Role of Potyviruses in Synergistic Interaction Leading to Maize Lethal Necrotic Disease on Maize

Mbega, Ernest R.; Ndakidemi, P. A.; Mamiro, D. P.; Mushongi, Arnold; Kitenge, K. M.; Ndomba, O. A.

doi:10.20546/ijcmas.2016.506.011

Cited by 9 publications

(25 citation statements)

References 47 publications

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“…Further, the study detected high heritability estimates (0.71 to 0.94) for resistance to MLN in all the seven populations. Previous investigations revealed moderate to high heritability estimates (0.34-0.89) for resistance to MLN [7,22,48,51]. The high heritability estimates detected in this study corroborate earlier reports and strongly suggest that resistance to MLN could be largely influenced by several genes with major effects.…”

Section: Response Of Parents and F3 Populations To Mln Infectionssupporting

confidence: 90%

Genetic Analysis of QTL for Resistance to Maize Lethal Necrosis in Multiple Mapping Populations

Awata

Beyene

Gowda

et al. 2019

Genes

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Maize lethal necrosis (MLN) occurs when maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) co-infect maize plant. Yield loss of up to 100% can be experienced under severe infections. Identification and validation of genomic regions and their flanking markers can facilitate marker assisted breeding for resistance to MLN. To understand the status of previously identified quantitative trait loci (QTL)in diverse genetic background, F3 progenies derived from seven bi-parental populations were genotyped using 500 selected kompetitive allele specific PCR (KASP) SNPs. The F3 progenies were evaluated under artificial MLN inoculation for three seasons. Phenotypic analyses revealed significant variability (P ≤ 0.01) among genotypes for responses to MLN infections, with high heritability estimates (0.62 to 0.82) for MLN disease severity and AUDPC values. Linkage mapping and joint linkage association mapping revealed at least seven major QTL (qMLN3_130 and qMLN3_142, qMLN5_190 and qMLN5_202, qMLN6_85 and qMLN6_157 qMLN8_10 and qMLN9_142) spread across the 7-biparetal populations, for resistance to MLN infections and were consistent with those reported previously. The seven QTL appeared to be stable across genetic backgrounds and across environments. Therefore, these QTL could be useful for marker assisted breeding for resistance to MLN.

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Section: Response Of Parents and F3 Populations To Mln Infectionssupporting

confidence: 90%

Genetic Analysis of QTL for Resistance to Maize Lethal Necrosis in Multiple Mapping Populations

Awata

Beyene

Gowda

et al. 2019

Genes

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“…(MCMV) and a potyvirus, in recent outbreaks this is usually Sugarcane mosaic virus (SCMV). Other members of the potyviridae, including Maize dwarf mosaic virus 10 or Wheat streak mosaic virus 6 , have also been associated with the disease. It is thought that the second virus supresses the host plants post-transcriptional gene silencing based antiviral defences allowing for increased replication of MCMV and subsequent devastating disease 6 .…”

Section: Maize Lethal Necrosis (Mln) Is Caused By a Co-infection Betwmentioning

confidence: 99%

“…Other members of the potyviridae, including Maize dwarf mosaic virus 10 or Wheat streak mosaic virus 6 , have also been associated with the disease. It is thought that the second virus supresses the host plants post-transcriptional gene silencing based antiviral defences allowing for increased replication of MCMV and subsequent devastating disease 6 . Field surveys using ELISA in Kenya in showed that 60 % of randomly selected samples were positive for MCMV, whilst only 28% were infected with SCMV 8 .…”

Section: Maize Lethal Necrosis (Mln) Is Caused By a Co-infection Betwmentioning

confidence: 99%

Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa

Adams

Braidwood

Stomeo³

et al. 2017

Preprint

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Maize lethal necrosis disease (MLN) is an emerging disease in East Africa caused by the introduction of Maize chlorotic mottle virus (MCMV). Recent activity seeking to limit spread of the disease is reliant on effective diagnostics. Traditional diagnostics applied on samples with typical field symptoms of MLN have often given negative results using ELISA or PCR for MCMV and Sugarcane mosaic virus (SCMV). Samples collected in the field with typical MLN symptoms were examined using next generation sequencing (NGS). SCMV was found to be more prevalent than suggested by targeted diagnostics. Additionally, the panel of samples were found to be infected with a range of other viruses, seven of which are described here for the first time. Although not previously identified in the region, Maize yellow mosaic virus (MYMV) was the most prevalent virus after MCMV. The development of targeted diagnostics for emerging viruses is complicated when the extent of field variation is unknown, something that can be negated by using NGS methods. As a result we explored MinION technology which may be more readily deployable in resource poor settings. The results show that this sequencer can diagnose known viruses and future iterations have the potential to identify novel viruses.

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“…Life cycle of viruses is determined by their replication processes inside the host. Unlike DNA viruses, where their reproduction processes begin inside the host nucleus, multiplication processes of the MLN viruses occur in the cytoplasm of the host cells (Ryabov et al, 1999;Mbega et al, 2016;Ivanov et al, 2014). Viral particle enters host cell through wounds made mechanically or by vectors on the cell wall or by deposition into an ovule by an infected pollen grain (Stenger and French, 2008;Zhang-ying et al, 2008;Xie et al, 2011).…”

Section: Infectious Cycle Of Mln Virusesmentioning

confidence: 99%

Maize lethal necrosis and the molecular basis of variability in concentrations of the causal viruses in co-infected maize plant

Awata¹,

Ifie

Tongoona

et al. 2019

J. Gen. Mol. Virol.

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Role of Potyviruses in Synergistic Interaction Leading to Maize Lethal Necrotic Disease on Maize

Cited by 9 publications

References 47 publications

Genetic Analysis of QTL for Resistance to Maize Lethal Necrosis in Multiple Mapping Populations

Genetic Analysis of QTL for Resistance to Maize Lethal Necrosis in Multiple Mapping Populations

Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa

Maize lethal necrosis and the molecular basis of variability in concentrations of the causal viruses in co-infected maize plant

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