Role of PDGF-BB in proliferation, differentiation and maintaining stem cell properties of PDL cells in vitro

Mihaylova, Zornitsa; Tsikandelova, Rozaliya; Sanimirov, Pavel; Gateva, Natalia; Mitev, Vanio; Ishkitiev, Nikolay

doi:10.1016/j.archoralbio.2017.09.019

Cited by 42 publications

(37 citation statements)

References 40 publications

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“…Taking these in consideration, we would expect in our future 3D model that 25 ng/ml PDGF addition would not harm vessel formation, although it should be therapeutically active on hMSC and, potentially, promote their role in vessel stabilisation. Indeed, it has been shown previously that PDGF upregulated hMSC metabolic activity and proliferation (Chase, Lakshmipathy, Solchaga, Rao, & Vemuri, ; Fierro et al, ; Mihaylova et al, ). The timepoint chosen for the 2D studies was based on previously published data (Chase et al, ); however, it is possible that additional effects of PDGF could be identified in these cells at other timepoints.…”

Section: Discussionmentioning

confidence: 88%

Platelet‐derived growth factor stabilises vascularisation in collagen‐glycosaminoglycan scaffolds in vitro

Amaral

Cavanagh

O’Brien

et al. 2018

J Tissue Eng Regen Med

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Collagen-glycosaminoglycan (CG) scaffolds have been widely developed for a range of regenerative medicine applications. To enhance their efficacy, CG scaffolds have previously been prevascularised in vitro using human umbilical vein endothelial cells and human mesenchymal stromal cells (hMSCs); however, at later timepoints, a regression of vascularisation is observed. This is undesirable for longer preculture periods (e.g., for partial/full organ regeneration) and for in vitro vascularised tissue model systems (e.g., for drug testing/modelling). We hypothesised that delayed platelet-derived growth factor (PDGF)-BB addition could stabilise vessels, preventing their regression. In 2D, we identified 25 ng/ml as a suitable dose that enhanced hMSC metabolic activity and proliferation, without affecting endothelial cells, or migration in either cell type. In our 3D model of CG scaffold vascularisation, early addition of

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Section: Discussionmentioning

confidence: 88%

Platelet‐derived growth factor stabilises vascularisation in collagen‐glycosaminoglycan scaffolds in vitro

Amaral

Cavanagh

O’Brien

et al. 2018

J Tissue Eng Regen Med

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“…We used two murine strains of MSCs in the current study to investigate the co-expression GM MSCs. It is been reported that PDGF-BB enhanced the proliferation of several cell types including endothelial cells [6] and populations of MSCs [31,32]. By studying the cell proliferation and cell viability, the results showed that the presence of PDGF-BB from GM MSCs or exogenous PDGF-BB protein in BALB/c MSCs increased cell proliferation and cell viability.…”

Section: Discussionmentioning

confidence: 90%

“…Recombinant human PDGF-BB (rhPDGF-BB) in combination with an osteoinductive tricalcium phosphate (TCP)-collagen matrix has been applied clinically in ankle and hindfoot arthrodesis, and was shown to be a safe and effective alternative to autograft [33,34]. In vitro and in vivo studies have previously demonstrated the capacity for PDGF-BB to facilitate osteogenesis by stem cells [7,31,32]. Furthermore, PDGF-BB secreted by preosteoclasts induced angiogenesis during coupling with osteogenesis [6].…”

Section: Discussionmentioning

confidence: 99%

PDGF-BB and IL-4 co-overexpression is a potential strategy to enhance mesenchymal stem cell-based bone regeneration

Zhang

Utsunomiya

et al. 2020

Preprint

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Background Mesenchymal stem cell (MSC)-based therapy has the potential for immunomodulation and enhancement of tissue regeneration. Genetically modified (GM) MSCs that over-express specific cytokines, growth factors or chemokines have shown great promise in pre-clinical studies. In this regard, the anti-inflammatory cytokine Interleukin (IL)-4 converts pro-inflammatory M1 macrophages into an anti-inflammatory M2 phenotype; M2 macrophages could potentially enhance osteogenesis by MSC lineage cells. However, IL-4 inhibits osteogenesis during the acute inflammatory period. Platelet-derived growth factor (PDGF)-BB has been shown to enhance osteogenesis of MSCs with a dose-dependent effect. Methods In this study, we generated a lentiviral vector that produces PDGF-BB under a weak promoter (phosphoglycerate kinase, PGK) and lentiviral vector producing IL-4 under a strong promoter (cytomegalovirus, CMV). We infected the MSCs with the PDGF-BB and IL-4-producing lentiviral vectors separately or in combination to investigate protein expression, cell proliferation and viability as well as the capability for osteogenesis. Results Co-overexpression of PDGF-BB and IL-4 was observed in the co-infected MSCs and shown to enhance cell proliferation and viability, and osteogenesis compared to IL-4 overexpressing MSCs alone. Conclusions Thus, overexpression of both PDGF-BB and IL-4 may be a potential strategy to facilitate osteogenesis in scenarios of both acute and chronic inflammation.

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“…Periodontal MSCs are isolated from the middle third of the root of permanent teeth and also from the walls of the dental socket after tooth extraction [3]. After scraping with sterile scalpel blades, the tissue explants are incubated in enzymatic solutions to obtain a single-cell suspension [4,5,6]. The resulting suspension is seeded in plastic cell culture dishes.…”

Section: Isolation Of Mscs From Human Pdlmentioning

confidence: 99%

Periodontal ligament stem cells-current review of the literature

Aleksiev

Ugrinov

Hristov

2019

ijmsci

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The presence of mesenchymal stem cells (MSCs) in human periodontal ligament (PDL) were first described by Seo et al. in 2004. These cells are capable of plastic adherence, colony forming and differentiation into various cell types when cultivated in appropriate cell culture conditions. PDL stem cells are investigated by high number of scientists nowadays because of their high potency for tissue regeneration. However, little is known about the effects of various active molecules and growth factors on their stem cell properties. There is limited number of reports revealing the activity of these cells when applied in clinical studies. The purpose of the current review is to highlight the stem cell properties of human PDL MSCs and to briefly systematize the data from various sources.

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Role of PDGF-BB in proliferation, differentiation and maintaining stem cell properties of PDL cells in vitro

Cited by 42 publications

References 40 publications

Platelet‐derived growth factor stabilises vascularisation in collagen‐glycosaminoglycan scaffolds in vitro

Platelet‐derived growth factor stabilises vascularisation in collagen‐glycosaminoglycan scaffolds in vitro

PDGF-BB and IL-4 co-overexpression is a potential strategy to enhance mesenchymal stem cell-based bone regeneration

Periodontal ligament stem cells-current review of the literature

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