Role of phospholipid in the activation of Na+, Ka+-activated adenosine triphosphatase of beef brain

Tanaka, Ryo; Strickland, K. P.

doi:10.1016/0003-9861(65)90239-0

Cited by 146 publications

(18 citation statements)

References 16 publications

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“…After homogenization in 0.25M-sucrose containing 5mM-EDTA, pH 7.5, a microsomal fraction was isolated from the rabbit kidney by the procedure described for bovine brain by Tanaka & Strickland (1965): it was resuspended in water and stored frozen.…”

Section: Methodsmentioning

confidence: 99%

“…Treatment with deoxycholate, based on the procedure originally described by Tanaka & Strickland (1965), was not satisfactory because of extreme variability in results, particularly with respect to reactivation of the depleted enzyme (see also Kimelberg & Papahadjopoulos, 1972). After preliminary experiments with the deoxycholate method, therefore, we adopted a modified version of the procedure described by Palatini et al (1972), which uses the non-ionic detergent Lubrol.…”

mentioning

confidence: 99%

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Lipid requirement of the membrane sodium-plus-potassium ion-dependent adenosine triphosphatase system

Wheeler

Walker

Barker

1975

Biochemical Journal

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The dependence of the (Na++K+)-dependent ATPase (adenosine triphosphatase) (EC 3.6.1.3) on lipid has been examined in a number of different ways, with the use of various preparations from kidney tissue. The main findings were as follows. (1) (6) The degree ofreactivation was correlated with the amount ofresidual activity remaining after lipid depletion. (7) Partial purification of the ATPase, giving a 50-fold increase in specific activity, was not accompanied by selective enhancement of any particular class of phospholipid. We conclude that although the ATPase is dependent on phospholipid, only the reactivation results provide evidence for specificity.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Lipid requirement of the membrane sodium-plus-potassium ion-dependent adenosine triphosphatase system

Wheeler

Walker

Barker

1975

Biochemical Journal

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“…This finding is important because it has been shown that proportions of membrane phospholipids, such as PC (Tanaka and Strickland 1965), PS (Tsakiris and Deliconstantinos 1984), and PE (Goldman and Albers 1973) have a significant effect on Na,K-ATPase activity. Therefore, the lower V max of SPM Na,K-ATPase of rats fed low LNA compared with rats fed control or AA + DHA diet is not attributed to altered phospholipid content.…”

Section: Discussionmentioning

confidence: 94%

Dietary low linolenic acid compared with docosahexaenoic acid alter synaptic plasma membrane phospholipid fatty acid composition and sodium–potassium ATPase kinetics in developing rats

Bowen

Clandinin

2002

Journal of Neurochemistry

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The objective of this study was to investigate if maternal dietary 20:4n-6 arachidonic acid (AA) and 22:6n-3 compared with adequate or low levels of 18:3n-3 linolenic acid (LNA) increases synaptic plasma membrane (SPM) cholesterol and phospholipid content, phospholipid 20:4n-6 and 22:6n-3 content, and Na,K-ATPase kinetics in rat pups at two and five weeks of age. At parturition, Sprague-Dawley rats were fed semi-purified diets containing either AA + docosahexaenoic acid (DHA), adequate LNA (control; 18:2n-6 : 18:3n-3 ratio of 7.1 : 1) or low LNA (18:2n-6 : 18:3n-39 ratio of 835 : 1). During the first two weeks of life, the rat pups received only their dams' milk. After weaning, pups received the same diet as their respective dams to five weeks of age. No significant difference was observed among rat pups fed the diet treatments for SPM cholesterol or total and individual phospholipid content at two and five weeks of age. Fatty acid analysis revealed that maternal dietary AA + DHA, compared with feeding the dams the control diet or the low LNA diet, increased 20:4n-6 in phosphatidylserine and 22:6n-3 content of SPM phospholipids. Rats fed dietary AA + DHA or the control diet exhibited a significantly increased V max for SPM Na,K-ATPase. Diet treatment did not alter the K m (affinity) of SPM Na,K-ATPase in rat pups at two and five weeks of age. It is concluded that dietary AA + DHA does not alter SPM cholesterol and phospholipid content but increases the 22:6n-3 content of SPM phospholipids modulating activity of Na,K-ATPase.

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“…ATPase activity was measured by the method of Tanaka and Strickland (7), and hexokinase activity was measured by the method of Darrow and Colowick (8). These enzyme activities were determined in the hy potonic medium according to the original report (4)(5)(6)(7)(8) and the isotonic medium adjusted with sucrose.…”

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confidence: 99%

Influence of Local Administration of Serotonin on Mitochondrial Enzyme Activity of Rat Skeletal Muscle

Arai

Aizawa

1976

Japanese Journal of Pharmacology

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Role of phospholipid in the activation of Na+, Ka+-activated adenosine triphosphatase of beef brain

Cited by 146 publications

References 16 publications

Lipid requirement of the membrane sodium-plus-potassium ion-dependent adenosine triphosphatase system

Lipid requirement of the membrane sodium-plus-potassium ion-dependent adenosine triphosphatase system

Dietary low linolenic acid compared with docosahexaenoic acid alter synaptic plasma membrane phospholipid fatty acid composition and sodium–potassium ATPase kinetics in developing rats

Influence of Local Administration of Serotonin on Mitochondrial Enzyme Activity of Rat Skeletal Muscle

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