS productions, many studies have been conducted to examine the influence of operation conditions on G. lucidum culture, including medium composition [7][8][9][10][11][12], inoculation density [13], pH [14,15], temperature [7,16], oxygen supply [17], and even cultivation process variation [18]. Furthermore, some reports have emphasized the stimulating effects on BIO and EPS productions by the addition of plant oils.…”

Section: Introductionmentioning

confidence: 99%

Experimental analysis of the oil addition effect on mycelia and polysaccharide productions in Ganoderma lucidum submerged culture

Huang

Chen

Hung

et al. 2008

Bioprocess Biosyst Eng

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The effect of corn oil addition on mycelium growth and polysaccharide productions in the medicinal mushroom Ganoderma lucidum was studied. The results showed that when a level of 2% corn oil was added at the beginning of culture, the biomass and polysaccharide productions reached a maximum of 12.9 and 1.038 g/L, respectively, during 13-day cultivation. The pH variation along with morphology observation in culture provided an indirect inference to the promotional effect of oil addition. Moreover, a curve fitting analysis was carried out to assay the elevated effect on biomass and exopolysaccharide productions in oil added culture. The experimental data of substrates consumption and products formation in culture with oil addition were predicted through the fitting equations obtained in single carbon source culture. The numerical results further clarified the stimulatory effects of oil addition in G. lucidum culture.

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“…31,38) Although growing G. lucidum on PDA markedly enhanced the production of GAs, PDA did not support high levels of intracellular polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Production of Ganoderic Acids and Cytotoxicity ofGanoderma lucidumUsing Solid-Medium Culture

You

Lee

Chung

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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Role of oxygen supply in submerged fermentation of Ganoderma lucidum for production of Ganoderma polysaccharide and ganoderic acid

Cited by 129 publications

References 16 publications

Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum

Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum

Experimental analysis of the oil addition effect on mycelia and polysaccharide productions in Ganoderma lucidum submerged culture

Enhanced Production of Ganoderic Acids and Cytotoxicity ofGanoderma lucidumUsing Solid-Medium Culture

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