Role of Platelet-Derived Growth Factors in the Testis

Basciani, Sabrina; Mariani, Stefania; Spera, Giovanni; Gnessi, Lucio

doi:10.1210/er.2010-0004

Cited by 75 publications

(54 citation statements)

References 175 publications

(248 reference statements)

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“…Previous studies indicated that DHH and PDGF are involved in the normal development of the fetal and adult Leydig cell populations (25,26,(29)(30)(31)(32). The specific roles of these factors in the functions of stem Leydig cells in adult testes were not elucidated.…”

Section: Lh -+ ----+ ----+ ---Dbcamp--+ ----+ ----+ --22hc ---+ ----+mentioning

confidence: 99%

Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes

Wang

Jiang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

126

197

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Testicular Leydig cells are the primary source of testosterone in males. Adult Leydig cells have been shown to arise from stem cells present in the neonatal testis. Once established, adult Leydig cells turn over only slowly during adult life, but when these cells are eliminated experimentally from the adult testis, new Leydig cells rapidly reappear. As in the neonatal testis, stem cells in the adult testis are presumed to be the source of the new Leydig cells. As yet, the mechanisms involved in regulating the proliferation and differentiation of these stem cells remain unknown. We developed a unique in vitro system of cultured seminiferous tubules to assess the ability of factors from the seminiferous tubules to regulate the proliferation of the tubule-associated stem cells, and their subsequent entry into the Leydig cell lineage. The proliferation of the stem Leydig cells was stimulated by paracrine factors including Desert hedgehog (DHH), basic fibroblast growth factor (FGF2), platelet-derived growth factor (PDGF), and activin. Suppression of proliferation occurred with transforming growth factor β (TGF-β). The differentiation of the stem cells was regulated positively by DHH, lithium- induced signaling, and activin, and negatively by TGF-β, PDGFBB, and FGF2. DHH functioned as a commitment factor, inducing the transition of stem cells to the progenitor stage and thus into the Leydig cell lineage. Additionally, CD90 (Thy1) was found to be a unique stem cell surface marker that was used to obtain purified stem cells by flow cytometry.

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Section: Lh -+ ----+ ----+ ---Dbcamp--+ ----+ ----+ --22hc ---+ ----+mentioning

confidence: 99%

Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes

Wang

Jiang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

126

197

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“…Transcripts of rat Pdgfrb are detected at embryonic day 18 (E18), reach high levels on 5 dpn, and then decline to lower levels [72]. Pdgfr can activate gonocytes to migrate from the central region of the seminiferous tubule to the basal lamina of the seminiferous epithelium and initiate their differentiation to spermatogonial stem cells [73], which suggests that gene transcription of the MAPK pathway in the spermatogenic cells should be more active in PN1. Moreover, Sertoli cells in PN2 are nearly mature, which makes the MAPK pathway activated by FSH repressive.…”

Section: Differential Expression Of Genes In Cellular Signaling Pathwaysmentioning

confidence: 99%

Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

Gong

Pan

Lin

et al. 2012

Sci. China Life Sci.

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Mammalian testis development is a complex and highly sophisticated process. To study the dynamic change of normal testis development at the transcriptional level, we investigated mouse testes at three postnatal ages: 6 days postnatal, 4 weeks old, and 10 weeks old, representing infant (PN1), juvenile (PN2), and adult (PN3) stages, respectively. Using ultra high-throughput RNA sequencing (RNA-seq) technology, we obtained 211 million reads with a length of 35 bp. We identified 18837 genes that were expressed in mouse testes, and found that genes expressed at the highest level were involved in spermatogenesis. The gene expression pattern in PN1 was distinct from that in PN2 and PN3, which indicates that spermatogenesis has commenced in PN2. We analyzed a large number of genes related to spermatogenesis and somatic development of the testis, which play important roles at different developmental stages. We also found that the MAPK, Hedgehog, and Wnt signaling pathways were significantly involved at different developmental stages. These findings further our understanding of the molecular mechanisms that regulate testis development. Our study also demonstrates significant advantages of RNA-seq technology for studying transcriptome during development. next-generation sequencing, transcriptome, mouse testis, development Citation:Gong W, Pan L L, Lin Q, et al. Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing.

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“…We analyzed differentially expressed genes related to male reproductive function using GO analysis. In total, eight differentially expressed genes were found in newborn mice of the ICSI group, among which Amh and Rhox5 are expressed in Sertoli cells (Hu et al, 2007;Goulis et al, 2008), Adcy1p1r1 and Pdgfra are expressed in Leydig cells (Koh and Won, 2006;Basciani et al, 2010), while the other four genes are mainly expressed in spermatocytes (Lee et al, 2002;Abreu et al, 2010;Bellani et al, 2010;Puverel et al, 2011). There are two essential stages for testicle development: embryonic and pubertal age.…”

Section: Discussionmentioning

confidence: 99%

“…PDGFRA is essential for early embryonic development of Leydig cells (Basciani et al, 2010). Testosterone is produced by the Leydig cells.…”

Section: Discussionmentioning

confidence: 99%

Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice

Wang

Fang

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:Intracytoplasmic sperm injection (ICSI) is commonly used to solve male infertility problems. Previous studies showed that early environmental exposure of an embryo may influence postnatal development. To detect whether ICSI operations affect the reproductive health of a male or his offspring, we established assisted reproductive technologies (ART) conceived mouse models, and analyzed gene expression profiles in the testes of both ICSI and naturally conceived (NC) newborn F 1 mice using micro-array analysis. Among the differentially expressed genes, we focused on the expression of eight male reproduction-related genes. Quantitative real-time reverse transcriptionpolymerase chain reaction (qRT-PCR) was used to analyze the expression of these genes in the testes of both adult and old F 1 generation mice and adult F 2 generation mice. Our results showed that down-regulated and somatic cell-expressed genes in newborn mice retained their differential expression patterns in adult and old F 1 generation individuals, implying the persistence and fetal origin of the alteration in the expression of these genes. The intergenerational transmission of differential gene expression was observed, but most changes tended to be reduced in adult F 2 generations. Controlled ovarian hyperstimulation (COH) and in vitro fertilization (IVF) mice models were added to explore the precise factors contributing to the differences in ICSI offspring. The data demonstrated that superovulation, in vitro culture, and mechanical stimulation involved in ICSI had a cumulative effect on the differential expression of these male reproductive genes.

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Role of Platelet-Derived Growth Factors in the Testis

Cited by 75 publications

References 175 publications

Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes

Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes

Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice

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