Role of platelet-derived microparticles in transfer of the chemokine receptor CXCR4 to CXCR4-negative cells

Manoochehrabadi, Tahereh; Sharifi, Z; Yari, Fatemeh

doi:10.47176/mjiri.33.55

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…Experimental studies have shown that CXCR4 can promote tumor progression through direct and indirect mechanisms. It mainly involves the following pathways: first, CXCR4 can induce tumor cells to metastasize to CXCL12positive tissues and organs [29,30] and thus cause the entry of tumor cells into the bone marrow cell, which allows the tumor cells to escape from the body's immune killing effect, which is beneficial to tumor survival and growth. Secondly, the interstitial cell-derived CXCL12 itself stimulates the survival and growth of neoplastic cells in a paracrine manner.…”

Section: Discussionmentioning

confidence: 99%

The Value and Clinical Significance of Tumor Marker Detection in Cervical Cancer

Sun

Shen

Yuan

et al. 2021

Scientific Programming

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When it comes to cervical cancer, it is the most common malignancy in gynecology. This study aimed to investigate the concomitant status of miRNA-9-5p in cervical cancer and explore its potential mechanism for treating cervical cancer. The levels of miRNA-9-5p, CA125, CA199, and CEA expression were detected by RT-PCR, and the downstream target genes regulated by miRNA-9-5p were screened by the Venn map. Cytoscape was utilized to find the binding sites of the two genes, and luciferase reporter assay verified the direct regulation of miRNA-9-5p and CXCR4; the CCK-8 assay detected its regulation on cell proliferation, and the expression of miRNA-9-5p, CXCR4, PCNA, Ki67 mRNA, and proteins was detected by RT-PCR and western blot. The expression of miRNA-9-5p was decreased, while the levels of CA125, CA199, and CEA were increased in the model group. The database predicts that CXCR4 is a gene regulated by miRNA-9-5p. The luciferase reporter gene results indicated that miRNA-9-5p could directly regulate the expression of CXCR4 and miRNAs are detected by intracellular transfer inhibitors. In total, MiRNA-9-5p can be utilized as a biological marker for cervical cancer that may inhibit cancer cells’ proliferation by inhibiting the expression of the CXCR4 gene and protein.

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Section: Discussionmentioning

confidence: 99%

The Value and Clinical Significance of Tumor Marker Detection in Cervical Cancer

Sun

Shen

Yuan

et al. 2021

Scientific Programming

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“…PEVs could also transfer CXCR4 to unexpressed tumor cells, confirmed in various solid tumor cell lines. 67 CXCR4 is a chemokine receptor, stimulating tumor cell growth and migration through various downstream pathways. 68,69 On the other hand, however, Michael and colleagues demonstrated that miRNAs transferred by PEVs could induce the apoptosis of Lewis lung carcinoma cells and suppress tumor growth in vivo.…”

Section: Biology and Function Of Pevsmentioning

confidence: 99%

Platelet-derived extracellular vesicles for drug delivery

Yao¹,

Wang²

2023

Biomater. Sci.

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“…The standard curve was drawn against BSA samples and was used for determination of the MPs concentrations. 23…”

Section: Determination Of Pmps Concentration Based On Bradford Assaymentioning

confidence: 99%

“…After 20 minutes incubation at room temperature and in darkness, the fluorescence activated cell sorter analysis was performed using FACSCalibur. 23…”

Section: Phenotypic Characterization Of Pmps By Flow Cytometrymentioning

confidence: 99%

Platelet Microparticles Accelerate Proliferation and Growth of Mesenchymal Stem Cells through Longevity-Related Genes

et al. 2021

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Background: Due to their self-renewal and differentiation ability, the mesenchymal stem cells (MSCs) have been studied extensively. However, the MSCs lifespan is restricted; they undergo several divisions in vitro that cause several alternations in cellular features and relatively lessens their application. Thus, this study was aimed to assess the effect of platelet-derived microparticles (PMPs), a valuable source of proteins, microRNAs (miRNAs), and growth factors, on the expression of hTERT, c-MYC, p16, p53, and p21 as the most important aging and cell longevity genes alongside with population doubling time (PDT) of PMP-treated cells in comparison to a control group. Methods: Umbilical cord MSCs (UC-MSCs) were used in this study, whereby they reached a confluency of 30%. MSCs were treated by PMPs (50 µg/mL), and then, PDT was determined for both groups. Quantitative expression of hTERT, c-MYC, p16, p53, and p21 was examined through quantitative real-time PCR at various intervals (i.e. after five and thirty days as well as freezing-thawing process). Results: Our results demonstrated that the treated group had a shorter PDT in comparison to the control group (P<0.050). The real-Time PCR data also indicated that PMPs were able to remarkably up-regulate hTERT and c-MYC genes expression while down-regulating the expression of p16, p21, and p53 genes (P<0.050), especially following five days of treatment. Conclusion: According to these data, it appears that PMPs are a safe and effective candidate for prolonging the lifespan of UC-MSCs; however, further investigations are needed to corroborate this finding.

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Role of platelet-derived microparticles in transfer of the chemokine receptor CXCR4 to CXCR4-negative cells

Abstract: What is "already known" in this topic: PMPs transfer CXCR4 to null cells (Daudi, K562 and U937 cell lines).

Cited by 3 publications

References 19 publications

The Value and Clinical Significance of Tumor Marker Detection in Cervical Cancer

The Value and Clinical Significance of Tumor Marker Detection in Cervical Cancer

Platelet-derived extracellular vesicles for drug delivery

Platelet Microparticles Accelerate Proliferation and Growth of Mesenchymal Stem Cells through Longevity-Related Genes

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