Role of phospholipases A2 and C in myocardial ischemic reperfusion injury

Prasad, M. R. N.; Popescu, L. M.; Moraru, Ion I.; Liu, X. K.; Maity, Swapna; Engelman, Richard M.; Das, Dipak K.

doi:10.1152/ajpheart.1991.260.3.h877

Cited by 44 publications

(43 citation statements)

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“…Kuser et al (2000) indicated that lactate is accumulated and glycogen depleted confirming inhibition of aerobic respiration and stimulation of anaerobic glycolysis through hexokinase, a rate limiting enzymes of glycolysis. Some authors have reported that tissue damage followed the release of cellular enzymes such as LDH (Paul et al, 1990;Parasad et al, 1991). Besides the decrease in LDH activity, there was a insignificant change in D-lactate and pyruvate level as compared to untreated snails, as reported by Reddy et al, (1995).…”

Section: Discussionmentioning

confidence: 79%

Protective effects of vitamin C against biochemical toxicity induced by malathion pesticides in male albino rat

Ismail¹

2013

J. Evol. Biol. Res.

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Malathion is a chemical pesticide, commonly used by Egyptian farmers; however, its contamination of water sources has become an important issue. It is suggested that the role of vitamin C in alteration of enzymes responsible for energy metabolism was induced by administration of Malathion. The effect of malathion and malathion with vitamin C on some enzymes in the rat were recorded in the present study. Significant increases in the level of hepatic enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma glutamyl transpeptidase) were recorded. Furthermore, renal markers such as urea, cholesterol and creatinine were increased in rats treated with malathion. Additionally, serum triglyceride was significantly decreased. No significant changes were observed in total protein, albumin and bilirubin in all treated groups as compared to the control. The present results showed that a significant (p < 0.001) decrease in glycolytic enzymes (hexokinase, pyruvatekinase, glucose phosphate isomerase and phosphofructokinase) were observed in treated groups, and lactate dehydrogenase enzyme activity also showed significant (p < 0.01) reduction. Coadministration of vitamin C to the groups restored all the parameters cited above to near-normal values. Therefore, the present investigation revealed that vitamin C appeared to be a promising agent for protection against malathion-induced toxicity.

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Section: Discussionmentioning

confidence: 79%

Protective effects of vitamin C against biochemical toxicity induced by malathion pesticides in male albino rat

Ismail¹

2013

J. Evol. Biol. Res.

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“…Kuser et al [28] indicated that lactate is accumulated and glycogen depleted confirming inhibition of aerobic respiration and stimulation of anaerobic glycolysis through hexokinase, a rate limiting enzymes of glycolysis. Some authors reported that tissue damage followed the release of cellular enzymes such as LDH [29,30]. Besides in spite of the decrease in LDH activity, there was insignificant change in D-lactate and pyruvate level as compared to untreated snails, as reported by Reddy et al [31].…”

Section: Discussionmentioning

confidence: 85%

“…The present results showed that significant reduction in total protein content in treated mice which could be attributed to cellular damage caused by toxins [30]. The main fraction of total protein content is albumin in turn may result from decrease anabolism or increase catabolism [45].…”

Section: Discussionmentioning

confidence: 95%

The role of vitamin C in alteration of enzymes responsible of energy metabolism induced by administration of tamoxifen to mouse

Zuhair¹,

Al-Amri²

2011

ABC

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Tamoxifen is a synthetic non-steroidal ant estrogen. It was suggested to study the role of vitamin C in alteration of enzymes responsible of energy metabolism induced by administration of tamoxifen to mouse. The effect of tamoxifen and tamoxifen with vitamin C on some activity of enzymes in the mice representing glycolytic, gluconeogenic and glycogenolytic pathway and also, liver function enzymes represented by aspartate aminotransferase (AST), alanine amino-transferase (ALT), acid phosphatase (ACP) and alkaline phosphatase (ALP) were studied. The present results showed that a significant (p < 0.001) increase in glycolytic enzymes (HK, PK, GPI and PFK), gluconeogenic enzymes, G-6-Pase, acid phosphatase (ACP), alkaline phosphatase (ALP) and glucose, were observed in treated groups, while LDH, glycogen phosphorylase, AST and ALT enzymes activities showed significant (p < 0.01) reduction. The present results also, showed that significant reduction in glycogen, total protein, total cholesterol, uric acid, urea, and creatinine in treated mice as compared to the normal healthy control group. However, normal control mice treated with tamoxifen and vitamin C showed no side effects of most parameters compared to the normal healthy control group. It was concluded that vitamin C may prevent tamoxifen-induced testes toxicity in mice.

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“…Both could lead to PKC activation [2,22]. Our results with specific anti-PLC and anti-PLA2 antibodies, which bind to and inhibit the cellmembrane enzymes [12,[14][15][16][17], suggest an important cooperative involvement of PLC and PLA2 activity in triggering endocytosis. The endocytosis inhibition induced by PLC and PLA2 blockade is probably due to the failure of subsequent PKC activation because this inhibition is reversed if the assay medium contains the direct PKC activator phorbol 12,13-dibutyrate.…”

Section: Discussionmentioning

confidence: 87%

“…The antibodies dose-dependently inhibit the respective enzymes in living cells [12,[14][15][16][17].…”

Section: Anti-phospholipase Antibodiesmentioning

confidence: 99%

Protein kinase C controls Fcγ receptor‐mediated endocytosis in human neutrophils

Moraru

Laky²,

Stãnescu³

et al. 1990

FEBS Letters

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The aim of this study is to clarify which signaling mechanism operates in Fc7 receptor-mediated endocytosis in human neutrophils. Endocytosis of immune complexes was inhibited by antibodies directed to cell membrane phospholipase C (PLC) and A 2 (PLA2) (maximal inhibition obtained was 57% and 28%, respectively), being almost abolished by these antibodies if used in combination (up to 91~ inhibition). The protein kinase C (PKC) activator, phorbol 12,13-dibutyrate, reversed this inhibitory effect. Four different PKC inhibitors (H-7, palmitoylcarnitine, sphingosine, and tamoxifen) produced a dose-dependent inhibition of endocytosis, up to over 80% in each case. H-8 (I-10/~M) which inhibits cyclic nucleotide protein kinases but not PKC had no effect upon endocytosis. It is concluded that Fc~, receptor-induced activation of PLC and PLA 2 triggers endocytosis by activation of PKC.

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Role of phospholipases A2 and C in myocardial ischemic reperfusion injury

Cited by 44 publications

References 0 publications

Protective effects of vitamin C against biochemical toxicity induced by malathion pesticides in male albino rat

Protective effects of vitamin C against biochemical toxicity induced by malathion pesticides in male albino rat

The role of vitamin C in alteration of enzymes responsible of energy metabolism induced by administration of tamoxifen to mouse

Protein kinase C controls Fcγ receptor‐mediated endocytosis in human neutrophils

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