Role of proneural genes in the formation of the larval olfactory organ of Drosophila

Grillenzoni, Nicola; Vaux, Véronique de; Meuwly, Jocelyne; Vuichard, Séverine; Jarman, Andrew P.; Holohan, Eimear E.; Gendre, Nanaë; Stocker, Reinhard F.

doi:10.1007/s00427-007-0135-6

Cited by 16 publications

(23 citation statements)

References 50 publications

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“…Although the neuronal composition of the larval chemosensory system is well established in Drosophila (Grillenzoni et al 2007;Stocker 2008), much less is known about the accessory structures. Studies in Musca domestica show that the DO is filled by a large fluid-containing vacuole from the proximal region where the cellular bodies lie to the distal lumen of the dome.…”

Section: Atk Localizes To the Supporting Ecm Of Type I Sensory Organsmentioning

confidence: 99%

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

et al. 2014

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Sensory cilia are often encapsulated by an extracellular matrix (ECM). In Caenorhabditis elegans, Drosophila melanogaster, and vertebrates, this ECM is thought to be directly involved in ciliary mechanosensing by coupling external forces to the ciliary membrane. Drosophila mechano-and chemosensory cilia are both associated with an ECM, indicating that the ECM may have additional roles that go beyond mechanosensory cilium function. Here, we identify Artichoke (ATK), an evolutionarily conserved leucine-rich repeat ECM protein that is required for normal morphogenesis and function of ciliated sensilla in Drosophila. atk is transiently expressed in accessory cells in all ciliated sensory organs during their late embryonic development. Antibody stainings show ATK protein in the ECM that surrounds sensory cilia. Loss of ATK protein in atk null mutants leads to cilium deformation and disorientation in chordotonal organs, apparently without uncoupling the cilia from the ECM, and consequently to locomotion defects. Moreover, impaired chemotaxis in atk mutant larvae suggests that, based on ATK protein localization, the ECM is also crucial for the correct assembly of chemosensory receptors. In addition to defining a novel ECM component, our findings show the importance of ECM integrity for the proper morphogenesis of ciliated organs in different sensory modalities.

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Section: Atk Localizes To the Supporting Ecm Of Type I Sensory Organsmentioning

confidence: 99%

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

et al. 2014

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“…Using immunohistochemical staining and confocal microscopy, we have characterized dll expression during the development of the larval chemosensory system. The larval chemosensory system is specified and differentiates during embryogenesis and is fully formed by hatching (13,25,26). dll is expressed in the developing larval chemosensory organs throughout embryogenesis ( Fig.…”

Section: Dll Is Expressed During the Development Of Peripheral And Cementioning

confidence: 99%

“…Fourteen sense organ precursors (SOPs) contribute to the DO (13). Seven of these give rise to the olfactory portion of the DO, and seven are gustatory.…”

mentioning

confidence: 99%

“…Relatively few genes have been identified to date that function during DO development. These include three basic helix-loop-helix (bHLH) transcription factor-encoding proneural genes: atonal (ato), absent multidendritic neurons and olfactory sensilla (amos), and scute (sc) (13). Specification of four of the olfactory SOPs requires ato while three require amos, and the DO gustatory SOPs are specified by sc (13).…”

mentioning

confidence: 99%

“…These include three basic helix-loop-helix (bHLH) transcription factor-encoding proneural genes: atonal (ato), absent multidendritic neurons and olfactory sensilla (amos), and scute (sc) (13). Specification of four of the olfactory SOPs requires ato while three require amos, and the DO gustatory SOPs are specified by sc (13). The cephalic gap genes orthodenticle (otd), empty spiracles (ems), and buttonhead (btd), which are coexpressed in the antennal head segment, also are required for DO development.…”

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confidence: 99%

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Homeobox gene distal-less is required for neuronal differentiation and neurite outgrowth in the Drosophila olfactory system

Plavicki¹,

Mader²,

Pueschel

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Vertebrate Dlx genes have been implicated in the differentiation of multiple neuronal subtypes, including cortical GABAergic interneurons, and mutations in Dlx genes have been linked to clinical conditions such as epilepsy and autism. Here we show that the single Drosophila Dlx homolog, distal-less , is required both to specify chemosensory neurons and to regulate the morphologies of their axons and dendrites. We establish that distal-less is necessary for development of the mushroom body, a brain region that processes olfactory information. These are important examples of distal-less function in an invertebrate nervous system and demonstrate that the Drosophila larval olfactory system is a powerful model in which to understand distal-less functions during neurogenesis.

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Expression of the Drosophila homeobox gene, Distal‐less, supports an ancestral role in neural development

Plavicki

Squirrell

Eliceiri

et al. 2015

Developmental Dynamics

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Background Distal-less (Dll) encodes a homeodomain transcription factor expressed in developing appendages of organisms throughout metazoan phylogeny. Based on earlier observations in the limbless nematode Caenorhabditis elegans and the primitive chordate amphioxus, it was proposed that Dll had an ancestral function in nervous system development. Consistent with this hypothesis, Dll is necessary for the development of both peripheral and central components of the Drosophila olfactory system. Furthermore, vertebrate homologs of Dll, the Dlx genes, play critical roles in mammalian brain development. Results Using fluorescent immunohistochemistry of fixed samples and multiphoton microscopy of living Drosophila embryos we show that Dll is expressed in the embryonic, larval and adult CNS and PNS in embryonic and larval neurons, brain and ventral nerve cord (VNC) glia, as well as in PNS structures associated with chemosensation. In adult flies, Dll expression is expressed in the optic lobes, central brain regions and the antennal lobes. Conclusions Characterization of Dll expression in the developing nervous system supports a role of Dll in neural development and function and establishes an important basis for determining the specific functional roles of Dll in Drosophila development and for comparative studies of Drosophila Dll functions with those of its vertebrate counterparts.

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Role of proneural genes in the formation of the larval olfactory organ of Drosophila

Cited by 16 publications

References 50 publications

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

Homeobox gene distal-less is required for neuronal differentiation and neurite outgrowth in the Drosophila olfactory system

Expression of the Drosophila homeobox gene, Distal‐less, supports an ancestral role in neural development

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