Role of peroxisomes in the oxidative injury induced by 2,4-dichlorophenoxyacetic acid in leaves of pea plants

McCarthy-Suárez, Iva; Gómez, M. A.; Rı́o, Luis A. del; Palma, José M.

doi:10.1007/s10535-011-0114-7

Cited by 28 publications

(12 citation statements)

References 56 publications

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“…Previously, the peroxisomal enzyme XOD (Sandalio et al 1988) was demonstrated to be involved in ROS production induced by 2,4-D (Romero-Puertas et al 2004a;McCarthy-Su arez et al 2011;Pazmiño et al 2011). Analysis of XOD activity with native PAGE and spectrophotometry showed an increase in XOD activity with 2,4-D and a change in mobility of the protein in pea leaves treated with 2,4-D (Fig.…”

Section: Regulation Of Ros Production and Ethylene Emission By 24-dmentioning

confidence: 87%

“…In animal tissue, it has been demonstrated that XOD can be phosphorylated (Kayyali et al 2001), although the effect of this modification on XOD activity has not been studied. Recently, we detected an increase in this activity in pea plants exposed to different concentrations of 2,4-D (McCarthy-Su arez et al 2011;Pazmiño et al 2011). Acyl CoA oxidase (ACX) from the fatty acid b-oxidation pathway is also stimulated in response to 2,4-D treatment (McCarthy-Su arez et al 2011;Pazmiño et al 2011) and could also contribute to ROS production.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we detected an increase in this activity in pea plants exposed to different concentrations of 2,4-D (McCarthy-Su arez et al 2011;Pazmiño et al 2011). Acyl CoA oxidase (ACX) from the fatty acid b-oxidation pathway is also stimulated in response to 2,4-D treatment (McCarthy-Su arez et al 2011;Pazmiño et al 2011) and could also contribute to ROS production. Tobacco plants over-expressing aPPAR from Xenopus laevis showed induction of ACX activity and a curly phenotype similar to the epinasty induced by 2,4-D (Nila et al 2006).…”

Section: Discussionmentioning

confidence: 99%

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Regulation of epinasty induced by 2,4‐dichlorophenoxyacetic acid in pea and Arabidopsis plants

et al. 2014

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The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) causes uncontrolled cell division and malformed growth in plants, giving rise to leaf epinasty and stem curvature. In this study, mechanisms involved in the regulation of leaf epinasty induced by 2,4-D were studied using different chemicals involved in reactive oxygen species (ROS) accumulation (diphenyleniodonium, butylated hydroxyanisole, EDTA, allopurinol), calcium channels (LaCl3), protein phosphorylation (cantharidin, wortmannin) and ethylene emission/perception (aminoethoxyvinyl glycine, AgNO3). The effect of these compounds on the epinasty induced by 2,4-D was analysed in shoots and leaf strips from pea plants. For further insight into the effect of 2,4-D, studies were also made in Arabidopsis mutants deficient in ROS production (rbohD, rbohF, xdh), ethylene (ein 3-1, ctr 1-1, etr 1-1), abscisic acid (aba 3.1), and jasmonic acid (coi 1.1, jar 1.1, opr 3) pathways. The results suggest that ROS production, mainly ·OH, is essential in the development of epinasty triggered by 2,4-D. Epinasty was also found to be regulated by Ca2+, protein phosphorylation and ethylene, although all these factors act downstream of ROS production. The use of Arabidopsis mutants appears to indicate that abscisic and jasmonic acid are not involved in regulating epinasty, although they could be involved in other symptoms induced by 2,4-D.

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Section: Regulation Of Ros Production and Ethylene Emission By 24-dmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Regulation of epinasty induced by 2,4‐dichlorophenoxyacetic acid in pea and Arabidopsis plants

et al. 2014

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“…Pea plants exposed to 2,4‐D also develop oxidative stress symptoms characterized by H 2 O 2 over‐accumulation, lipid peroxidation, protein oxidation and induction of proteolysis (McCarthy 2004; Romero‐Puertas et al . 2004a; McCarthy‐Suárez et al . 2011).…”

Section: Introductionmentioning

confidence: 99%

Differential response of young and adult leaves to herbicide 2,4‐dichlorophenoxyacetic acid in pea plants: role of reactive oxygen species

Pazmiño

Rodríguez‐Serrano

Romero‐Puertas

et al. 2011

Plant Cell & Environment

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In this work the differential response of adult and young leaves from pea (Pisum sativum L.) plants to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) (23 mm) applied by foliar spraying was investigated. The concentration of 2,4-D (23 mm) and the time of treatment (72 h) were previously optimized in order to visualize its toxic effects on pea plants. Under these conditions, the herbicide induced severe disturbances in mesophyll cells structure and proliferation of vascular tissue in young leaves and increased acyl-CoA oxidase (ACX), xanthine oxidase (XOD) and lipoxygenase (LOX) activities in young leaves, and only ACX and LOX in adult leaves. This situation produced reactive oxygen species (ROS) over-accumulation favoured by the absence of significant changes in the enzymatic antioxidants, giving rise to oxidative damages to proteins and membrane lipids. An increase of ethylene took place in both young and adult leaves and the induction of genes encoding the stress proteins, PRP4A and HSP 71,2, was observed mainly in young leaves. These results suggest that ROS overproduction is a key factor in the effect of high concentrations of 2,4-D, and ROS can trigger a differential response in young and adult leaves, either epinasty development in young leaves or senescence processes in adult tissues.

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“…In the cells of higher plants, NADP-ICDH activity has been detected and characterized in the different subcellular compartments including the cytosol, mitochondria, chloroplasts and peroxisomes (Attucci et al 1994, Rasmusson and Møller 1998, Corpas et al 1999b, Leterrier et al 2007, Pascual et al 2008, McCarthy-Suárez et al 2011). Significant progress is being achieved in understanding how the NADP-ICDH is involved in nitrogen metabolism, in redox regulation, and in response to oxidative stress (Scheible et al 2000, Hodges et al 2003, Valderrama et al 2006, Mateos et al 2009, Mhamdi et al 2010, Sulpice et al 2010.…”

Section: Introductionmentioning

confidence: 99%

Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots

Leterrier¹,

Barroso²,

Palma³

et al. 2012

Biologia plant.

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NADP-dependent isocitrate dehydrogenase (NADP-ICDH) catalyses the production of NADPH, which is an essential component in the cellular homeostasis. In Arabidopsis, the kinetic parameters (K m and V max ) of cytosolic NADP-ICDH were different in leaves and roots. In vitro applied H 2 O 2 did not affect the NADP-ICDH activity in either organ, however, the reduced glutathione inhibited the activity in leaves but not in roots. On the other hand, S-nitrosoglutathione (a NO donor) and peroxynitrite depressed NADP-ICDH activity in leaves and roots.Additional key words: hydrogen peroxide, nitric oxide, peroxynitrite, reactive nitrogen species, S-nitrosoglutathione.

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Role of peroxisomes in the oxidative injury induced by 2,4-dichlorophenoxyacetic acid in leaves of pea plants

Cited by 28 publications

References 56 publications

Regulation of epinasty induced by 2,4‐dichlorophenoxyacetic acid in pea and Arabidopsis plants

Regulation of epinasty induced by 2,4‐dichlorophenoxyacetic acid in pea and Arabidopsis plants

Differential response of young and adult leaves to herbicide 2,4‐dichlorophenoxyacetic acid in pea plants: role of reactive oxygen species

Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots

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