Role of progesterone concentrations during early follicular development in beef cattle: I. Characteristics of LH secretion and oocyte quality

Abreu, F. M.; Silva, M.A. Coutinho da; Cruppe, L. H.; Mussard, M.L.; Bridges, G.A.; Harstine, B.R.; Smith, George W.; Geary, T. W.; Day, M. L.

doi:10.1016/j.anireprosci.2018.06.020

Cited by 31 publications

(18 citation statements)

References 49 publications

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“…Previous reports showed relatively lesser progesterone (P4) concentrations could increase follicle diameter in Bos indicus and Bos taurus . At the same time lesser P4 concentrations during early follicular development can accelerate follicular growth . Steroid hormone synthesis of GCs is regulated by many factors, including LH, FSH, PRL, and melatonin …”

Section: Introductionmentioning

confidence: 99%

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Liu

Yang

et al. 2019

Journal of Pineal Research

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The interaction of granulosa cells (GCs) with oocytes is important to regulate follicle development. The exogenous melatonin promoting the maturation of oocytes by GCs has been approved in pig, however, the transcriptome profile and the functions of the genes regulated by melatonin in GCs have not yet to be fully characterized. In this study, we found melatonin could stimulate the synthesis of estradiol in pig GCs. The RNA‐seq was used to explore the effects of melatonin on gene expression, a total of 89 differentially expressed genes (DEGs) were identified. Gene ontology analysis showed DEGs which associated with regulation of cell proliferation, cell cycle, and anti‐apoptosis were significantly enriched. The functions of two DEGs, NOTCH2 and FILIP1L, were studied in pig GCs. The results showed that NOTCH2 inhibited the synthesis of estradiol, but FILIP1L promoted the synthesis of estradiol. Furthermore, inhibiting NOTCH2 in granulosa cells cocultured with cumulus‐oocyte‐complexes had no obvious effect on the maturation of pig oocyte, but could upregulate the cleavage rate of oocyte. We proved that FILIP1L had no effect on the maturation and cleavage of pig oocytes. Our work deepens the understanding of melatonin's effects on GCs and oocyte. The DEGs we found will be beneficial to reveal mechanisms of melatonin acting on GCs and oocytes and design the pharmacological interventions.

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Section: Introductionmentioning

confidence: 99%

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Liu

Yang

et al. 2019

Journal of Pineal Research

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“…The concentration of LH in the current experiment did not show significantly different between treatment (Table 3) due to a high variation in the data that was probably caused by different individual responses, although the concentration of LH tended to be higher in the C group in comparison with A, B, and D groups (Figure 2). The con-centration of LH was lower compared to basal LH, about 1.17-1.58 ng/ml (Abreu et al, 2018), when the increase of LH concentration was higher than the basal level, the cows then experienced ovulation (Figure 2). Thus, bolus containing herbal (grounded Moringa (Rahman) and vitamin A, D, E as well as Zinc picolinate supplementation were enough to maintain the concentration of LH in response to ovulation and the growth of corpus luteum, indicating a normal reproduction cycle (Thatcher, 2017).…”

Section: Results and Discussion The Concentration Of Fsh And Lhmentioning

confidence: 98%

Improving the Reproductive Performance of Cows Suffering from Ovarian Hypofunction using Herbal Supplement

Affandhy¹,

Luthfi²,

Firdaus³

et al. 2021

AAVS

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This study aimed to solve the reproductive problems in crossbred cows suffering from ovarian hypofunction (HPO). Twenty-four cows were divided into four treatments with six replications, treatment A concentrate feed (control); treatment B: control + premix; treatment C control + two boluses, and treatment D control + four boluses. The experimental period was 42 days (two estrous cycles). The bolus formulation consisted of vitamin A, vitamin D, vitamin E, zinc, Moringa oleifera and alginate powder. Data analysis using oneway ANOVA with a Completely Randomized Design. The results showed that the lowest number of follicular palpations in cows suffered from HPO was 67% compared to 83% premix administration, while all cows given bolus were obtained follicular palpation or cured 100%. The results showed that the follicle diameters of the four treatments were not significant difference, but the results of rectal palpation were almost palpable, except treatment A on day 21 that could not be palpated on the left follicle and no corpus luteum was found, whereas in treatments B, C and D partially luteal phase were found. It was concluded that the best treatment of HPO in cows suffered from HPO was by providing two boluses, and was administered twice a week.

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“…from day 10 to day 12 or from day 7 to day 9) were lower in the G‐12 SOV ewes compared with G‐9 SOV animals; we did not measure serum P 4 concentrations in the ewes of the present study. In cattle, lower P 4 concentrations in late metestrus and proestrus are associated with a greater luteinizing hormone (LH) pulse frequency (Abreu et al., 2018), and LH upregulates the expression of its receptors and various proteins linked to proliferation and functionality of ovarian follicular cells (Luo et al., 2011). The same mechanism may be responsible for lower follicular oestrogenicity and delayed onset of oestrus in G‐9 SOV ewes.…”

Section: Discussionmentioning

confidence: 99%

“…Duration of cervical penetration with mandrel/catheter (min) pulse frequency (Abreu et al, 2018), and LH upregulates the expression of its receptors and various proteins linked to proliferation and functionality of ovarian follicular cells (Luo et al, 2011). The same mechanism may be responsible for lower follicular oestrogenicity and delayed onset of oestrus in G-9 SOV ewes.…”

Section: Discussionmentioning

confidence: 99%

A study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non‐surgical uterine flushing

Oliveira

Arrais

Mello

et al. 2022

Reprod Domestic Animals

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The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9‐day (G‐9SOV, n = 21) or 12‐day (G‐12SOV, n = 21) progesterone (P4)‐based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle‐stimulating hormone (133 mg of pFSH given over 3 days). Non‐surgical embryo recovery (NSER) was performed 6–7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G‐9SOV and G‐12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G‐12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G‐9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G‐12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G‐9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ≥2 mm in diameter) at the sixth pFSH dose (at P4‐device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G‐12SOV. The ewes with ≥10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G‐12SOV group had better superovulatory responses compared with G‐9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ≥10 ovulations are main contributors to viable embryo production in Morada Nova ewes.

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Role of progesterone concentrations during early follicular development in beef cattle: I. Characteristics of LH secretion and oocyte quality

Cited by 31 publications

References 49 publications

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Improving the Reproductive Performance of Cows Suffering from Ovarian Hypofunction using Herbal Supplement

A study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non‐surgical uterine flushing

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