The paper focuses on staff number reducing in Czech organizations because it is an important issue due to adaptation to changing economic conditions and ongoing financial crisis. The number of organizations reducing their staff number goes up in all sectors of economy including agriculture and the problem is associated with financial costs. The objective is to present the ways of staff number reduction in Czech organizations and outplacement for the laid-off workers and a partial objective is to compare the results with those in the Slovak Republic. Moreover, the paper discusses the recommendation in the sphere of knowledge continuity for organizations and it also examines the cost level of employees' turnover. The results were obtained by implementing quantitative research with the help of questionnaire data collection (n = 109) which were analyzed by the tools of descriptive statistics. Results show that 52.3% of organizations have reduced their staff number. However, outplacement was used by only 10.1% of the addressed Czech organizations, out of which 28.6% were agricultural.