Role of Phospholipid Asymmetry in the Stability of Inverted Hexagonal Mesoscopic Phases

Mareš, Tomáš; Daniel, Matěj; Perutková, Šárka; Perne, Andrej; Dolinar, Gregor; Iglič, Aleš; Rappolt, Michael; Kralj‐Iglič, Veronika

doi:10.1021/jp805715r

Cited by 33 publications

(35 citation statements)

References 53 publications

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“…This in turn affects the headgroup area to volume ratio which induces a curvature stress causing the transition to non-lamellar phases. In other studies (Mareš et al, 2008; Perutkova et al, 2009; Perutková et al, 2011), a closer look at the pivotal plane radius as a function of chain stiffness and internal curvature revealed that the increase in chain stiffness and internal curvature leads to a reduced pivotal plane radius favoring hexagonal phase transitions.…”

Section: Introductionmentioning

confidence: 72%

Coupling Phase Behavior of Fatty Acid Containing Membranes to Membrane Bio-Mechanics

Tyler

Greenfield

Seddon

et al. 2019

Front. Cell Dev. Biol.

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Biological membranes constantly modulate their fluidity for proper functioning of the cell. Modulation of membrane properties via regulation of fatty acid composition has gained a renewed interest owing to its relevance in endocytosis, endoplasmic reticulum membrane homeostasis, and adaptation mechanisms in the deep sea. Endowed with significant degrees of freedom, the presence of free fatty acids can alter the curvature of membranes which in turn can alter the response of curvature sensing proteins, thus defining adaptive ways to reconfigure membranes. Most significantly, recent experiments demonstrated that polyunsaturated lipids facilitate membrane bending and fission by endocytic proteins – the first step in the biogenesis of synaptic vesicles. Despite the vital roles of fatty acids, a systematic study relating the interactions between fatty acids and membrane and the consequent effect on the bio-mechanics of membranes under the influence of fatty acids has been sparse. Of specific interest is the vast disparity in the properties of cis and trans fatty acids, that only differ in the orientation of the double bond and yet have entirely unique and opposing chemical properties. Here we demonstrate a combined X-ray diffraction and membrane fluctuation analysis method to couple the structural properties to the biophysical properties of fatty acid-laden membranes to address current gaps in our understanding. By systematically doping pure dioleoyl phosphatidylcholine (DOPC) membranes with cis fatty acid and trans fatty acid we demonstrate that the presence of fatty acids doesn’t always fluidize the membrane. Rather, an intricate balance between the curvature, molecular interactions, as well as the amount of specific fatty acid dictates the fluidity of membranes. Lower concentrations are dominated by the nature of interactions between the phospholipid and the fatty acids. Trans fatty acid increases the rigidity while decreasing the area per lipid similar to the properties depicted by the addition of saturated fatty acids to lipidic membranes. Cis fatty acid however displays the accepted view of having a fluidizing effect at small concentrations. At higher concentrations curvature frustration dominates, leading to increased rigidity irrespective of the type of fatty acid. These results are consistent with theoretical predictions as detailed in the manuscript.

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Section: Introductionmentioning

confidence: 72%

Coupling Phase Behavior of Fatty Acid Containing Membranes to Membrane Bio-Mechanics

Tyler

Greenfield

Seddon

et al. 2019

Front. Cell Dev. Biol.

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“…Because the background is consistent with Boltzmann statistics (explicitly independent and indistinguishable constituents), expression (54) can be described as a modified Boltzmann distribution. The explicit independence of constituents in the derivation of the local thermodynamic equilibrium is complemented by introducing the excluded volume effect [the condition (47)] which is reflected in the denominator of Equation (54).…”

