Role of Polymorphonuclear Neutrophils in a Murine Model of<i>Chlamydia psittaci</i>-Induced Abortion

Buendía, A. J.; de, Roberto Montes; Navarro, J. A.; Sánchez, J.; Cuello, F.; Salinas, J.

doi:10.1128/iai.67.5.2110-2116.1999

Cited by 47 publications

(10 citation statements)

References 42 publications

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“…Investigation of this dendritic cell population in the spleen revealed no difference in cell number between mice given mAb RB6–8C5, rat IgG, and PBS. Our study used a single and relatively small dose of mAb (25 μg/mouse) which was 4–20‐times smaller than the doses used in comparable studies 3, 6, 11–13, 43, 44. Hence, the most straightforward interpretation of the data is that theeffects observed were due to neutrophil depletion.…”

Section: Discussionmentioning

confidence: 93%

Neutropenia alters lung cytokine production in mice and reduces their susceptibility to pulmonary cryptococcosis

et al. 2003

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Neutrophils are generally considered to contribute to host defense through their potent microbicidal activity. However, there is accumulating evidence that neutrophils also have an important regulatory role in establishing the balance of Th1 and Th2 responses. This study investigated the role of neutrophils in defense against pulmonary Cryptococcus neoformans infection using neutrophil-depleted BALB/c mice generated by administering mAb RB6-8C5. Neutropenic mice with pulmonary infection survived significantly longer than control mice, but there was no difference between groups infected intravenously. On day 1 of infection, neutropenic mice had significantly smaller fungal burdens than control mice. On day 7, neutropenic mice had significantly higher lung concentrations of IL-10, TNF- § , IL-4, and IL-12 than control mice, but there was no difference in IFN-+ and MCP-1 levels. Neutrophils influenced the outcome of cryptococcal infection in mice through mechanisms that did not involve a reduction in early fungal burden. The absence of neutrophils in lung tissue during the initial stages of infection appeared to alter the inflammatory response in a manner that was subsequently beneficial to the host. Higher levels of Th1-and Th2-associated cytokines in neutropenic mice could have simultaneously promoted a strong cellular response while reducing inflammatory damage to the lung. Our results support the emerging concept that neutrophils play an important function in modulating the development of the immune response.

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Section: Discussionmentioning

confidence: 93%

Neutropenia alters lung cytokine production in mice and reduces their susceptibility to pulmonary cryptococcosis

et al. 2003

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“…Aluminum salts promotes formation of multimolecular aggregates, when it interacts with the antigen and these aggregates or multimolecular structure are more easily phagocytized ( 46 ). Moreover, DEAE-Dextran is commonly used to facilitate the infection of cell cultures in vitro ( 33 ) as it is a molecule that enhances the adhesion of Chlamydiae to target cells, which may increase the amount of antigen reaching the antigen presenting cells located in the immunization zone ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

“…In mice experimental design, the evaluation of the infection was performed by counting the IFUs of the liver and spleen isolated on McCoy cell monolayers, based on the method previously described by Buendia et al ( 33 ). The number of inclusions in McCoy cells was counted under fluorescent microscopy to calculate the number of IFUs per gram of tissue.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Efficacy of a New Commercially Available Inactivated Vaccine Against Ovine Enzootic Abortion

Montbrau¹,

Fontseca²,

March³

et al. 2020

Front. Vet. Sci.

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Ovine enzootic abortion (OEA), caused by Chlamydia abortus , is an economically important disease in many countries. Inactivated vaccines have been used for many years as they induce immunity in sheep, although outbreaks of abortions have been described in vaccinated flocks. In addition, there is a commercially available live attenuated vaccine that provides good protective results. Recently however, reports question the attenuation of this vaccine and associate it with the appearance of outbreaks of OEA in vaccinated flocks. In the present study, a recently commercialized inactivated vaccine (INMEVA®; Laboratorios Hipra S.A., Amer, Spain) has been evaluated using mouse and sheep experimental models. In the mouse models (non-pregnant and pregnant models), the efficacy of INMEVA vaccine has been compared to an unvaccinated control group and to an experimental inactivated vaccine considered as a positive protection control (UMU vaccine). In the non- pregnant model, the UMU vaccine was more effective than the INMEVA vaccine regarding the impact on body weight or the presence of C. abortus in the liver, but both vaccinated groups (UMU and INMEVA) had significantly lower C. abortus in the liver compared to the control group. In the pregnant model in terms of reproductive failures, pups per mouse or the presence of C. abortus in the liver or uterus, no significant differences were found between both vaccines, inducing protection compared to the control group. In the ovine pregnant model, where INMEVA vaccine was compared only to an unvaccinated group, the results indicate that this new commercial vaccine is safe and provides a suitable level of protection against an experimental challenge with C. abortus . A 75% reduction in reproductive disorders, 55% reduction in animals with C. abortus shedding on day of parturition/abortion, and a significant reduction of the average amount of chlamydial shedding from parturition/abortion over the next 21 days was observed, in relation to the infected control group. The results suggest that this vaccine is adequate for the control and prevention of OEA; however, future studies are necessary to elucidate the type of protective immune response that it induces.

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“…). These neutrophils produce reactive oxygen species as well as enzymes to eradicate chlamydiae and represent the first line of defense against bacterial infections in functional cooperation with dendritic cells (DCs) and macrophages (Fig. ).…”

Section: Cell‐autonomous Innate and Adaptive Immune Responses Againmentioning

confidence: 99%

Chlamydia–host cell interaction not only from a bird's eye view: some lessons from Chlamydia psittaci

et al. 2016

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Edited by Wilhelm JustChlamydia psittaci causes psittacosis/ornithosis in birds and is an economically important pathogen for poultry farming. It also infects nonavian domestic animals as well as rodents, and is a zoonotic human pathogen responsible for atypical pneumonia. The bacterium efficiently disseminates in host organisms causing pulmonary and systemic disease. Its rapid entry, fast replication cycle, and tight control of intracellular transport routes contribute to the host-to-host transmission and efficient growth observed with C. psittaci. Recent studies have revealed that the pathogen copes better than other chlamydial strains with proinflammatory effectors produced during the early immune reaction of infected hosts. These features likely contribute to successful infections and might explain the potent adaptation and evasion characteristics of the agent. Current findings on cell-autonomous, innate, and adaptive defenses against C. psittaci provide novel insights into the concerted immune mechanisms involved in the clearance of the pathogen. Further in-depth studies on C. psittaci and other related agents in cellular as well as animal models are needed to develop more efficient antichlamydial therapies and vaccination strategies.

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Role of Polymorphonuclear Neutrophils in a Murine Model ofChlamydia psittaci-Induced Abortion

Cited by 47 publications

References 42 publications

Neutropenia alters lung cytokine production in mice and reduces their susceptibility to pulmonary cryptococcosis

Neutropenia alters lung cytokine production in mice and reduces their susceptibility to pulmonary cryptococcosis

Evaluation of the Efficacy of a New Commercially Available Inactivated Vaccine Against Ovine Enzootic Abortion

Chlamydia–host cell interaction not only from a bird's eye view: some lessons from Chlamydia psittaci

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