Role of propolis on biochemical and hematological parameters of Oncorhynchus mykiss exposed to cypermethrin

Abstract

The protective effects of propolis on biochemical and hematological parameters were appraised in the blood of Oncorhynchus mykiss exposed to cypermethrin (CYP). The fish were subjected to three sublethal concentrations of CYP (0.0041, 0.0082 and 0.0123 ppm) and propolis (10 ppm) for 96 h. The samples were analysed to changes in the biochemical parameters, such as the metabolits: glucose, total protein, creatinine, urea, triglycerides, total cholesterol levels and the enzymes: Alanine Aminotransferase (ALT), Alanine Aminotransferase (AST), Alkaline Phosphatase (ALP), Lactate Dehydrogenase (LDH), amilase, Gamma Glutamyltransferase (GGT) and the electrolites: chloride, sodium, potassium, calcium, phosphate and ferric. Also, hematological parameters including total leucocyte count, granulocytes, agranulocytes and the erytrocyte count: Hemoglobin, hematocrit and the erytrocyte indexes: Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were tested in fish blood. The data of this investigation showed that CYP had a negative effect on the biochemical and hematological variables of the fish; these toxic effects were neutralised by the application of propolis. The results suggest that propolis can be effective in the prevention of CYP-induced toxicity in rainbow trout, especially hematopoiesis.

Hematological Parameters of Freshwater Fish Anabas Testudineus after Sublethal Exposure to Cypermethrin

“…The results obtained in this study showed decreases in RBC counts, Hb and HCT levels of fish exposed to increased concentrations of cypermethrin. Similar reduction in RBC counts and Hb levels had been also reported in Oncorhynchus mykiss [16,17), Labeo rohita [18,19], Sebastes schlegeli [20], Prochilodus lineatus [21] exposed to cypermethrin. The significant reduction in RBC counts may be due to disruptive action of the cypermethrin on the erythropoietic tissue [22].…”

“…On 14 th and 21 st days of exposure, WBC count decreased. The increases in WBC counts (leucocytosis) were reported in Clarias gariepinus [8], L. rohita [18,19], Cyprinus carpio [9], O. mykiss [17] exposed to cypermethrin. But, the decreases in WBC counts (leucopenia) were also described in the O. mykiss [16,24] exposed to cypermethrin.…”

Sublethal behavioral and biochemical toxicity of cypermethrin in juvenile Oreochromis niloticus in a static bioassay

“…This might have been caused by glycogen storage deficiency and hepatic dysfunction induced by prolonged sublethal exposure. Similar decreases in triglycerides and cholesterol were recorded from CYP sublethal stress on Oncorhynchus mykiss (Walbaum) (Orun et al 2014). Some other researchers also report increased values of triglyceride and cholesterol from sublethal CYP exposure (Ojutiku et al 2013, Bhanu andDeepak 2015).…”

Biochemical and histopathological effect of propolis and nanopropolis supplementation on alleviating dietary Microcystis aeruginosa toxicity on Nile tilapia, Oreochromis niloticus

“…The results of this study are in harmony with the results of Selamoglu et al (2015) who indicated that ALT, AST, values increased when exposed Cyprinus carpio to arsenic but reduced by combination of arsenic and propolis. As well as Orun et al (2014) showed that application of propolis neutralized cypermethrin (CYP) negative effect on the biochemical variables of the fish such as: the enzymes: ALT, AST, ALP. Concerning to creatinine and urea which are classical monitoring indices for kidney function and renal structural integrity their values increased significantly in group of fish fed on diet mixed with M. aeruginosa cells compared to other groups.…”

“…The results of this study are in harmony with Selamoglu et al (2015) who indicated that urea value increased when exposed (Cyprinus carpio, Linnaeus 1758) to arsenic but reduced by combination of arsenic and propolis. As well as Orun et al (2014) showed that application of propolis neutralized cypermethrin (CYP) negative effect on the biochemical variables of the Oncorhynchus mykiss such as: creatinine, urea levels. Indeed, fish histopathology is commonly used as a biomarker to assess water quality and prospective hazard.…”