Role of pH in the recovery of bovine milk oligosaccharides from colostrum whey permeate by nanofiltration

Cohen, Joshua L.; Barile, Daniela; Liu, Yan; Bell, Juliana Maria Leite Nóbrega de Moura

doi:10.1016/j.idairyj.2016.10.016

Cited by 41 publications

(32 citation statements)

References 28 publications

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“…WP and HWP samples were diluted 10 to 1000 times and filtered through a 0.2-μm membrane. For monosaccharide analysis, a 25-uL aliquot was injected into a Carbo-Pac PA10 (Dionex, Sunnyvale, CA, USA) column at 1.2 mL/min flow rate as described previously [ 22 ]. The nitrogen in the spent media and biomass was quantified using the Dumas combustion method (AOAC 990.03) (vario Max cube; Elementar Americas Inc., Ronkonkoma, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

Bioconversion of cheese whey permeate into fungal oil by Mucor circinelloides

et al. 2018

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BackgroundOleaginous fungi are efficient tools to convert agricultural waste streams into valuable components. The filamentous fungus Mucor circinelloides was cultivated in whey permeate, a byproduct from cheese production, to produce an oil-rich fungal biomass. Response surface methodology was used to optimize the fermentation conditions such as pH and temperature for increased biomass yield and lipid accumulation. Quantification and characterization of the fungal biomass oil was conducted.ResultsUpstream lactose hydrolysis of the whey permeate increased the biomass yield from 2.4 to 7.8 (g dry biomass/L) compared to that of non-hydrolyzed whey permeate. The combination of low pH (4.5) and pasteurization minimized microbial competition, thus favoring fungal growth. A central composite rotatable design was used to evaluate the effects of temperature (22.4–33.6 °C) and a lower pH range (3.6–4.7) on biomass yield and composition. The highest biomass yield and oil content was observed at high temperature (33.6 °C), while the pH range evaluated had a less pronounced effect. The predictive model was validated at the optimal conditions of 33.6 °C and pH 4.5. The fungal biomass yield plateaued at 9 g dry cell weight per liter, while the oil content and lipid yield reached a maximum of 24% dry biomass and 2.20 g/L, respectively, at 168 h. Triacylglycerides were the major lipid class (92%), which contained predominantly oleic (41%), palmitic (23%), linoleic (11%), and γ-linolenic acid (9%).ConclusionsThis study provided an alternative way of valorization of cheese whey permeate by using it as a substrate for the production of value-added compounds by fungal fermentation. The fatty acid profile indicates the suitability of M. circinelloides oil as a potential feedstock for biofuel production and nutraceutical applications.Electronic supplementary materialThe online version of this article (10.1186/s13036-018-0116-5) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Bioconversion of cheese whey permeate into fungal oil by Mucor circinelloides

et al. 2018

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“…Whey permeate, a low-value by-product obtained after whey proteins have been recovered, contains BMOs and has the potential to serve as a raw material for large-scale oligosaccharide isolation (Dallas et al 2014, Zivkovic & Barile 2011). Membrane filtration can be used to recover oligosaccharides present in whey permeate (Cohen et al 2017, de Moura Bell et al 2016), making these compounds more readily available as a functional food ingredient. Such oligosaccharides recovered from dairy streams could serve as ingredients for infant formula, as their composition is similar to that of HMOs and such an ensemble cannot currently be produced synthetically (Meli et al 2014, Simeoni et al 2016).…”

Section: Bovine Milk Oligosaccharides: Structures and Compositionmentioning

confidence: 99%

“…As mentioned earlier, bovine dairy streams are another promising source for larger-scale isolation of milk oligosaccharides (Barile et al 2009, Cohen et al 2017). A recent clinical study showed that consumption of BMOs by adult volunteers was remarkably well-tolerated, even at dosages of up to 35% of daily fiber intake (Smilowitz et al 2017).…”

Section: Diets Supplemented With Exogenous Milk Oligosaccharidesmentioning

confidence: 99%

Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Kirmiz

Robinson

Shah

et al. 2018

Annu. Rev. Food Sci. Technol.

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106

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Human milk is a unique and complex fluid that provides infant nutrition and delivers an array of bioactive molecules that serve various functions. Glycans, abundant in milk, can be found as free oligosaccharides or as glycoconjugates. Milk glycans are increasingly linked to beneficial outcomes in neonates through protection from pathogens and modulation of the immune system. Indeed, these glycans influence the development of the infant and the infant-gut microbiota. Bifidobacterium species commonly are enriched in breastfed infants and are among a limited group of bacteria that readily consume human milk oligosaccharides (HMOs) and milk glycoconjugates. Given the importance of bifidobacteria in infant health, numerous studies have examined the molecular mechanisms they employ to consume HMOs and milk glycans, thus providing insight into this unique enrichment and shedding light on a range of translational opportunities to benefit at-risk infants.

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“…The prebiotic function of OS is theorized to provide human milk with the key ability to selectively modulate the gut microbiota of infants [ 8 ], and these compounds are believed to make a significant contribution to healthy infant development. The health benefits associated with milk OS have begun to motivate development of large-scale recovery techniques for the dairy industry, which would allow more widespread consumption of the typically underutilized OS in processing streams [ 9 , 10 ]. Growing interest in milk OS, their in vivo functions, and opportunities for their utilization has created a need for efficient detection, characterization and quantification strategies.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags

Robinson

Poulsen

2018

PLoS ONE

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Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent. Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques. Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field. Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards. In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a method for separation of labeled OS on a porous graphitized carbon liquid chromatographic column with subsequent analysis by quadrupole time-of-flight tandem mass spectrometry. Abundances for 15 OS extracted from mature bovine milk were measured, with replicate injections providing coefficients of variation below 15% for most OS. Isobaric labeling improved ionization efficiency for low-abundance, high-molecular weight fucosylated OS, which are known to exist in bovine milk but have been only sporadically reported in the literature. We compared the abundances of four fucosylated OS in milk from Holstein and Jersey cattle and found that three of the compounds were more abundant in Jersey milk, which is in general agreement with a previous study. This novel method represents an advancement in our ability to characterize milk OS and provides the advantages associated with isobaric labeling, including reduced instrumental analysis time and increased analyte ionization efficiency. This improved ability to measure differences in bioactive OS abundances in large datasets will facilitate exploration of OS from all food sources for the purpose of developing health-guiding products for infants, immune-compromised elderly, and the population at large.

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Role of pH in the recovery of bovine milk oligosaccharides from colostrum whey permeate by nanofiltration

Cited by 41 publications

References 28 publications

Bioconversion of cheese whey permeate into fungal oil by Mucor circinelloides

Bioconversion of cheese whey permeate into fungal oil by Mucor circinelloides

Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags

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