Role of peri-axonal inflammation in the development of thermal hyperalgesia and guarding behavior in a rat model of neuropathic pain

Clatworthy, Andrea L.; Illich, Paul A.; Castro, Gilbert A.; Walters, Edgar T.

doi:10.1016/0304-3940(94)11154-b

Cited by 200 publications

(86 citation statements)

References 15 publications

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“…In addition, increased sensitivity of the affected paw to pressure or flexion and extension of the inflamed joints and weight loss were observed. The arthritis observed in rats is associated with a hyperalgesia phenomenon and spontaneous behaviors, such as protection of the affected paw, evidenced by curving and/or elevation of the paw, as well as avoidance of supporting the body on the paw (Clatworthy et al, 1995). The hyperalgesia is more evident during the acute inflammatory phase, when spontaneous behaviors, indicative of painful response are more pronounced.…”

Section: Resultsmentioning

confidence: 99%

Effect ofIndigofera aspalathoideson complete Freund’s adjuvant-induced arthritis in rats

Balasubramanian¹,

Kavimani²,

Ravichandiran

et al. 2009

Pharmaceutical Biology

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Section: Resultsmentioning

confidence: 99%

Effect ofIndigofera aspalathoideson complete Freund’s adjuvant-induced arthritis in rats

Balasubramanian¹,

Kavimani²,

Ravichandiran

et al. 2009

Pharmaceutical Biology

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“…A subpopulation of 5-HT 2B R-positive immunelike cells with no labeling for macrophage markers was also observed in the sciatic nerve after CCI, suggesting that other blood-derived cells express this receptor. They may correspond to neutrophils involved in the very early stages following peripheral nerve injury [14,48], with a decrease in infiltration by these cells attenuating mechanical allodynia [25]. Other immune cells express the receptor [1], including T lymphocytes [56].…”

Section: Discussionmentioning

confidence: 99%

Antinociceptive effect of peripheral serotonin 5-HT2B receptor activation on neuropathic pain

Urtikova

Berson

Steenwinckel

et al. 2012

Pain

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-nociceptive effect of peripheral serotonin 5-HT2B receptor activation on neuropathic pain.. PAIN, Elsevier, 2012, 153 (6), pp.1320-31. <10.1016/j.pain.2012.03.024>. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 receptor. CCI resulted in a biphasic upregulation of 5-HT 2B receptor expression in lumbar dorsal root ganglia, consisting of a transient early increase (23-fold), two days after surgery, before the development of neuropathic pain, followed by a steady, five times increase, levels remaining constant thereafter until the pain disappeared. 5-HT 2B receptors were immmunolocalized mostly on primary sensory neurons and infiltrating macrophages. Intrathecal injection of RS127445, a selective 5-HT 2B receptor antagonist, enhanced mechanical and cold allodynia. A single application of BW732C86, a 5-HT 2B agonist, to the sciatic nerve immediately after ligature completely prevented mechanical allodynia and significantly decreased cold allodynia. This effect was dose-dependent and reversed by a 5-HT 2B antagonist. We also observed a marked decrease in macrophage infiltration of the sciatic nerve, neuropathic pain markers and cytokine induction. Our data reveal the relationships between serotonin, the immune system and neuropathic pain, and demonstrate a critical role of 5-HT 2Breceptors in blood-derived macrophages.

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“…11,30 In Wallerian-degenerated nerves, macrophages and Schwann cells increase. 8,[31][32][33] Infiltration of macrophages in the experimental neuroma might promote neuropathic pain.…”

Section: Discussionmentioning

confidence: 99%

The autotomy relief effect of a silicone tube covering the proximal nerve stump

et al. 2006

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A technique of covering the proximal nerve stump (PNS) has been reported as a preventative method or treatment for neuroma. However, its detailed pain relief mechanism remains unknown. We created a silicone tube model in which the PNS of the rat sciatic nerve was introduced into the tube, whereas the controls had no tube. The score of autotomy observed in the tube group was lower than that in the control group at 3 days to 2 weeks after surgery, which suggested that the silicone tube had pain-like behavior inhibitory action. To elucidate the mechanism of autotomy inhibition, immunohistochemistry and Toluidine blue staining were performed in the PNS. The increase in S-100-immunoreactivity (IR) including Schwann cells was inhibited at 1 week after surgery in the tube group, and the increase in the number of macrophages shown by ED-1-IR at 1, 2, and 4 weeks after surgery was similarly inhibited. Toluidine blue staining showed that the increase in the number of mast cells was inhibited at 1, 2, and 4 weeks after surgery and in the number of lymphocytes at 1 and 2 weeks after surgery in the tube group. Therefore, blocking of the infiltration of inflammatory cells into the PNS by the silicone tube was thought to be the mechanism of autotomy inhibition. To further explore details of this mechanism, the expression of nerve growth factor (NGF), production of which is induced by inflammatory cells and of the NGF receptor TrkA was examined in the PNS and the dorsal root ganglion using immunohistochemistry and a ribonuclease protection assay. In the PNS, the increase in NGF-IR was inhibited at 1, 2, and 4 weeks after surgery in the tube group, suggesting that this could be one of the pain-like behavior inhibitory effects. ß

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Role of peri-axonal inflammation in the development of thermal hyperalgesia and guarding behavior in a rat model of neuropathic pain

Cited by 200 publications

References 15 publications

Effect ofIndigofera aspalathoideson complete Freund’s adjuvant-induced arthritis in rats

Effect ofIndigofera aspalathoideson complete Freund’s adjuvant-induced arthritis in rats

Antinociceptive effect of peripheral serotonin 5-HT2B receptor activation on neuropathic pain

The autotomy relief effect of a silicone tube covering the proximal nerve stump

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