Section: Global Equilibrium Of a Multicomponent Membranementioning

confidence: 99%

“…with strongly anisotropically curved structures, such as nanotubular protrusions, 37 tubular and spherical nanovesicles of the erythrocyte membrane, 45 torocyte endovesicles, 46 narrow necks of one-component phospholipid vesicles, 38 two-component vesicles, 34,47 peptidergic vesicles, 48 nanotubules in astrocytes 49 and urothelial cancer cells, 50−52 flattened structures in Golgi bodies, 53 inverse hexagonal lipid phases, 54 and membrane pores. 55,56 While it was previously acknowledged that membrane composition and shape are interdependent, 34,57−59 the orientational ordering model provides a unified explanation of the above feature, and has been reviewed extensively elsewhere.…”

Section: Deviatoric Elasticity May Stabilize Anisotropic Nanostructuresmentioning

confidence: 99%

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Kralj‐Iglič¹

2012

IJN

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Abstract:Membranous nanostructures, such as nanovesicles and nanotubules, are an important pool of biological membranes. Recent results indicate that they constitute cell-cell communication systems and that cancer development is influenced by these systems. Nanovesicles that are pinched off from cancer cells can move within the circulation and interact with distant cells. It has been suggested and indicated by experimental evidence that nanovesicles can induce metastases from the primary tumor in this way. Therefore, it is of importance to understand better the mechanisms of membrane budding and vesiculation. Here, a theoretical description is presented concerning consistently related lateral membrane composition, orientational ordering of membrane constituents, and a stable shape of nanovesicles and nanotubules. It is shown that the character of stable nanostructures reflects the composition of the membrane and the intrinsic shape of its constituents. An extension of the fluid mosaic model of biological membranes is suggested by taking into account curvature-mediated orientational ordering of the membrane constituents on strongly anisotropically curved regions. Based on experimental data for artificial membranes, a possible antimetastatic effect of plasma constituents via mediation of attractive interaction between membranous structures is suggested. This mediated attractive interaction hypothetically suppresses nanovesiculation by causing adhesion of buds to the mother membrane and preventing them from being pinched off from the membrane.

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“…Anisotropic elasticity based theoretical models could explain the emergence of complex membrane geometries like tubules [85, 90–94], sponges and egg-cartons [85, 95, 96]. It was first recognized by Iglic̆ and coworkers [97] that the anisotropic elasticity framework can be used to model the curvature modulating effects of membrane associated proteins.…”

Section: Nematic Membrane Model For Protein Driven Membrane Remodementioning

confidence: 99%

Phenomenology Based Multiscale Models as Tools to Understand Cell Membrane and Organelle Morphologies

Ramakrishnan

Radhakrishnan

2015

Advances in Planar Lipid Bilayers and Liposomes

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An intriguing question in cell biology is “how do cells regulate their shape?” It is commonly believed that the observed cellular morphologies are a result of the complex interaction among the lipid molecules (constituting the cell membrane), and with a number of other macromolecules, such as proteins. It is also believed that the common biophysical processes essential for the functioning of a cell also play an important role in cellular morphogenesis. At the cellular scale—where typical dimensions are in the order of micrometers—the effects arising from the molecular scale can either be modeled as equilibrium or non-equilibrium processes. In this chapter, we discuss the dynamically triangulated Monte Carlo technique to model and simulate membrane morphologies at the cellular scale, which in turn can be used to investigate several questions related to shape regulation in cells. In particular, we focus on two specific problems within the framework of isotropic and anisotropic elasticity theories: namely, (i) the origin of complex, physiologically relevant, membrane shapes due to the interaction of the membrane with curvature remodeling proteins, and (ii) the genesis of steady state cellular shapes due to the action of non-equilibrium forces that are generated by the fission and fusion of transport vesicles and by the binding and unbinding of proteins from the parent membrane.

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Role of Phospholipid Asymmetry in the Stability of Inverted Hexagonal Mesoscopic Phases

Cited by 33 publications

References 53 publications

Coupling Phase Behavior of Fatty Acid Containing Membranes to Membrane Bio-Mechanics

Coupling Phase Behavior of Fatty Acid Containing Membranes to Membrane Bio-Mechanics

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Phenomenology Based Multiscale Models as Tools to Understand Cell Membrane and Organelle Morphologies

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